We narrowed to 71,960 results for: CaS;

Showing: 7221 - 7240 of 71960 results

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Fuw-dCas9-Dnmt3a-P2A-tagBFP

Plasmid

#84569

Purpose

Lentiviral construct to express dCas9 fused with Dnmt3a-P2A-tagBFP

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Dnmt3a (DNMT3A Human, Synthetic)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Dnmt3a-P2A-tagBFP

Plasmid

#84569

Purpose

Lentiviral construct to express dCas9 fused with Dnmt3a-P2A-tagBFP

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Dnmt3a (DNMT3A Human, Synthetic)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-dCas9-T2A-GFP

Plasmid

#53191

Purpose

Co-expresses human optimized S. pyogenes dCas9 and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized dead Cas9 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D10A and H840A

Promoter

hUbC

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-dCas9-T2A-GFP

Plasmid

#53191

Purpose

Co-expresses human optimized S. pyogenes dCas9 and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized dead Cas9 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D10A and H840A

Promoter

hUbC

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgSlc6a3

Plasmid

#159902

Purpose

Mutagenesis of Slc6a3

Depositor

Larry Zweifel

Insert

Slc6a3 (Slc6a3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgSlc6a3

Plasmid

#159902

Purpose

Mutagenesis of Slc6a3

Depositor

Larry Zweifel

Insert

Slc6a3 (Slc6a3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pC0043-PspCas13b-gRNA non target

Plasmid

#223698

Purpose

gRNA control for dPspCas13b-FTO

Depositor

Chuan He

Article

Wei et al Science. 2022 May 27;376(6596):968-973.

Insert

gRNA target sequence for luciferase control

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pC0043-PspCas13b-gRNA non target

Plasmid

#223698

Purpose

gRNA control for dPspCas13b-FTO

Depositor

Chuan He

Article

Wei et al Science. 2022 May 27;376(6596):968-973.

Insert

gRNA target sequence for luciferase control

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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Tol2_B-act_dCas9-Vp64-T2A-Citrine

Plasmid

#200248

Purpose

Zebrafish transgenics; Plasmid encodes dCas9-Vp64 and could be stably integrated in the zebrafish genome using Tol2 transgenesis

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

dCas9-Vp64-T2A-Citrine

Use

Zebrafish expression

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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Tol2_B-act_dCas9-Vp64-T2A-Citrine

Plasmid

#200248

Purpose

Zebrafish transgenics; Plasmid encodes dCas9-Vp64 and could be stably integrated in the zebrafish genome using Tol2 transgenesis

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

dCas9-Vp64-T2A-Citrine

Use

Zebrafish expression

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pKEW336-MBP-TEV-MbCas12a-33362

Plasmid

#115670

Purpose

Expresses MbCas12 (from M. bovoculi 33362) in bacteria via T7 RNAP, for purification

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

MbCas12a

Use

CRISPR

Tags

MBP-TEV

Expression

Bacterial

Promoter

T7 RNAP

Available Since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pKEW336-MBP-TEV-MbCas12a-33362

Plasmid

#115670

Purpose

Expresses MbCas12 (from M. bovoculi 33362) in bacteria via T7 RNAP, for purification

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

MbCas12a

Use

CRISPR

Tags

MBP-TEV

Expression

Bacterial

Promoter

T7 RNAP

Available Since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pKEW321-MbCas12a-RFP-GFP-array

Plasmid

#115661

Purpose

Encodes an CRISPR array for MbCas12a targeting superfolder GFP and mRFP1

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

GFP and RFP array for MbCas12

Use

CRISPR

Expression

Bacterial

Promoter

Plac

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pKEW321-MbCas12a-RFP-GFP-array

Plasmid

#115661

Purpose

Encodes an CRISPR array for MbCas12a targeting superfolder GFP and mRFP1

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

GFP and RFP array for MbCas12

Use

CRISPR

Expression

Bacterial

Promoter

Plac

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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AAVS1_dCas9-XTEN-KRAB-p2A-mCherry

Plasmid

#222107

Purpose

Donor vector for CRISPRi integration at AAVS1 with an mCherry fluorophore

Depositor

Jonathan Weissman

Article

She et al Cell. 2023 Jul 6;186(14):2977-2994.e23.

Insert

CRISPRi-kox1(KRAB) (cas9 S. pyogenes, Human)

Use

AAV and CRISPR

Tags

HA and P2A-mCherry

Expression

Mammalian

Mutation

D10A, H840A

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1_dCas9-XTEN-KRAB-p2A-mCherry

Plasmid

#222107

Purpose

Donor vector for CRISPRi integration at AAVS1 with an mCherry fluorophore

Depositor

Jonathan Weissman

Article

She et al Cell. 2023 Jul 6;186(14):2977-2994.e23.

Insert

CRISPRi-kox1(KRAB) (cas9 S. pyogenes, Human)

Use

AAV and CRISPR

Tags

HA and P2A-mCherry

Expression

Mammalian

Mutation

D10A, H840A

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pT3TS-spCas9 ABE-Umax-ex1

Plasmid

#222141

Purpose

Adenine base editor with modified TadA variant for extended window for A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Promoter

T3

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pT3TS-spCas9 ABE-Umax-ex1

Plasmid

#222141

Purpose

Adenine base editor with modified TadA variant for extended window for A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Promoter

T3

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pU6-CasRx-non-targeting (pEZ18)

Plasmid

#229049

Purpose

Cloning backbone for CasRx crRNA (U6 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-CasRx-non-targeting (pEZ18)

Plasmid

#229049

Purpose

Cloning backbone for CasRx crRNA (U6 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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