We narrowed to 19,702 results for: CAL

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pSG5-FLAG-CaMKKbeta rat K193A

Plasmid

#32450

Depositor

Anthony Means

Article

Anderson et al Cell Metab. 2008 May;7(5):377-88.

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Tags

FLAG

Expression

Mammalian

Mutation

K193A (S3V, G96R, and P108L also present)

Promoter

SV40

Available Since

April 17, 2012

Availability

Academic Institutions and Nonprofits only
FancF_N_FS_v2_Donor

Plasmid

#79891

Purpose

N-terminal tagging of human FANCF with 3xFLAG_Twin_Strep

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Type

Empty backbone

Use

CRISPR

Tags

3xFLAG_Twin_Strep

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pPRxRF3

Plasmid

#138386

Purpose

TagRFP-T knock-in cassette

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

TagRFP-T

Expression

Insect

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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NmeCas9_3XNLS_pMCSG7

Plasmid

#120078

Purpose

Bacterial expression of wtNmeCas9 with 3X NLS

Depositor

Erik Sontheimer

Article

Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

Insert

NmeCas9

Expression

Bacterial

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pEXP5-NT-CuSeCat

Plasmid

#42568

Depositor

Ivan Korendovych

Article

Mack et al J Biol Inorg Chem. 2013 Apr;18(4):411-8.

Insert

mutated calmodulin gene

Tags

Polyhistidine (6×His) region, TEV recognition sit…

Expression

Bacterial

Mutation

changed Phenylalanine 92 to Glutamine acid, Valin…

Promoter

T7

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only

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p11-3’_8xN_PAM-site2 (RTW555)

Pooled Library

#160133

Purpose

Plasmid library harboring a randomized 8nt PAM 3’ of target site 2. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 3’ PAMs (e.g. Cas9)

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Species

Synthetic

Use

CRISPR

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only
pInt_attP2_kanR

Plasmid

#205297

Purpose

Integrating plasmid for ORBIT in E. coli. Orthogonal attP site (#2).

Depositor

Scott Saunders

Article

Saunders et al bioRxiv 2023

Type

Empty backbone

Use

Synthetic Biology

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pInt_attP3_chlorR

Plasmid

#205298

Purpose

Integrating plasmid for ORBIT in E. coli. Orthogonal attP site (#3).

Depositor

Scott Saunders

Article

Saunders et al bioRxiv 2023

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pInt_attP1_chlorR

Plasmid

#205295

Purpose

Integrating plasmid for ORBIT in E. coli. Standard attP site (#1).

Depositor

Scott Saunders

Article

Saunders et al bioRxiv 2023

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pFloxin-IRES

Plasmid

#24554

Depositor

Jeremy Reiter

Article

Singla et al Nat Methods. 2010 Jan . 7(1):50-2.

Type

Empty backbone

Use

Cre/Lox and Mouse Targeting

Available Since

May 27, 2010

Availability

Academic Institutions and Nonprofits only

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pRN3P-NLS-EGFP-Btgh-3NLS

Plasmid

#194469

Purpose

Encodes for bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.

Depositor

Mathieu Boulard

Article

Formichetti et al Dev Cell 10 Jan 2024

Insert

BtGH84

Use

For in vitro transcription from t3 promoter after…

Tags

SV40 Nuclear Localization Signal, SV40 Nuclear Lo…

Expression

Bacterial

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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Epac-S-H81

Plasmid

#170339

Purpose

GFPDel(UST)_EPACdDEPCD_mRFP biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

Depositor

Kees Jalink

Article

van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.

Insert

GFPDel(UST)_EPACdDEPCD_mRFP (RAPGEF3 Human)

Expression

Mammalian

Mutation

Deletion of DEP domain, Catalytically dead T781A …

Promoter

CMV

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-AICD-NLS-IRES-hrGFP

Plasmid

#107543

Purpose

AAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Localisation Signal (NLS: CCAAAAAAGAAGAGAAAGGTA) at C-term end and IRES-mediated co-expression of hrGFP

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Insert

AICD-NLS (APP Human)

Use

AAV

Mutation

None

Promoter

CMV

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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MTK234_033

Plasmid

#123996

Purpose

Encodes the spCas9 sgRNA1 hAAVS1 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA2 -hAAVS1

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK234_034

Plasmid

#123997

Purpose

Encodes the spCas9 sgRNA1 hAAVS1 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA3 -hAAVS1

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pRD134

Plasmid

#132378

Purpose

contains the sequence of mScarlet for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

mScarlet

Use

Greengate entry plasmid

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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p4g3R

Plasmid

#107958

Purpose

RFP reporter [PT7(-3G)-RBSB0034, low copy number]

Depositor

Jim Haseloff

Article

Boehm et al Nat Commun. 2018 Feb 22;9(1):776. doi: 1

Insert

mRFP1

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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Mouse Liver CRISPR Knockout Library

Pooled Library

#192824

Purpose

Knockout library targeting 13,189 of the 13,266 protein-coding genes expressed in the mouse liver.

Depositor

Kristin Knouse

Article

Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only
pLViN-iRFP670-α-cateninA+

Plasmid

#229703

Purpose

Lentiviral expression of iRFP670-alpha-cateninA+ in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CTNNA1 (alpha-catenin) Human (CTNNA1 Human)

Use

Lentiviral

Tags

iRFP670

Expression

Mammalian

Mutation

amino acids 670-673, RAIM--> GSGS

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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PKAsub(TA)-TagRFPT-CAAX

Plasmid

#87740

Purpose

Negative-control PKA substrate fragment for bimFLINC-AKAR, targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).

Depositor

Jin Zhang

Article

Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

Insert

PKAsub(TA)-TagRFPT-CAAX

Tags

6xHis, C-terminal targeting sequence from Kras, T…

Expression

Mammalian

Mutation

Target Thr residue in substrate domain mutated to…

Promoter

CMV

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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