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mAmetrine-Profilin-10

Plasmid

#56549

Purpose

Localization: Cytoskeleton, Excitation: 406, Emission: 526

Depositor

Michael Davidson

Insert

Profilin

Tags

mAmetrine

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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DNMT3A-dCas9

Plasmid

#100090

Purpose

DNMT3A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

Insert

DNMT3A (DNMT3A Human)

Use

CRISPR

Tags

3XFLag-NLS-DNMT3A-dCas9-NLS

Expression

Mammalian

Mutation

includes aa 602-912

Promoter

CMV

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-mAmetrine1.1-WPRE

Plasmid

#193340

Purpose

AAV-mediated expression of mAmetrine1.1 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

mAmetrine1.1

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pMT-Hrp48-ADARcd-V5

Plasmid

#81172

Purpose

identification of cell-specific targets of RNA binding proteins

Depositor

Michael Rosbash

Article

McMahon et al Cell. 2016 Mar 30. pii: S0092-8674(16)30

Insert

Hrp48-ADARcd fusion (Hrb27C Fly)

Expression

Insect

Promoter

Drosophila metallothionein promoter

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pdest METTL1-WT Neo

Plasmid

#223058

Purpose

METTL1 gene expression in mammalian cells

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

METTL1 (METTL1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pWS5394-CRISPRai-MET17

Plasmid

#182833

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - MET17 marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pSBbi Bla mAmetrine

Plasmid

#183257

Purpose

Stable expression of blue fluorescent mAmetrine protein in a Sleeping Beauty transposon vector.

Depositor

Alexander McLellan

Article

He et al Int J Mol Sci. 2020 Dec 4;21(23). pii: i

Insert

mAmetrime

Expression

Mammalian

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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YIp ADE2-MET17

Plasmid

#122906

Purpose

ADE2-MET17 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5 GST MetaP2

Plasmid

#112750

Purpose

express HA-GST-FRB-MetaP2

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

HA-GST-FRB-MetaP2 (METAP2 Human)

Tags

GST

Expression

Mammalian

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40

Plasmid

#101061

Purpose

AAV vector expressing CaMPARI2_F391W (Kd = 110nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Eric Schreiter

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Has Service

AAV1

Insert

CaMPARI2-F391W

Use

AAV

Tags

FLAG-HA-myc and NES_his

Promoter

hsyn (synapsin-1)

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pDZ514 pURA MET25p PP7-PS-2x-yeGFP

Plasmid

#72230

Purpose

Uracil selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoter

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

PP7-PS-2x-yeGFP

Expression

Yeast

Promoter

MET25

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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METTL3-P1

Plasmid

#237825

Purpose

Study the role and mechanism of the METTL3-P1 3' UTR in regulation gene function.

Depositor

Angela Ting

Article

Le et al Cell Rep. 2026 Jan 13;45(1):116839. doi:

Insert

METTL3-P1 3'UTR (METTL3 Human)

Expression

Mammalian

Available Since

Jan. 20, 2026

Availability

Academic Institutions and Nonprofits only

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METTL3-P2

Plasmid

#237826

Purpose

Study the role and mechanism of the METTL3-P2 3' UTR in regulation gene function.

Depositor

Angela Ting

Article

Le et al Cell Rep. 2026 Jan 13;45(1):116839. doi:

Insert

METTL3-P2 3'UTR (METTL3 Human)

Expression

Mammalian

Available Since

Jan. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pMetEGFP

Plasmid

#216330

Purpose

PMetE promoter driving expression of sfGFP

Depositor

Mark Styczynski

Article

Piorino et al ACS Synth Biol. 2023 Aug 18;12(8):2487-2

Insert

Superfolder green fluorescent protein

Expression

Bacterial

Available Since

May 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Shim_MetRS_440.109_TP_GFP_pK7fWG2_IVD_FP611

Plasmid

#228378

Purpose

Expresses N-term transit peptide from Sapria himalayana Methionine aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 440.10; determination of S. himalayana MetRS target localization

Depositor

Daniel Sloan

Article

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

N-terminal transit peptide from S. himalayana MetRS evm.model.fragScaff scaffold 440.10

Tags

Green Florescent Protien

Expression

Plant

Available Since

Feb. 26, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pMetR

Plasmid

#216329

Purpose

T7 promoter driving expression of metR

Depositor

Mark Styczynski

Article

Piorino et al ACS Synth Biol. 2023 Aug 18;12(8):2487-2

Insert

MetR

Expression

Bacterial

Available Since

Dec. 17, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pM-mAmetrine

Plasmid

#228617

Purpose

Mammalian expression of mAmetrine and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mAmetrine

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD02_CBASS_Methylosarcina_fibrata

Plasmid

#224406

Purpose

For expression of the type I CBASS system encoded by Methylosarcina fibrata

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

4TM_MfCdnH_AGS-C

Expression

Bacterial

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_ASPSCR1_METRNL

Plasmid

#205775

Purpose

Express mEGFP-tagged fusion protein, ASPSCR1_METRNL from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

ASPSCR1_METRNL (ASPSCR1, METRNL Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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METTL3

Plasmid

#155725

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

METTL3 (METTL3 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

H330Q compared to BC052244, no mutations compared…

Promoter

EF-1a

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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