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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 19,273 results for: Met;

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Showing: 701 - 720 of 19273 results

  1. pALS3-Ma-sfGFP-TAG150

    Plasmid
    #212121
    Purpose
    Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-TAG150 and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    sfGFP-150TAG
    M. alvus Pyl-tRNA(6)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    N150TAG
    Promoter
    araC and lpp
    Available Since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212121-plasmid-map-sequence-id-444186

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  2. pBK-Ma pylRS nitroY/haloY-F5

    Plasmid
    #212125
    Purpose
    Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control for Ma Pyl tRNA-synthetase selections.
    Depositor
    Ryan Mehl
    Insert
    M. alvus PylRS 3-NY/HaloY F5
    Expression
    Bacterial
    Mutation
    CTG125CTT, N166A V168S, A223C, W239R
    Promoter
    GlnS (constitutive)
    Available Since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    212125-plasmid-map-sequence-id-443626

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  5. pALS3-Ma-SUMO-sfGFP-WT

    Plasmid
    #212122
    Purpose
    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    SUMO-sfGFP-WT
    M. alvus Pyl-tRNA(6)
    Tags
    His6 and SUMO
    Expression
    Bacterial
    Promoter
    araC and lpp
    Available Since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212122-plasmid-map-sequence-id-444187

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  15. pALS3-Ma-SUMO-TAG35-sfGFP

    Plasmid
    #212120
    Purpose
    Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-TAG35-sfGFP and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    SUMO-TAG35-sfGFP
    M. alvus Pyl-tRNA
    Tags
    His6 and SUMO-TAG35
    Expression
    Bacterial
    Mutation
    E35TAG (within SUMO encoding sequence)
    Promoter
    araC and lpp
    Available Since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212120-plasmid-map-sequence-id-444184

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  20. pAS

    Plasmid
    #140008
    Purpose
    Plasmid with 4xMmaPylT cassette to receive genes of interest for amber suppression; for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021
    Insert
    4xPylT (Mma) cassette
    Expression
    Mammalian
    Mutation
    U25C
    Promoter
    h7SK
    Available Since
    May 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    140008-plasmid-map-sequence-id-296092

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Showing: 701 - 720 of 19273 results