We narrowed to 30,515 results for: Ide;

1. pC016 - LwCas13a guide expression backbone with U6 promoter

   Plasmid

   #91906

   Purpose

   Backbone for cloning LwCas13a guides under U6 promoter

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Has Service

   Cloning Grade DNA

   Insert

   LwCas13a Guide site
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. single polypeptide FPX biosensor for calcium ion

   Plasmid

   #60887

   Purpose

   FPX biosensor

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   single polypeptide FPX biosensor for calcium ion
   

   Use

   Tags

   HindIII site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. CROPseq-Guide-EFS-SpCas9-P2A-EGFP

   Plasmid

   #99248

   Purpose

   single-vector system with EGFP reporter for use in CROPseq knockout screens

   Depositor

   Alex Hewitt

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Tol2-LyzC-RacFRET-U6ac-ctrl guides

   Plasmid

   #168247

   Purpose

   "label active Rac; control for neutrophil-specific knockout"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   RacFRET
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   LyzC

   Available since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDF0584 huDisCas7-11 S1006-GGGS-D1221 U6-NT guide

   Plasmid

   #186993

   Purpose

   AAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of non-targeting crRNA guide

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

   Insert

   cas7-11-s1006-gggs-d122, non-targeting crRNA guide
   

   Use

   AAV

   Tags

   Expression

   Mutation

   s1006-gggs-d1221 deletion

   Promoter

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDF0582 huDisCas7-11 R1045-GGGS-R1122 U6-NT guide

   Plasmid

   #186991

   Purpose

   AAV transgene plasmid for Cas7-11-R1045-GGGS-R1122, with U6 promoter-driven expression of non-targeting crRNA guide

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

   Insert

   cas7-11-r1045-gggs-r1122, non-targeting crRNA guide
   

   Use

   AAV

   Tags

   Expression

   Mutation

   r1045-gggs-r1122 deletion

   Promoter

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Direct-seq_lentiGuide-Puro-tRNA-Loop2-8A8G

   Plasmid

   #157986

   Purpose

   Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

   Depositor

   Lijia Ma

   Article

   Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

   Insert

   S. pyogenes sgRNA cassette (tRNA-Loop2-8A8G)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. single polypeptide FPX biosensor for both caspase-3 and -8

   Plasmid

   #60886

   Purpose

   FPX biosensor

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   single polypeptide FPX biosensor for caspase-3 and 8
   

   Use

   Tags

   HindIII site and NES sequence LALKLAGLDIGS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

9. Tol2-LyzC-GFP-U6ac-ctrl-guides

   Plasmid

   #168240

   Purpose

   "neutrophil specific GFP with ubiquitous ctrl sgRNAs"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   control sgRNAs
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Tol2-LyzC-GFP-U6ac-rac2-guides

    Plasmid

    #168241

    Purpose

    "neutrophil specific GFP with ubiquitous rac2 sgRNAs"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    rac2 sgRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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