We narrowed to 18,942 results for: Tec

1. SPLICS Po-Mt Short P2A

   Plasmid

   #164116

   Purpose

   Detect the short Peroxisomes-mitochondria contact

   Depositor

   Marisa Brini, Tito Calì

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split GFP Po-Mt Short P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-Tight-Puro_Sox10

   Plasmid

   #46536

   Purpose

   A lentiviral vector for the expression of mouse Sox10 under doxycyline control.

   Depositor

   Paul Tesar

   Article

   Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

   Insert

   Sox10 (Sox10 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCJR01

   Plasmid

   #187888

   Purpose

   Escherichia coli plasmid with cloned regions of homology to Campylobacter jejuni chromosome flanking a NotI restriction site. pCJR01 functions as a suicide vector for integrating cloned NotI DNA.

   Depositor

   Gaylen Uhlich

   Article

   Uhlich et al Biotechniques. 2022 Apr 13. doi: 10.2144

   Insert

   DNA insert has homology to a rRNA region of the Campylobacter jejuni chromosome with an added central NotI restriction site
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   none

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. LYN11-FRB-CFP

   Plasmid

   #38003

   Depositor

   Robin Irvine

   Article

   Hammond et al Science. 2012 Jun 21.

   Inserts

   LYN (LYN Human)
   MTOR (MTOR )
   

   Use

   Tags

   ECFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

5. SPLICS Po-ER Short P2A

   Plasmid

   #164114

   Purpose

   Detect the long Peroxisomes-Endoplasmic reticulum contact

   Depositor

   Marisa Brini, Tito Calì

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split YFP Po-ER Short P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mCit-4aa-SNAP25B

   Plasmid

   #53235

   Purpose

   Fluorescent probe: SNAP-25B, N-terminally-tagged with monomeric Citrine, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCer-4aa-VAMP2 to measure FRET and dispersion.

   Depositor

   Robert Zucker

   Article

   Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

   Insert

   SNAP-25B (Snap25 Rat)
   

   Use

   Tags

   4aa-linker and mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MSGV Mouse Acceptor PGK NGFR

   Plasmid

   #128482

   Purpose

   A TCR Variable Region cloning expression vector using Mouse Constant Regions to reduce mis-pairing with endogenous TCR expressing NGFR ref Biotechniques. 2015 Mar 1;58(3):135-9

   Depositor

   David Ott

   Article

   Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   MSCV

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX458-ECFP

   Plasmid

   #112220

   Purpose

   Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and ECFP

   Depositor

   Yinming Liang

   Article

   Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

   Insert

   ECFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mCer-4aa-VAMP2

   Plasmid

   #53233

   Purpose

   Fluorescent probe: VAMP2 (synaptobrevin2), N-terminally-tagged with monomeric Cerulean, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCit-4aa-SNAP25B for FRET.

   Depositor

   Robert Zucker

   Article

   Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

   Insert

   VAMP2 (Vamp2 Rat)
   

   Use

   Tags

   4aa-linker and mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pLVX-Tight-Puro_Nkx2.2

    Plasmid

    #64840

    Purpose

    A lentiviral vector for the expression of mouse Nkx2.2 under doxycyline control.

    Depositor

    Paul Tesar

    Article

    Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

    Insert

    Nkx2.2 (Nkx2-2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEGFP-CLCa

    Plasmid

    #196912

    Purpose

    Visualization of clathrin-coated pit formation in living mammalian cells

    Depositor

    Kenji Sugimoto

    Article

    Sakaushi et al Biosci Biotechnol Biochem. 2007 Feb;71(2

    Insert

    Clathrin-Coated light chain
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pL-SFFV.Reporter.RFP657.PAC

    Plasmid

    #61395

    Purpose

    Lentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-Tight-Puro_Olig2

    Plasmid

    #64842

    Purpose

    A lentiviral vector for the expression of mouse Olig2 under doxycyline control

    Depositor

    Paul Tesar

    Article

    Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

    Insert

    Olig2 (Olig2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLVX-Tight-Puro_ST18

    Plasmid

    #64844

    Purpose

    A lentiviral vector for the expression of mouse ST18 under doxycyline control

    Depositor

    Paul Tesar

    Article

    Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

    Insert

    ST18 (St18 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mEos3.2-cortactin

    Plasmid

    #193287

    Purpose

    mammalian transient expression of mEos3.2-cortactin

    Depositor

    Klaus Hahn

    Article

    Herron et al Nat Commun. 2022 Jul 27;13(1):4363. doi:

    Insert

    cortactin (Cttn Mouse)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. VAMP2-12aa-mCit

    Plasmid

    #53237

    Purpose

    Fluorescent probe: SNAP-25B, C-terminally-tagged with monomeric Citrine, connected with a 12 amino acid linker. Expressed in mammalian cells, used with Stx1a-12aa-mCer to measure FRET and dispersion.

    Depositor

    Robert Zucker

    Article

    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

    Insert

    VAMP2 (Vamp2 Rat)
    

    Use

    Tags

    12aa-linker and mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pLVX-Tight-Puro_Nkx6.2

    Plasmid

    #64846

    Purpose

    A lentiviral vector for the expression of mouse Nkx6.2 under doxycyline control

    Depositor

    Paul Tesar

    Article

    Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

    Insert

    Nkx6.2 (Nkx6-2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLVX puro TRIM21 C54Y - GFP

    Plasmid

    #116942

    Purpose

    Expression of TRIM21 C54Y -GFP fusion

    Depositor

    Gaudenz Danuser

    Article

    Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

    Insert

    TRIM21 C54Y-GFP (TRIM21 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    C54Y

    Promoter

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGS_N_3xFLAG

    Plasmid

    #101654

    Purpose

    Expression of type I signal-peptide containing proteins with N-terminal 3xFLAG-tag in insect cells

    Depositor

    Monika Golas, Bjoern Sander

    Article

    Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

    Insert

    LRP6 Signal peptide, KpnI linker, 3xFLAG tag
    

    Use

    Tags

    3xFLAG tag

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCer-Vamp2(qv/fw)

    Plasmid

    #53238

    Purpose

    Mutant (Q76V/F77W), tetanus toxin insensitive VAMP2 (synaptobrevin2), N-terminally-tagged with mCerulean, connected by a 4 amino acid linker. For mammalian cells, used with mCit-4aa-SNAP25B for FRET.

    Depositor

    Robert Zucker

    Article

    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

    Insert

    VAMP2 (Vamp2 Rat)
    

    Use

    Tags

    4aa-linker and mCerulean

    Expression

    Mammalian

    Mutation

    Mutated Q76 to V; F77 to W.

    Promoter

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only