We narrowed to 10,582 results for: plasmids 101

1. pBTK564

   Plasmid

   #110613

   Purpose

   BTK assembled broad-host-range plasmid encoding NanoLuc luciferase protein driven by PA1 promoter

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   Nanoluc
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBTK563

   Plasmid

   #110612

   Purpose

   BTK assembled broad-host-range plasmid encoding NanoLuc luciferase protein driven by CP25 promoter

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   Nanoluc
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CAG-FlpX-mKate2.0-N2cG

   Plasmid

   #234711

   Purpose

   pAAV transfer plasmid with CAG promoter driving Flp-dependent expression of mKate2.0 and N2c G protein separated by a T2A linker

   Depositor

   David Ng

   Article

   Mendelsohn et al bioRxiv [Preprint]. 2024 Sep 18:2024.08.

   Insert

   mKate2.0 T2A N2cG
   

   Use

   AAV

   Tags

   T2A

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC-GFP-AT

   Plasmid

   #133306

   Purpose

   "Burdensome" GFP expressing plasmid carrying axe/txe toxin-antitoxin for plasmid stabilisation

   Depositor

   Chris Barnes

   Article

   Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

   Insert

   axe/txe toxin-antitoxin cassette
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. p189

   Plasmid

   #65207

   Purpose

   Mutant EGFP expression plasmid for the creation of a heteroduplex DNA repair assay plasmid with pGEM5Z(+)-EGFP (p111). *Please see notes in comments section.

   Depositor

   LuZhe Sun

   Article

   Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Premature stop codon added to pGEM5Z(+)-EGFP (TGG…

   Promoter

   CMV

   Available Since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pRR-ERalpha-5Z

   Plasmid

   #23061

   Depositor

   Charles Miller

   Article

   Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

   Insert

   ERalpha (ESR1 Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 31, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pUC-GFP-HS

   Plasmid

   #133305

   Purpose

   "Burdensome" GFP expressing plasmid carrying hok/sok toxin-antitoxin for plasmid stabilisation

   Depositor

   Chris Barnes

   Article

   Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

   Insert

   hok/sok toxin-antitoxin cassette
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBTK601

   Plasmid

   #110607

   Purpose

   BTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration)

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   Cas9
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPTG-GG-sg2+sg3

   Plasmid

   #127199

   Purpose

   Plasmid expressing sgRNA2 (TACCACATTTGTAGAGGTT) & sgRNA3 (CAATGTATCTTATCATGTC) as polycistronic tRNA-gRNA from single U6 promoter

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Insert

   tRNA-gRNA
   

   Use

   Empty sgrna plasmid

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. ADH1pro_GCN4_SIR4

    Plasmid

    #138620

    Purpose

    Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement

    Depositor

    Rob Mitra

    Insert

    GCN4 (GCN4 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available Since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAc5.1C-Fluc-CG33111_A

    Plasmid

    #145866

    Purpose

    Insect Expression of CG33111-3UTR

    Depositor

    Elisa Izaurralde

    Insert

    CG33111-3UTR (CG33111 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTS2308-Tier1-pGL3-PPGK-NLuc_TB

    Plasmid

    #169551

    Purpose

    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    pGL3-insulator-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PPGK

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSB235

    Plasmid

    #164130

    Purpose

    PhlF-RFP expression plasmid

    Depositor

    Christopher Voigt

    Article

    Shao et al Nat Commun. 2021 Mar 5;12(1):1475. doi:

    Insert

    PhlF-RFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGC4593

    Plasmid

    #110206

    Purpose

    Accessory plasmid with inducible TEV protease and unnatural tRNA system (AcF)

    Depositor

    Guillaume Cambray, Adam Arkin

    Article

    Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

    Inserts

    TEV protease
    aaRS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pbad and Ptet

    Available Since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSB233

    Plasmid

    #164128

    Purpose

    PP7-CFP and PhlF-RFP expression plasmid

    Depositor

    Christopher Voigt

    Article

    Shao et al Nat Commun. 2021 Mar 5;12(1):1475. doi:

    Insert

    PP7-CFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library 1

    Pooled Library

    #168776

    Purpose

    Pooled library expressing barcoded SARS-CoV-2 RNA Binding Domain mutations

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Expression

    Yeast

    Species

    Sars-cov-2

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

17. SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library 2

    Pooled Library

    #168777

    Purpose

    Pooled library expressing barcoded SARS-CoV-2 RNA Binding Domain mutations

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Expression

    Yeast

    Species

    Sars-cov-2

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

18. T4 Lysozyme K48-[LP] WT*

    Plasmid

    #18345

    Depositor

    Brian Matthews

    Insert

    T4 Lysozyme (e Bacteriophage T4)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    K48-[LP] WT*

    Promoter

    Available Since

    Aug. 26, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pcI-ts,ind+-(NSP8)

    Plasmid

    #160656

    Purpose

    Expresses codon optimized SARS-CoV-2 NSP8 in E. coli

    Depositor

    Kenneth Johnson

    Article

    Dangerfield et al iScience. 2020 Nov 28:101849. doi: 10.10

    Insert

    SARS-CoV-2 NSP8 (ORF1ab Synthetic)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 10, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pSB207

    Plasmid

    #164122

    Purpose

    J23101 driving PP7 repeats

    Depositor

    Christopher Voigt

    Article

    Shao et al Nat Commun. 2021 Mar 5;12(1):1475. doi:

    Insert

    YFP-PP7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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