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pCMV3flag8SHARPIN

Plasmid

#50014

Purpose

Mammalian expression of human SHARPIN with a c-terminal 3xFLAG tag, and hygromycin selection.

Depositor

Martin Dorf

Publication

Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

Insert

SHANK-associated RH domain interactor (SHARPIN Human)

Tags

3 x FLAG

Expression

Mammalian

Promoter

CMV

Available since

Dec. 31, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pMXs-IP HA-Parkin

Plasmid

#38248

Depositor

Noboru Mizushima

Publication

Yoshii et al J Biol Chem. 2011 Jun 3;286(22):19630-40

Insert

Parkin (PRKN Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pCVL SFFV-EF1s HA.NLS.Sce(opt)

Plasmid

#31479

Depositor

Andrew Scharenberg

Publication

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV/Ef1s HA NLS I-SceI

Use

Lentiviral

Tags

HA and NLS

Mutation

"opt" indicates the coding sequence has…

Available since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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hWIP1 FLAG

Plasmid

#28105

Depositor

Lawrence Donehower

Publication

Moon et al J Biol Chem. 2010 Apr 23. 285(17):12935-

Insert

WIP1 (PPM1D Human)

Tags

FLAG

Expression

Mammalian

Available since

April 12, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pAdTrack-CMV FLAG Rev-Erb alpha

Plasmid

#22745

Depositor

Bruce Spiegelman

Publication

Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

Insert

Rev Erb alpha (NR1D1 Human)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Available since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GFP-Mfn1

Plasmid

#141154

Purpose

Mammalian expression and labeling mitofusin 1

Depositor

Gia Voeltz

Publication

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

Mitofusin 1 (MFN1 Human)

Tags

AcGFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAG-SpCas9-GFP-U6-gRNA

Plasmid

#79144

Purpose

All-in-one CRISPR/Cas9 vector with CAG promoter for expression in human ESC/iPSC

Depositor

Jizhong Zou

Publication

Stoehr et al JCI Insight. 2019 May 21;5. pii: 128496.

Insert

SpCas9

Use

CRISPR

Tags

2A-GFP

Expression

Mammalian

Promoter

CAG

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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pGFP-Rab8A[Q67L]

Plasmid

#24900

Depositor

Maxence Nachury

Publication

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

hRab8A[Q67L]

Tags

GFP

Expression

Mammalian

Mutation

Changed Glutamine 67 to Leucine

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pBluescriptSKII+ U6-sgRNA(F+E) empty

Plasmid

#74707

Purpose

Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequence

Depositor

Eugene Yeo

Publication

Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

Insert

U6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence

Expression

Mammalian

Available since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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CAG-Cas9-T2A-EGFP-ires-puro

Plasmid

#78311

Purpose

Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.

Depositor

Timo Otonkoski

Publication

Saarimaki-Vire et al Cell Rep. 2017 Apr 11;19(2):281-294. doi

Insert

WT SpCas9-T2A-EGFP-ires-puromycin resistance

Use

CRISPR

Tags

T2A-EGFP-ires-puro

Expression

Mammalian

Promoter

CAG

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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GFP-MAP1B

Plasmid

#44396

Depositor

Phillip Gordon-Weeks

Publication

Scales et al J Cell Sci. 2009 Jul 15;122(Pt 14):2424-

Insert

MAP1B (Map1b Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

May 21, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pcDNA3-Myc3-CAND1

Plasmid

#19948

Depositor

Yue Xiong

Publication

Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

Insert

CAND1 (cullin-associated and neddylation-dissociated 1) (CAND1 Human)

Tags

myc3

Expression

Mammalian

Available since

April 1, 2009

Availability

Academic Institutions and Nonprofits only
anxA2-GFP

Plasmid

#107196

Purpose

Expresses human annexin A2-GFP fusion protein for live cell imaging

Depositor

Volker Gerke, Ursula Rescher

Publication

Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

Insert

annexin A2 (ANXA2 Human)

Tags

Green Fluorescent Protein

Expression

Mammalian

Mutation

Ala residue 65 in human sequence replaced by Glu …

Promoter

CMV

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLNC Wnt-3aHA

Plasmid

#18030

Depositor

Jan Kitajewski

Publication

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-3a (Wnt3a Mouse)

Use

Retroviral

Tags

HA

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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Myc-tagged delta N (dominant-negative form) human TCF4E pcDNA3

Plasmid

#32739

Depositor

Frank McCormick

Publication

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

TCF4E (Tcf7l2 Human)

Tags

Myc

Expression

Mammalian

Mutation

The N-terminal deletion form (delta N) lacks amin…

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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plenti-CAG -IRES-GFP

Plasmid

#69047

Purpose

Lentiviral backbone for GFP coexpression

Depositor

William Kaelin

Publication

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Use

Lentiviral

Tags

IRES-GFP

Expression

Mammalian

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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LcV2-Hygro

Plasmid

#91977

Purpose

LentiCRISPR v2 with Hygro cloned in place of Puro

Depositor

Joshua Mendell

Publication

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pEGFP-N1 human cofilin S3E

Plasmid

#50861

Purpose

expresses psuedo-phosporylated, non-activatable cofilin fused to EGFP in mammalian cells

Depositor

James Bamburg

Publication

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3E (CFL1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S3E psuedo-phosporylated, non-activatable

Promoter

CMV

Available since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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Cilantro 2

Plasmid

#74450

Purpose

Can clone in a C2H2 zinc finger via BsmBI restriction sites and monitor post-translational degradation using EGFP:mCherry ratio (Lentiviral, PGK.BsmBICloneSite-EGFP.IRES.mCherry.cppt.EF1a.PuroR)

Depositor

Benjamin Ebert

Publication

Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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ARR3tk-eGFP/SV40-mCherry

Plasmid

#132360

Purpose

To measure transcriptional activity of Androgen Receptor

Depositor

Charles Sawyers

Publication

Lee et al Elife. 2019 Jan 15;8. pii: 41913. doi: 1

Insert

AR responsive elements (AR Human)

Use

Lentiviral

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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