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pDONR223-AKT3

Plasmid

#23539

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

AKT3 (AKT3 Human)

Use

Gateway Cloning

Mutation

S2R

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRE

Plasmid

#139089

Purpose

Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

Depositor

Neville Sanjana

Article

Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

Insert

xCas9-NG

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

A262T,R324L, S409I, E480K, E543D, M694I, L1111R, …

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pSH-Csy4-T2A-SaRFN

Plasmid

#85755

Purpose

Expresses S.aureus FokI-dCas9-NLS with a 36 amino acid GSAT linker fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Backbone derived from pSQT1601 (Addgene #53369).

Depositor

Lawrence Stanton

Article

Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

Insert

Csy4-T2A-FokI-SadCas9

Use

CRISPR

Tags

3xHA tag, Csy4, and FokI fusion via 36 amino acid…

Expression

Mammalian

Mutation

D10A, N580A

Promoter

CAG

Available Since

Jan. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-F30-TAR Variant-1

Plasmid

#129406

Purpose

circular TAR Variant-1

Depositor

Samie Jaffrey

Article

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

circular F30-TAR Variant-1

Expression

Mammalian

Promoter

U6

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR221-NFAT5

Plasmid

#79506

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NFAT5 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

NFAT5 (NFAT5 Human)

Use

Gateway Cloning

Promoter

no Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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C32A

Plasmid

#27802

Depositor

Steven Vogel

Article

Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

Insert

Cerulean-32-Amber

Use

Fret donor control

Tags

Amber and Cerulean

Expression

Mammalian

Available Since

May 23, 2011

Availability

Academic Institutions and Nonprofits only
pDONR223-INSRR

Plasmid

#23924

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

INSRR (INSRR Human)

Use

Gateway Cloning

Mutation

P14H

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-SCYL2

Plasmid

#23458

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

SCYL2 (SCYL2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PINK1

Plasmid

#23504

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PINK1 (PINK1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pYL112-19

Plasmid

#15459

Depositor

Chia Lee

Article

Luong et al J Microbiol Methods. 2007 Jul . 70(1):18

Insert

S. aureus phage L54a integrase

Use

S.aureus-e. coli shuttle vector

Available Since

Oct. 9, 2007

Availability

Academic Institutions and Nonprofits only
pMMLV-EBFP-P2A-Puro

Plasmid

#160227

Purpose

Expresses EBFP in mammalian cells

Depositor

Jason Sheltzer

Article

Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

Insert

EBFP-P2A-Puro

Use

Retroviral

Expression

Bacterial and Mammalian

Promoter

LTR

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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4xHRE-MinTK-CRE

Plasmid

#141144

Purpose

lentiviral expression vector to generate a hypoxia fate-mapping system

Depositor

Daniele Gilkes

Article

Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

Insert

4xHRE-MinTK-CRE

Use

Lentiviral

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCAS9-TPC

Plasmid

#61478

Purpose

Binary expression vector with A.th. codon-optimized Cas9 for conventional cloning

Depositor

Holger Puchta

Article

Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

Insert

Cas9

Use

CRISPR

Expression

Plant

Promoter

PcUbi4-2

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pWJB30

Plasmid

#126505

Purpose

Salmonella typhimurium diguanylate cyclase AdrA cloned as AdrA-8xHis in pBAD30

Depositor

Ute Romling

Article

Simm et al Mol Microbiol. 2004 Aug;53(4):1123-34. d

Insert

adrA-8xHis

Tags

8xHis

Expression

Bacterial

Promoter

PBAD30

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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MSP2266

Plasmid

#70705

Purpose

Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Inserts

mammalian codon-optimized Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)

Use

CRISPR

Tags

NLS-3xFLAG

Expression

Bacterial

Promoter

T7

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_SST1.0

Plasmid

#208665

Purpose

Expresses the genetically-encoded fluorescent somatostatin (SST) sensor GRAB_SST1.0 in neurons

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based somatostatin (SST) sensor GRAB_SST1.0

Use

AAV

Promoter

hSyn

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2

Plasmid

#163403

Purpose

Expresses BMPR-2 in mammalican cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Expression

Mammalian

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-ULK4

Plasmid

#23849

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ULK4 (ULK4 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
FKB

Plasmid

#12499

Depositor

David Baker

Article

Dantas et al J Mol Biol. 2003 Sep 12. 332(2):449-60.

Insert

FKB

Tags

6xHis

Expression

Bacterial

Mutation

RosettaDesign was used to design sequences. Align…

Available Since

March 13, 2007

Availability

Academic Institutions and Nonprofits only
pBABE-hygro NM23 H1 WT

Plasmid

#11364

Depositor

Ronald Kahn

Article

Zhu et al Proc Natl Acad Sci U S A. 1999 Dec 21. 9

Insert

NM23 H1 (NME1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

March 1, 2006

Availability

Academic Institutions and Nonprofits only