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Plasmid#133124PurposepL0M-PU-NoP, promotorless region used as negative controlDepositorInsertNoP promotorless region from plasmid pIJJ11268
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
sh-Myocardin
Plasmid#100768Purposeexpression of shRNA targeting MYOCDDepositorInsertsh-Myocardin
UseRNAiTagsExpressionMutationPromoterH1Available sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNB695
Plasmid#60144PurposeDisintegrator plasmid encoding MET17 promoter driven green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).DepositorInsertGrLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterMET17prAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB697
Plasmid#60146PurposeDisintegrator plasmid encoding MET17 promoter driven red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).DepositorInsertRdLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterMET17prAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSP323 pRS306 pRPS2-Mkk2DS-NLS-mCherry
Plasmid#68280PurposeSKARS: Mpk1 MAPK activity sensor: Mkk2DS (1-100) 2xNLS mCherryDepositorInsertMkk2DS NLS mCherry (MKK2 Budding Yeast)
UseTagsmCherryExpressionYeastMutationPromoterRPS2Available sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
PX458_HLF_2
Plasmid#86302PurposeEncodes gRNA for 3' target of human HLFDepositorInsertgRNA against HLF (HLF Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_HLF
Plasmid#86262PurposeDonor vector for 3' FLAG tag of human HLFDepositorInsertHLF homology arms (HLF Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_HLF_1
Plasmid#86301PurposeEncodes gRNA for 3' target of human HLFDepositorInsertgRNA against HLF (HLF Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNB788
Plasmid#60165PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by RNR1 promoter.DepositorInsertFLuc-yEVenus
UseTagsExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterRNR1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
cACTB
Plasmid#236544PurposeFor expression of ACTB in mammalian cellsDepositorInsertACTB
UseTagsExpressionMammalianMutationWTPromoterhpACTBAvailable sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
HA-cNM2C
Plasmid#236535PurposeFor expression of NM2C (dog) in mammalian cellsDepositorInsertMYH14
UseTagsHAExpressionMammalianMutationWTPromoterAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
HA-cNM2B-Flag
Plasmid#236534PurposeFor expression of NM2B (dog) - with HA and FLAG tags in mammalian cellsDepositorInsertMYH10
UseTagsFLAG and HAExpressionMammalianMutationWTPromoterAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
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iNicSnFR12
Plasmid#227142PurposeGenetically encoded fluorescent biosensor for nicotine detectionDepositorInsertiNicSnFR12
UseTags6xHis-HA and MycExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
WDV
Plasmid#226983PurposeExpresses the HUH endonuclease WDV, used as a no ligand control for DNA tension sensor experimentsDepositorInsertWDV (repA )
UseTags6xHis and SUMOExpressionBacterialMutationPromoterT7Available sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVUT-spC3GFP
Plasmid#225487PurposeTet-on lentiviral vector for controlled expression of a secretable and permeable form of C. botulinum exoenzyme C3 transferase fused with EGFPDepositorInsertSecretable and permeable exoenzyme C3 transferase
UseLentiviralTagsEGFPExpressionMammalianMutationAmino acids 30-251PromoterHuman ubiquitin CAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-HGM (optimized)
Plasmid#220988PurposeAdeno-associated viral vector to express HaloTag-GFP-mito fusion, a cytosolic-localized Halotag-GFP fusion constructDepositorInsertHaloTag-GFP-mito
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFH2.17_GagZip-MCP-dpol-P2A-eGFP
Plasmid#205532PurposeExpress HIV GagZip-MCP-dpol-P2A-eGFPDepositorInsertGag-MCP-dpol
UseTagsExpressionMammalianMutationNucleocapsid replaced with leucine zipper, pol fr…PromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH2.98_PB-EPN1-MCP_Myc-T2A-eGFP-PGK-Blast
Plasmid#205543PurposeExpress EPN1-MCP-Myc-T2A-eGFP and blasticidin resistance markerDepositorInsertEPN1-MCP-Myc
UsePiggybacTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
1BR9-AtWUS
Plasmid#193865PurposeE. coli expression vector for N-ter GFP11 and C-ter R9 tagged WUSCHEL protein from Arabidopsis thaliana.DepositorInsertAtWUS E. Coli Codon Optimized
UseTags6xHis, GFP11, and R9ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only