We narrowed to 1,285 results for: HL;

1. pGG-9.47_VP1,3 #2515

   Plasmid

   #74237

   Purpose

   Modified plasmid with a different backbone that yields higher of AAV-AS vectors. Encodes VP1 and VP3 of AAV9.47

   Depositor

   Miguel Sena-Esteves

   Article

   Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2

   Inserts

   AAV2 Rep
   AAV9.47 VP1
   AAV9.47 VP3
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. Raichu-OsRac1 WT

   Plasmid

   #133262

   Purpose

   Biosensor for monitoring activation for rice small GTPase OsRac1

   Depositor

   Yoji Kawano

   Article

   Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

   Insert

   OsRac1 (LOC4325879 Oryza sativa)
   

   Use

   Tags

   CFP-lipid and Venus-CRIB

   Expression

   Plant

   Mutation

   Promoter

   Ubi

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pScarlessHD-C-3xVHH05-DsRed

   Plasmid

   #171580

   Purpose

   For scarless insertion of 3xVHH05 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

   Depositor

   Norbert Perrimon, Hidde Ploegh

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   3xVHH05 (UBE2J1 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pScarlessHD-C-3x127D01-DsRed

   Plasmid

   #171578

   Purpose

   For scarless insertion of 3x127D01 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

   Depositor

   Norbert Perrimon, Hidde Ploegh

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   3x127D01 (CXCR2 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CaMKII-GRAB_eCB2.0

   Plasmid

   #164608

   Purpose

   Express the endocannabinoid sensor GRAB_eCB2.0 in CaMKII positive neurons

   Depositor

   Yulong Li

   Article

   Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

   Insert

   Endocannabinoid sensor GRAB_eCB2.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   S383T

   Promoter

   CaMKII

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR-DMD-Donor

   Plasmid

   #60605

   Purpose

   Knock-in donor vector to insert Exon 44 in front of Exon 45 of human DYSTROPHIN (DMD)gene, include EF1a-hygromycin resistance cassette flanked by loxP sequences.

   Depositor

   Akitsu Hotta

   Article

   Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

   Insert

   Hygromycin resistant gene
   

   Use

   Knock-in donor vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pScarlessHD-N-3xVHH05-DsRed

   Plasmid

   #171581

   Purpose

   For scarless insertion of 3xVHH05 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

   Depositor

   Norbert Perrimon, Hidde Ploegh

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   3xVHH05 (UBE2J1 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAW-CD8-NbVHH05-GFP

   Plasmid

   #171576

   Purpose

   Expresses GFP-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-GFP) in fly cell cytomembrane

   Depositor

   Norbert Perrimon, Hidde Ploegh

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   CD8-NbVHH05-GFP (UBE2J1 Human)
   

   Use

   Tags

   CD8 and GFP

   Expression

   Insect

   Mutation

   Promoter

   fly actin5C promoter

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pScarlessHD-N-3x127D01-DsRed

   Plasmid

   #171579

   Purpose

   For scarless insertion of 3x127D01 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

   Depositor

   Norbert Perrimon, Hidde Ploegh

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   3x127D01 (CXCR2 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Raichu-OsRac1 CA

    Plasmid

    #133264

    Purpose

    Constitutively-active (CA) mutant of OsRac1; Acts as a positive control for small GTPases activation assays.

    Depositor

    Yoji Kawano

    Article

    Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

    Insert

    OsRac1(G19V) (LOC4325879 Oryza sativa)
    

    Use

    Tags

    CFP-lipid and Venus-CRIB

    Expression

    Plant

    Mutation

    OsRac1 G19V

    Promoter

    Ubi

    Available Since

    Jan. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Raichu-OsRac1 DN

    Plasmid

    #133263

    Purpose

    Dominant-negative (DN) mutant of OsRac1; Acts as a negative control for small GTPases activation assays

    Depositor

    Yoji Kawano

    Article

    Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

    Insert

    OsRac1(T24N) (LOC4325879 Oryza sativa)
    

    Use

    Tags

    CFP-lipid and Venus-CRIB

    Expression

    Plant

    Mutation

    OsRac1 T24N

    Promoter

    Ubi

    Available Since

    Jan. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBUN501

    Plasmid

    #50582

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Bar resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    zCas9D10A
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG and NLS

    Expression

    Mutation

    Cas9D10A nickase, was derived from the zCas9 and …

    Promoter

    AtU6-26p and Ubi1p

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCardinal-Paxillin-22

    Plasmid

    #56171

    Purpose

    Localization: Focal Adhesions, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    Paxillin
    

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pCS2.0 Tag-RFP-T-UtrCH

    Plasmid

    #101279

    Purpose

    Life imaging pCS2.0 Tag-RFP-T_UtrCH

    Depositor

    Alpha Yap

    Article

    Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

    Insert

    Utrophin (UTRN Human)
    

    Use

    Tags

    TagRFP-T

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCardinal-PMP-N-10

    Plasmid

    #56173

    Purpose

    Localization: Peroxisomes, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    PMP (PXMP2 Human)
    

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pBoBi-hALDOA

    Plasmid

    #210806

    Purpose

    For stably expression in cells

    Depositor

    Sheng-cai Lin

    Article

    Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

    Insert

    ALDOA (ALDOA Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNSSCPG

    Plasmid

    #183187

    Purpose

    PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; sfGFP-LAA expressed under T7 p

    Depositor

    Hsiao-Chun Huang

    Article

    Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1

    Inserts

    superfolder GFP
    SpmX450 and T7 181+ fusion protein
    T7 RNAP 1-179 variant and SpmX450 fusion protein
    mRFP1 and popZ fusion protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVCT-Dnd1-EGFP

    Plasmid

    #106343

    Purpose

    Lentiviral vector, mammalian expression of Dnd1-EGFP fusion protein

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    Dnd1-EGFP (Dnd1 Mouse, Synthetic)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mCardinal-NUP50-N-10

    Plasmid

    #56170

    Purpose

    Localization: Nuclear Pore, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    NUP50 (NUP50 Human)
    

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 4, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pAR-9.47_VP1,3

    Plasmid

    #74240

    Purpose

    Original plasmid described in Choudhury et al. 2015 Mol Ther. for production of AAV-AS vectors. Encodes VP1 and VP3 of AAV9.47

    Depositor

    Miguel Sena-Esteves

    Article

    Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2

    Inserts

    AAV2 Rep
    AAV9.47 VP1
    AAV9.47 VP3
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge