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Plasmid#1328DepositorInsertGPD promoter-hsp82 ts mutant (G170D) (HSP82 Budding Yeast)
UseTagsExpressionYeastMutationG170DPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pET28b-ZnF-UBPdel
Plasmid#90299Purposeprotein expressionDepositorInsertSAD1
UseTagsHis tagExpressionBacterialMutationZnF-UBP deletedPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pET28b-sad1-H79A
Plasmid#90303Purposeprotein expressionDepositorInsertSAD1
UseTagsHis tagExpressionBacterialMutationH79APromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pET28b-sad1-H85A
Plasmid#90304Purposeprotein expressionDepositorInsertSAD1
UseTagsHis tagExpressionBacterialMutationH85APromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pET28b-sad1-D159A
Plasmid#90305Purposeprotein expressionDepositorInsertSAD1
UseTagsHis tagExpressionBacterialMutationD159APromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pET28b-sad1-H405A
Plasmid#90306Purposeprotein expressionDepositorInsertSAD1
UseTagsHis tagExpressionBacterialMutationH405APromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pET28b-sad1-D422A
Plasmid#90307Purposeprotein expressionDepositorInsertSAD1
UseTagsHis tagExpressionBacterialMutationD422APromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pFA6a-3HA-TRP1
Plasmid#41599DepositorInsertTRP1 (TRP1 Budding Yeast)
UseYeast genomic targetingTags3xHA and yeast ADH1 terminatorExpressionMutationPromoterAvailable sinceMay 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GFP(S65T)-TRP1
Plasmid#41597DepositorInsertTRP1 (TRP1 Budding Yeast)
UseYeast genomic targetingTagsGFP (S65T) and yeast ADH1 terminatorExpressionMutationPromoterAvailable sinceJan. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-13Myc-TRP1
Plasmid#41601DepositorInsertTRP1 (TRP1 Budding Yeast)
UseYeast genomic targetingTags13Myc and yeast ADH1 terminatorExpressionMutationPromoterAvailable sinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPM28 (eroGFP CEN/ARS URA3)
Plasmid#20131DepositorInserteroGFP (KAR2 jellyfish, Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pKL06
Plasmid#104430Purposesuper Ecliptic pHluorin with C terminal mRuby2 fusion tag in yeast expression vectorDepositorInsertSuper Ecliptic pHluorin-mRuby2
UseTagsExpressionYeastMutationPromoterTef1Available sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKL07
Plasmid#104431Purposemitochondrial matrix targeted super ecliptic pHluorin with C terminal mRuby2 fusion tag in yeast expression vectorDepositorInsertmito-Super Ecliptic pHluorin-mRuby2
UseTagsExpressionYeastMutationPromoterTef1Available sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCHW00011
Plasmid#222439PurposeLevel 0, CDSns module (AATG-TTCG) containing S. cerevisiae CUP2.DepositorInsertCUP2 (Saccharomyces cerevisiae) (CUP2 Budding Yeast)
UseSynthetic BiologyTagsExpressionPlantMutationnoPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG/GFP
Plasmid#139980Purposeexpresses GFP under the promoter of CAG. Transgene flanked by AAV2 ITRDepositorInsertGreen fluorescent protein
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJCX4
Plasmid#170756PurposeExpress in mammalian cells a target protein C-terminally fused to the Mxe GryA intein - chitin binding domain (CBD) tag.DepositorTypeEmpty backboneUseTagsMxe GyrA intein - chitin binding domain (CBD)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS423-CUP1-His-Ubi
Plasmid#99541PurposeYeast episomal vector for expression of His-tagged ubiquitin; HIS3 markerDepositorInsertUBI3(1-76) (RPL40A Budding Yeast)
UseTags7xHisExpressionYeastMutationPromoterCUP1Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
YEp195-CUP1-His-Ubi
Plasmid#99540PurposeYeast episomal vector for expression of His-tagged ubiquitin; URA3 markerDepositorInsertUBI3(1-76) (RPL40A Budding Yeast)
UseTags7xHisExpressionYeastMutationPromoterCUP1Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
YEp181-CUP1-His-Ubi
Plasmid#99539PurposeYeast episomal vector for expression of His-tagged ubiquitin; LEU2 markerDepositorInsertUBI3(1-76) (RPL40A Budding Yeast)
UseTags7xHisExpressionYeastMutationPromoterCUP1Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
PHBA02
Plasmid#83536PurposeConstitutive TEF1 promoter mediated expression of the UBiC and ARO4* genesDepositorInsertpTEF1-ARO4*-CYC1t-pTEF1-UBiC-CYC1t
UseTagsExpressionYeastMutationPromoterpTEF1Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pCAG/Luci
Plasmid#139981PurposeExpresses luciferase under the promoter of CAG. Transgene flanked by AAV2 ITRDepositorInsertLuciferase
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
YEp181-CUP1-His-Smt3
Plasmid#99536PurposeYeast episomal vector for expression of His-tagged Smt3 (SUMO); LEU2 markerDepositorInsertSMT3 (SMT3 Budding Yeast)
UseTags7xHisExpressionYeastMutationPromoterCUP1Available sinceAug. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSCupSup35GFP
Plasmid#1087DepositorInsertSUP35 (SUP35 Budding Yeast)
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceAug. 9, 2005AvailabilityAcademic Institutions and Nonprofits only -
pH3FS
Plasmid#85780PurposeN-ICE plasmid pH3FSDepositorInsertloxP-HphMX-loxP-3FLAG
UseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS426-GFP-Osh2-PH
Plasmid#71373PurposeFor analysis of PH domain localization in yeastDepositorInsertOsh2-PH
UseTagsEGFPExpressionYeastMutationPleckstrin homology (PH) domain; aa 277-398PromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
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YIp128-P30-His-POL30
Plasmid#99542PurposeYeast integrative vector for expression of His-tagged POL30 (PCNA); LEU2 markerDepositorInsertPOL30 (POL30 Budding Yeast)
UseTags6xHisExpressionYeastMutationPromoterPOL30Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK3FS
Plasmid#85776PurposeN-ICE plasmid pK3FSDepositorInsertloxP-KanMX-loxP-3FLAG
UseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pH3F-GAL1
Plasmid#85775PurposeN-ICE plasmid pH3F-GAL1DepositorInsertloxP-HphMX-loxP-GAL1p-3FLAG
UseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX2TK-Osh2-PH
Plasmid#71370Purposebacterial expression of PH domain for phosphoinositide binding assayDepositorInsertOsh2-PH
UseTagsGSTExpressionBacterialMutationPleckstrin homology (PH) domain; aa 277-398PromotertacAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
UBC13-PGEX4T
Plasmid#25464DepositorUseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
YEp112-CUP1-His-Smt3
Plasmid#99537PurposeYeast episomal vector for expression of His-tagged Smt3 (SUMO); TRP1 markerDepositorInsertSMT3 (SMT3 Budding Yeast)
UseTags7xHisExpressionYeastMutationPromoterCUP1Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX2TK-Num1-PH
Plasmid#71369Purposebacterial expression of PH domain for phosphoinositide binding assayDepositorInsertNum1-PH
UseTagsGSTExpressionBacterialMutationPleckstrin homology (PH) domain; aa2563-2692PromotertacAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS426-GFP-Osh1-PH
Plasmid#71372PurposeFor analysis of PH domain localization in yeastDepositorInsertOsh1-PH
UseTagsEGFPExpressionYeastMutationPleckstrin homology (PH) domain; aa267-388PromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX2TK-Skm1-PH
Plasmid#71371Purposebacterial expression of PH domain for phosphoinositide binding assayDepositorInsertSkm1-PH
UseTagsGSTExpressionBacterialMutationPleckstrin homology (PH) domain; aa1-132PromotertacAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
YEp195-CUP1-His-Smt3
Plasmid#99538PurposeYeast episomal vector for expression of His-tagged Smt3 (SUMO); URA3 markerDepositorInsertSMT3 (SMT3 Budding Yeast)
UseTags7xHisExpressionYeastMutationPromoterCUP1Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCCL-ReDNAP (Y427A)
Plasmid#52346PurposeCEN6/ARS4 plasmid, REV1 promoter>Recoded TP-DNAP1 (Y427A)DepositorInsertRecoded TP-DNAP
UseTagsExpressionBacterial and YeastMutationPromoterREV1Available sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
ClhN-p1k-Vrg4-V5-APEX2-URA
Plasmid#207071PurposeExpressing Vrg4-V5-APEX2. Marker for late Golgi/early endosomes.DepositorInsertVrg4
UseTagsV5-APEX2ExpressionYeastMutationPromoterpVRG4Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-pADH3-4V5-APEX2-URA
Plasmid#207063PurposeBackbone plasmid containing ADH3 promoter and 4V5-APEX2 tag.DepositorInsert4xV5-APEX2
UseTagsExpressionYeastMutationPromoterpADH3Available sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-pADH3-Tgl3-4V5-APEX2-URA
Plasmid#207066PurposeExpressing Tgl3-4V5-APEX2. Marker for lipid droplets.DepositorInsertTgl3
UseTags4xV5-APEX2ExpressionYeastMutationPromoterpADH3Available sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMM0553
Plasmid#128989PurposeLEU2 integration vector of pZF(3BS)-mRUBY2DepositorInsertLeu2 5' homology-pZiF promoter-mRuby2-tADH1-Con1-LEU2-Leu2 3' homology
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
YIp128-P30-His-pol30(K164R)
Plasmid#99544PurposeYeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K164R; Leu2 markerDepositorInsertPOL30 (POL30 Budding Yeast)
UseTags6xHisExpressionYeastMutationK164RPromoterPOL30Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19-URA3-3'SEC13-GFP
Plasmid#25445DepositorInsertSec13-Green Fluorescent Protein
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTCW007
Plasmid#83525Purposepheromone mediated alpha pheromone expression in yeast from the mfalpha 1 gene and FUSJ2 promoterDepositorInsertpFUS1J2-mfalpha1
UseTagsExpressionYeastMutationPromoterpFUS1J2Available sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pN3FS-CYC1
Plasmid#85783PurposeN-ICE plasmid pH3FS-CYC1DepositorInsertloxP-NatMX-loxP-CYC1p-3FLAG
UseCre/LoxTagsExpressionYeastMutation*(see below)PromoterAvailable sinceSept. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXcX-3.6TPH-GAL4p65
Plasmid#32573DepositorInsertGal4p65
UseAdenoviralTagsExpressionMammalianMutationPromoter3.6TPHAvailable sinceJan. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pL-rpb1-E1103G
Plasmid#10989DepositorInsertrpb1-E1103G (RPO21 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationMutation 3308 A to G in the RPB1 ORF resulting in…PromoterAvailable sinceDec. 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
pMM0530
Plasmid#128987PurposePart plasmid for Zif268DBD-CRY2PHR, Type 3 (coding sequence) partDepositorInsertSV40NLS-ZiF268DBD-CRY2PHR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pH3FS-GAL1
Plasmid#85782PurposeN-ICE plasmid pH3FS-GAL1DepositorInsertloxP-HphMX-loxP-GAL1p-3FLAG
UseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only