We narrowed to 10,112 results for: transfer
-
Plasmid#197510PurposeCbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A/A61R) (to be used with SpRY C-term constructs)DepositorInsertAAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A/A61R)[N-term]-NpuN-BPNLS-[ITR]
UseAAV and CRISPRTagsBPNLS and NpuN(intein)-BPNLSMutationABE8e mutations in TadA and nSpRY(D10A/A61R)PromoterCbhAvailable SinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-pCbh-SpRY[C-term]-gRNAentry (CA20)
Plasmid#197511PurposeCbh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpRY and BsmBI entry cassette to clone SpCas9 gRNA spacerDepositorInsertAAV-[ITR]-pCbh-BPNLS-NpuC-SpRY[C-term]-BPNLS-gRNA[BsmBI]-pU6-[ITR]
UseAAV and CRISPRTagsBPNLS and BPNLS-NpuC(intein)MutationC-terminal mutations of SpRY(L1111R/D1135L/S1136W…PromoterCbhAvailable SinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-ChrimsonR-tdTomato
Plasmid#99231PurposeAAV-mediated expression of ChrimsonR-tdTomato under the CaMKII promoter. tdTomato has codons varied between the first and second tandem repeats to reduce recombination. Using SV40 signal.DepositorInsertChrimsonR-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationChrimson K176R mutantPromoterCaMKIIAvailable SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-HA-HCARD-P2A-mCitrine
Plasmid#228902PurposeFor AAV packaging and Cre-dependent expression of a peripherally restricted DREADDDepositorHas ServiceAAV8Inserthydroxycarboxylic acid receptor 2 (Hcar2 Mouse)
UseAAV and Synthetic BiologyTagsHA and P2A mCitrineExpressionMammalianMutationR108KPromoterChicken Beta ActinAvailable SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_DA1h
Plasmid#113050Purposeexpress the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h in neuronsDepositorHas ServiceAAV Retrograde and AAV9InsertGPCR activation based DA sensor GRAB_DA1h (DRD2 Human)
UseAAVPromoterhSynAvailable SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-FLEX-tdTomato-WPRE
Plasmid#51503PurposeCan be used to generate AAV virus that will express tdTomato in the presence of CreDepositorInserttdTomato
UseAAVPromoterpCAGAvailable SinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAVtri-CMV-eGFP
Plasmid#230932PurposeUsed for producing AAV-CMV-eGFP viruses with the AAVtri triple-plasmid system. This system combines the use of the mini-pHelper-1.0 and AAV helper plasmids carrying different AAV serotypes.DepositorInserteGFP
UseAAVPromoterCMVAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Lenti-CMV-PEmax-IRES-ZeoR
Plasmid#207353PurposeLentiviral transfer plasmid containing CMV-driven expression cassette encoding PEmax enzyme for prime editing.DepositorInsertPrime editor max (PEmax) enzyme
UseLentiviralAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-Archon1-EGFP
Plasmid#108417PurposeAAV production plasmid encoding for Archon1 fluorescent voltage reporterDepositorInsertArchon1-EGFP
UseAAVExpressionMammalianPromoterCaMKIIAvailable SinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP7f-WPRE
Plasmid#104488PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoterDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV8, and AAV9InsertjGCaMP7f
UseAAVTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianPromoterSynapsinAvailable SinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-DreO-bGHpA
Plasmid#50363PurposeCan be used to generate AAV virus that will express DreO recombinase in neurons from the synapsin promoterDepositorHas ServiceAAV Retrograde and AAV5InsertDreO recombinase
UseAAVTagsnoneMutationSequence optimized for expression in mammalian ce…PromoterhSyn1Available SinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a DIO-rsChRmine-oScarlet-WPRE
Plasmid#183528PurposeOptogeneticsDepositorInsertrsChRmine-oScarlet
UseAAVMutationI146M/G174SPromoterEf1aAvailable SinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-∆Hapln1
Plasmid#200778PurposeExpresses mouse HAPLN1 mutant lacking the lectican binding domain and fused with cysteine-free GFP (cfGFP) under synapsin promoterDepositorHas ServiceAAV8InsertHyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)
UseAAVTagscfGFPMutationdeleted lectican binding domain (aminoacids 40-15…PromoterSynapsinAvailable SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSCAR_Cas9-hygro_GFP
Plasmid#162075Purpose3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and hygro resistanceDepositorInsertsCas9-2a-hygromycin B phosphotransferase
EGFP
UseCRISPR, Cre/Lox, and LentiviralExpressionMammalianMutationcodon-optimized for expression in M. musculusPromoterEF1a and sv40Available SinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-tdTomato-WPRE
Plasmid#51506PurposeCan be used to generate AAV virus that will express tdTomato in neurons from the synapsin promoterDepositorHas ServiceAAV5InserttdTomato
UseAAVPromoterphSyn1Available SinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV:ITR-EF1aCore-SaCas9-single(U6-sgRNA(backbone))-ITR
Plasmid#207880PurposeAAV vector encoding EF1a core promoter-driven SaCas9 and single sgRNA cloning site w/ the engineered Sa Guide scaffold variant (BbsI for sgRNA cloning).DepositorTypeEmpty backboneUseAAV and CRISPRExpressionMammalianAvailable SinceNov. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Tnnt2-GFP-LA
Plasmid#206198PurposeExpresses GFP-tagged Lamin-A specifically in cardiomyocytes by the Tnnt2 promoterDepositorAvailable SinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-rsChRmine-oScarlet-WPRE
Plasmid#183522PurposeOptogeneticsDepositorInsertrsChRmine-oScarlet
UseAAVMutationI146M/G174SPromoterCaMKIIaAvailable SinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-FlpO-bGHpA
Plasmid#51669PurposeCan be used to generate AAV virus that will express the FlpO recombinase gene in neurons from the synapsin promoter.DepositorHas ServiceAAV RetrogradeInsertFlpO recombinase gene
UseAAVExpressionMammalianPromoterhSyn1Available SinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEJS1096 Dual-sgRNA.Design 1
Plasmid#159538PurposeDelivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vectorDepositorInsertNme2Cas9 nuclease with two guide RNA cassettes with promoters
UseAAV, CRISPR, and Mouse TargetingTagsNLSExpressionMammalianPromoterU1aAvailable SinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only