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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,029 results for: T7

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Showing: 701 - 720 of 11029 results

  2. pSm-Nuclease

    Plasmid
    #132431
    Purpose
    Expresses Serratia marcescens nuclease in E. coli using T7 promoter
    Depositor
    Mark Arbing
    Insert
    Serratia marcescens nuclease (codon optimized)
    Tags
    None
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    132431-plasmid-map-sequence-id-258770

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  3. T7RNAP Cyslight A188C

    Plasmid
    #205083
    Purpose
    T7RNAP protein expression
    Depositor
    Smita Patel
    Insert
    T7RNAP Cyslight A188C (T7p07 Bacteriophage T7)
    Expression
    Bacterial
    Mutation
    C125S, C347S, C510S, C515S, C530S, C723S N88C
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    205083-plasmid-map-sequence-id-413708

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  4. T7RNAP Cyslight V597C

    Plasmid
    #205080
    Purpose
    T7RNAP protein expression
    Depositor
    Smita Patel
    Insert
    T7RNAP Cyslight V597C (T7p07 Bacteriophage T7)
    Expression
    Bacterial
    Mutation
    C125S, C347S, C510S, C515S, C530S, C723S V597C
    Available Since
    Aug. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    205080-plasmid-map-sequence-id-405327

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  5. T7RNAP Cyslight V166C

    Plasmid
    #205079
    Purpose
    T7RNAP protein expression
    Depositor
    Smita Patel
    Insert
    T7RNAP Cyslight V166C (T7p07 Bacteriophage T7)
    Expression
    Bacterial
    Mutation
    C125S, C347S, C510S, C515S, C530S, C723S V166C
    Available Since
    Aug. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    205079-plasmid-map-sequence-id-405325

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  6. C95m

    Plasmid
    #121001
    Purpose
    MoClo golden gate assembly CD part for T7 RNA polymerase (use with P18m). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    T7 RNA polymerase (Andy please check!)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121001-plasmid-map-sequence-id-236130

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  15. pKP2085

    Plasmid
    #167606
    Purpose
    Express ColicinB-V5-TEV-T7-mature MBP
    Depositor
    Kathleen Postle
    Insert
    cba-malE translational fusion
    Expression
    Bacterial
    Mutation
    cba ORF fused to V5 tag, TEV protease site, T7 ta…
    Available Since
    July 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167606-plasmid-map-sequence-id-335991

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  16. pET29b-H6_Streptavidin_sfGFP

    Plasmid
    #124296
    Purpose
    His6-tagged Streptavidin GFP fusion protein
    Depositor
    Mark Arbing
    Insert
    His6 tag - TEV protease site - T7 tag - Streptavidin (15-159) - superfolder GFP
    Tags
    His6 and T7 tags
    Expression
    Bacterial
    Available Since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    124296-plasmid-map-sequence-id-240453

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  17. pH6-TEV-mCherry

    Plasmid
    #132430
    Purpose
    Expresses His6-tagged mCherry in E. coli using T7 promoter
    Depositor
    Mark Arbing
    Insert
    mCherry
    Tags
    N-terminal His6 tag with TEV protease site
    Expression
    Bacterial
    Mutation
    Codon optimized
    Promoter
    T7
    Available Since
    Oct. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    132430-plasmid-map-sequence-id-258769

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  18. Click editor (CE1) mRNA expression vector - T7-5'UTR-PCV2-nCas9-EcKlenow-3'UTR-polyA (CJT472)

    Plasmid
    #217808
    Purpose
    Expression vector for mRNA production of the click editor construct (CE1; PCV2-nCas9-EcKlenow)
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    5'UTR-PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(exo-)-BPNLS-3'UTR
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217808-plasmid-map-sequence-id-489173

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  19. p15TV-L

    Plasmid
    #26093
    Purpose
    SGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    Sept. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
    26093-plasmid-map-sequence-id-52817

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  20. pYZ205

    Plasmid
    #104586
    Purpose
    Express SNAP-6His-CBD
    Depositor
    New England Biolabs, Yinhua Zhang
    Insert
    SNAP-6His-CBD
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    104586-plasmid-map-sequence-id-266802

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Showing: 701 - 720 of 11029 results