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ALPK1 gRNA (BRDN0001146035)

Plasmid

#76081

Purpose

3rd generation lentiviral gRNA plasmid targeting human ALPK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ALPK1 (ALPK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ALPK1 gRNA (BRDN0001145294)

Plasmid

#76083

Purpose

3rd generation lentiviral gRNA plasmid targeting human ALPK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ALPK1 (ALPK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ALPK1 gRNA (BRDN0001146875)

Plasmid

#76084

Purpose

3rd generation lentiviral gRNA plasmid targeting human ALPK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ALPK1 (ALPK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ERN1 gRNA (BRDN0001162304)

Plasmid

#76407

Purpose

3rd generation lentiviral gRNA plasmid targeting human ERN1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ERN1 (ERN1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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pMIG-hNFATc1/bC - AVITEV

Plasmid

#74059

Purpose

retroviral expression plasmid for human NFATc1/bC with C-terminal BirA-biotinylation signal and TEV celavage site

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

human NFATc1, isoform beta-C (NFATC1 Human)

Use

Retroviral

Tags

AVI-TEV

Expression

Mammalian

Promoter

pMSCV-LTRs

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 NFKB1

Plasmid

#67126

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

NFKB1 (NFKB1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
mTFP1-Mito-7

Plasmid

#55500

Purpose

Localization: Mitochondria, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Mito (COX8A Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCRII-Topo Cav1 in situ probe

Plasmid

#45624

Depositor

Jeffrey Macklis

Article

Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

Insert

Cav1 in situ probe (Cav1 Mouse)

Use

In situ

Mutation

fragment contains bp# 1472-2124 of AB029929.1

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
pWzl_neo_DEST_flag_PIK3CA

Plasmid

#45308

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

PIK3CA (PIK3CA Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3/HIP1/ΔLD

Plasmid

#31253

Depositor

Theodora Ross

Article

Rao et al J Clin Invest. 2002 Aug . 110(3):351-60.

Insert

Huntingtin-interacting protein 1 (HIP1 Human)

Expression

Bacterial

Mutation

Deltion of nucleotides 767-1335

Available Since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only
Gcm2TEASY

Plasmid

#41031

Depositor

Nancy Manley

Article

Gordon et al Mech Dev. 2001 May;103(1-2):141-3.

Insert

Gcm2 in situ probe (Gcm2 Mouse)

Use

In situ probe

Mutation

contains nucleotides 584-1510 of GenBank accessio…

Promoter

T7 sense; SP6 antisense

Available Since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only
p6900 PHAGE-P CMVT N-HA-hE6AP III C840A

Plasmid

#37606

Depositor

Peter Howley

Article

Martinez-Noel et al Mol Cell Biol. 2012 May 29.

Insert

E6AP III C840A (UBE3A Human)

Tags

HA

Expression

Mammalian

Mutation

C840A

Promoter

CMV tet regulatable

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only
UAS::PTPRFa.1-DN-GFP

Plasmid

#37110

Depositor

Alvaro Sagasti

Article

Wang et al Curr Biol. 2012 Mar 6;22(5):373-82. Epub

Insert

UAS::PTPRFa.1-DN-GFP (ptprf Zebrafish)

Use

Tol2 gateway expression vector

Tags

EGFP

Mutation

deleted amino acids 1326-1894

Promoter

UAS-E1b

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FRB-LplA(W37I)

Plasmid

#34840

Depositor

Alice Ting

Article

Slavoff et al J Am Chem Soc. 2011 Dec 14;133(49):19769

Insert

FRB-LplA(W37I)

Tags

FLAG (internal fusion), HA, and His Tag

Expression

Mammalian

Mutation

LplA has Trp37 to Val mutation

Promoter

CMV

Available Since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-hNPPC-RFP

Plasmid

#22921

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

human c-type natruiretic peptide precursor promoter driving DsRedExpress (NPPC Human)

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pAT752

Plasmid

#68903

Purpose

NanoLuc expressed constiutively from PAM2, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pAT795

Plasmid

#68928

Purpose

NanoLuc expressed constiutively from PAM4 and GH023 RBS, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pAT782

Plasmid

#68919

Purpose

NanoLuc expressed constiutively from PAM2 and rpiL* RBS, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pAT779

Plasmid

#68916

Purpose

NanoLuc expressed constiutively from PAM2 and GH078 RBS, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pAT754

Plasmid

#68905

Purpose

NanoLuc expressed constiutively from PAM4, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge