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pCE-KdGl

Plasmid

#154880

Purpose

Episomally expresses KDM4D and GLIS1 in mammalian cells

Depositor

Hideyuki Okano

Publication

Yoshimatsu et al Stem Cell Reports. 2021 Mar 23. pii: S22

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

KDM4D, GLIS1 (KDM4D, GLIS1 Human, Callithrix jacchus)

Expression

Mammalian

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBS U6a gRNA

Plasmid

#158961

Purpose

gRNA backbone driven by zebrafish U6a promoter

Depositor

Michael Brand

Publication

Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

Inserts

zf U6a promoter
gRNA backbone

Use

CRISPR

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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Flag-FOXC1

Plasmid

#153114

Purpose

Expression of Flag tagged human FOXC1 in mammalian cells

Depositor

Stefan Koch

Publication

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXC1 (FOXC1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Lenti-eCas9

Plasmid

#140237

Purpose

Lentiviral vector for expression of high-specificity eSpCas9(1.1) and sgRNA scaffold

Depositor

Urs Greber

Publication

Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

EF-1a core

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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TetOn-CLIMP63-mCherry-eGFP

Plasmid

#139871

Purpose

Dox-inducible lentiviral expression of human CLIMP63

Depositor

Jacob Corn

Publication

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

CLIMP63 (CKAP4 Human)

Use

Lentiviral

Tags

mCherry-eGFP

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCrispr-V2-mOrange (V2mO)

Plasmid

#140206

Purpose

Lenti Crispr/Cas9 with mOrange fluorescent marker

Depositor

Jaffer Ajani

Publication

Jin et al PLoS One. 2020 Feb 13;15(2):e0228910. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

pELF1a

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCMV-ABEmax-NRCH

Plasmid

#136923

Purpose

Expresses ABEmax-NRCH in mammalian cells

Depositor

David Liu

Publication

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ABEmax-NRCH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHR-PGK-scFv GCN4-sfGFP-VP64

Plasmid

#122133

Purpose

Express a scFv which binds to GCN4, sfGFP and VP64 cassette from the PGK promoter

Depositor

Stanley Qi

Publication

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Insert

scFv GCN4-sfGFP-VP64

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

PGK

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCCLc-MND-A0201-Mart1-SABR

Plasmid

#119052

Purpose

3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains the Mart1 epitope "ELAGIGILTV" cloned via BsmBI

Depositor

David Baltimore

Publication

Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

Insert

A0201-Mart1-SABR

Use

Lentiviral

Expression

Mammalian

Promoter

MND

Available since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Tet-pLKO-puro shKRAS

Plasmid

#116871

Purpose

Tet-inducible suppression of KRAS

Depositor

William Hahn

Publication

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

shKRAS (KRAS Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1/TO

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Twin-Strep_IRES-mRuby2

Plasmid

#113885

Purpose

Mammalian (inducible) protein expression, bicistronic expression of cytosolic mRuby2

Depositor

A. Radu Aricescu

Publication

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Promoter

CMV-MIE-TetO2

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLenti-BE4GamRA-P2A-Puro

Plasmid

#112673

Purpose

Lentivirus for constitutive expression of BE4GamRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE4GamRA

Use

Lentiviral

Tags

FLAG

Mutation

NLS sequence at the N-terminus and D10A

Promoter

EF1s

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTol2-UAS-zArchon1-KGC-EGFP-ER2

Plasmid

#108427

Purpose

Codon-optimized Archon1 fluorescent voltage reporter in zebrafish expression plasmid

Depositor

Edward Boyden

Publication

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

zArchon1-KGC-EGFP-ER2

Use

Zebrafish

Promoter

UAS

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-Puro_siKO

Plasmid

#86696

Purpose

Tetracycline inducible expression of guide RNA targeted to AAVS1 locus

Depositor

Ludovic Vallier

Publication

Bertero et al Development. 2016 Dec 1;143(23):4405-441

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

H1 TO

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRG2

Plasmid

#104174

Purpose

sgRNA expression

Depositor

Jin-Soo Kim

Publication

Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

43 Articles

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LentiGuidPuro-hTP53_sgRNA-1

Plasmid

#88853

Purpose

CRISPR KO of Trp53

Depositor

Joan Massague

Publication

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp53

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS/ES-EnvA-VSVg-WPRE-pA

Plasmid

#87221

Purpose

to package wild type EnvA psudotyped lentivirus

Depositor

Fan Wang

Publication

Sakurai et al Neuron. 2016 Nov 23;92(4):739-753. doi:

Insert

EnvA-VSVg

Use

Mouse Targeting and Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available since

March 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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NPY-td-Orange2

Plasmid

#83497

Purpose

Expression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.

Depositor

Sebastian Barg

Publication

Gandasi et al PLoS One. 2015 Jun 19;10(6):e0127801. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NPY (NPY Human)

Tags

tandem mOrange2

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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vGAT-pH pcaggs SE

Plasmid

#78578

Purpose

image recycling of VGAT in live neurons

Depositor

Susan Voglmaier

Publication

Santos et al J Neurosci. 2013 Jun 26;33(26):10634-46.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

vGAT-pHluorin C-term (Slc32a1 Rat)

Tags

superecliptic pHluorin

Expression

Mammalian

Promoter

chicken actin

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pMpGWB104

Plasmid

#68558

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Publication

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

GUS

Expression

Plant

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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