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pcDNA3.1-VinculinI997A_TurboID_Venus

Plasmid

#200274

Purpose

Expressing actin-binding-deficient vinculin mutant with co-localization labeler TurboID and fluorescence reporter Venus

Depositor

Brenton Hoffman

Article

Tao et al Dev Cell. 2023 Mar 10:S1534-5807(23)0007

Insert

VinculinI997A_TurboID_Venus

Expression

Mammalian

Mutation

change isoleucine to alanine at 997 of vinculin

Promoter

T7

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6p-hDcp2

Plasmid

#72216

Purpose

bacterial expression of GST-tagged human Dcp2

Depositor

Megerditch Kiledjian

Article

Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

Insert

Dcp2 (DCP2 Human)

Tags

GST

Expression

Bacterial

Mutation

F146Y and E198K (please see depositor's comm…

Promoter

tac

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
pJSX-GL3

Plasmid

#11980

Purpose

Reporter gene with mouse c-jun promoter and point mutation of MEF2 binding site

Depositor

Ron Prywes

Article

Clarke et al Mol Cell Biol. 1998 Feb . 18(2):1065-73.

Insert

c-jun promoter (Jun Mouse)

Use

Luciferase

Tags

Luc

Expression

Mammalian

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
Twitch-2C pcDNA3

Plasmid

#104501

Purpose

fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-2C

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pJSTX-GL3

Plasmid

#11982

Purpose

Reporter gene with mouse c-jun promoter and point mutation of MEF2 and AP1 binding sites

Depositor

Ron Prywes

Article

Clarke et al Mol Cell Biol. 1998 Feb . 18(2):1065-73.

Insert

c-jun promoter (Jun Mouse)

Use

Luciferase

Tags

Luc

Expression

Mammalian

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pVC-Ds-ubb:MCP-p65-HSF1-2a-Cerulean-Ds

Plasmid

#119074

Purpose

Zebrafish ubiquitin promoter driving MCP-p65-HSF1 with Cerulean reporter

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

ubb:MCP-p65-HSF1-2a-Cerulean

Expression

Bacterial

Promoter

Zebrafish ubiquitin promoter

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-ubb:Com-KRAB-2a-Cerulean-Ds

Plasmid

#119075

Purpose

Zebrafish ubiquitin promoter driving Com-KRAB with Cerulean reporter

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

ubb:Com-KRAB-2a-Cerulean

Expression

Bacterial

Promoter

Zebrafish ubiquitin promoter

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV/MASC_(GLuc)_INT

Plasmid

#68440

Purpose

Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/ MASC expression backbone.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

INT construct_bearing three PP7 Stem-loops

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-JNKAR1-NES

Plasmid

#84632

Purpose

Unimolecular, FRET-based JNK activity reporter; cytosol targeted

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

ECFP-FHA1-JNKsubDD-Citrine-NES

Tags

6xHis, Citrine, ECFP, Nuclear export signal (NES)…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pILGFP97

Plasmid

#83551

Purpose

Used to evaluate the expression output of CYC1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

CYC1 promoter-yEGFP

Expression

Yeast

Promoter

CYC1

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3-24p3/LCN2-282-LUC

Plasmid

#46865

Purpose

murine 24p3 minimal promoter as a luciferase reporter

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

24p3/lipocalin 2 proximal promoter

Use

Luciferase

Promoter

24p3 minimal promoter

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only
373_MLH1 in pmiRGlo

Plasmid

#78133

Purpose

luciferase reporter for miRNA activity

Depositor

Heidi Schwarzenbach

Article

Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

Insert

MLH1 (MLH1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

3'UTR containing miRNA binding sites

Promoter

PGK

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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pJFRC210-10XUAS-FRT>STOP>FRT-myr::smGFP-OLLAS

Plasmid

#63170

Purpose

GAL4-responsive UAS “flp-On” spaghetti monster GFP-OLLAS reporter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"spaghetti monster GFP-OLLAS"

Tags

N-myristoylation

Expression

Insect

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pKD280

Plasmid

#125071

Purpose

Expression of SO_4387 under Ptac

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

SO_4387 (SO_4387 )
lacI (lacI )

Use

Synthetic Biology

Expression

Bacterial

Mutation

S286L- Please see depositor comments below

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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MG15-gal (mut p53 binding sites)

Plasmid

#16447

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

p53-binding site (mutant) (TP53 )

Use

Beta-gal reporter

Tags

beta-gal

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pEY22

Plasmid

#191028

Purpose

lim-4 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

lim-4 (lim-4 Nematode)

Expression

Worm

Mutation

None

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pEY16

Plasmid

#191022

Purpose

flp-6 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

flp-6 (flp-6 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEY18

Plasmid

#191024

Purpose

flp-19 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

flp-19 (flp-19 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEY19

Plasmid

#191025

Purpose

flp-26 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

flp-26 (flp-26 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEY21

Plasmid

#191027

Purpose

ggr-3 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

ggr-3

Expression

Worm

Mutation

None

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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