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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 17,881 results for: puromycin

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Showing: 7321 - 7340 of 17881 results

  1. pGBW-m4133908

    Plasmid
    #152258
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 nsp8
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;HA
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    June 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152258-plasmid-map-sequence-id-300228

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  2. pGBW-m4252975

    Plasmid
    #153873
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153873-plasmid-map-sequence-id-300418

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  3. pGBW-m4252831

    Plasmid
    #153792
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 ORF6 protein
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 ORF6 protein (ORF6 Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;3xFlag
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    153792-plasmid-map-sequence-id-301683

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  5. p667-UBC-AAK1-V5_IDG-K

    Plasmid
    #135257
    Purpose
    Gateway destination clone of AAK1 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    AAK1-V5 (AAK1 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135257-plasmid-map-sequence-id-267904

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  6. p667-UBC-AAK1-V5-miniTurbo_IDG-K

    Plasmid
    #135255
    Purpose
    Gateway destination clone of AAK1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    AAK1-V5-miniTurbo (AAK1 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135255-plasmid-map-sequence-id-267913

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  7. pEBTetD-SNAP-Centrin2

    Plasmid
    #136785
    Purpose
    Mammalian expression of the centrosomal protein Centrin2 N-terminally fused to SNAP-tag
    Depositor
    Kai Johnsson
    Publication
    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SNAP-Centrin2 (CETN2 Human, Synthetic)
    Tags
    FLAG-tag / His-tag
    Expression
    Mammalian
    Promoter
    CMV-TetO2
    Available since
    March 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    136785-plasmid-map-sequence-id-276017

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  8. pT2Apuro-TRE-synPCB2.0

    Plasmid
    #139481
    Purpose
    Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.
    Depositor
    Kazuhiro Aoki
    Publication
    Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7
    Use
    Tol2 transposon donor vector
    Expression
    Mammalian
    Promoter
    Tet responsive element
    Available since
    March 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    139481-plasmid-map-sequence-id-276016

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  11. pEBTet-SNAP-Cep290

    Plasmid
    #136804
    Purpose
    Mammalian expression of the centrosomal protein Cep290 N-terminally fused to SNAP-tag
    Depositor
    Kai Johnsson
    Publication
    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SNAP-Cep290 (CEP290 Human, Synthetic)
    Tags
    FLAG-tag / His-tag
    Expression
    Mammalian
    Promoter
    CMV-TetO2
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    136804-plasmid-map-sequence-id-275879

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  12. pEBTet-SNAP-γ-tubulin

    Plasmid
    #136799
    Purpose
    Mammalian expression of the centrosomal protein _-tubulin N-terminally fused to SNAP-tag
    Depositor
    Kai Johnsson
    Publication
    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SNAP-TUBG (TUBG1 Human, Synthetic)
    Tags
    FLAG-tag / His-tag
    Expression
    Mammalian
    Promoter
    CMV-TetO2
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    136799-plasmid-map-sequence-id-275873

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  13. pEBTet-SNAP-Cep41

    Plasmid
    #136794
    Purpose
    Mammalian expression of the centrosomal protein Cep41 N-terminally fused to SNAP-tag
    Depositor
    Kai Johnsson
    Publication
    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SNAP-Cep41 (CEP41 Human, Synthetic)
    Tags
    FLAG-tag / His-tag
    Expression
    Mammalian
    Promoter
    CMV-TetO2
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    136794-plasmid-map-sequence-id-275860

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  19. pCC_03 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9-NLS-2A-Puro-WPRE

    Plasmid
    #139088
    Purpose
    Expresses human codon-optimized xCas9 3.7 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    xCas9 3.7
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    A262T, R324L,S409I, E480K, E543D, M694I, E1219V
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139088-plasmid-map-sequence-id-273884

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Showing: 7321 - 7340 of 17881 results