220,697 results

1. pMW37: pRS426-R2Bm RNA

   Plasmid

   #209291

   Purpose

   Contains the R2Bm non-LTR retrotransposon 5'UTR and 3'UTR sequences

   Depositor

   Feng Zhang

   Article

   Wilkinson et al Science. 2023 Apr 21;380(6642):301-308.

   Insert

   R2Bm 5'UTR and 3'UTR
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   GAL1 GAPDH

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTRE3G-Bi-gePSI-pCamk2a-AcGFP

   Plasmid

   #133732

   Purpose

   Expresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a Camk2a promoter.

   Depositor

   Erin Schuman

   Article

   Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

   Inserts

   gePSI-β-chain
   gePSI-α-chain
   AcGFP1
   

   Use

   AAV

   Tags

   murine ornithine decarboxylase - degron (ODC36)

   Expression

   Mammalian

   Mutation

   Promoter

   pCamk2a and pTRE3G-Bi

   Available since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSECRETS-A

   Plasmid

   #196986

   Purpose

   For SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).

   Depositor

   Eric Josephs

   Article

   Herring-Nicholas et al Commun Biol. 2024 Jan 12;7(1):86. doi: 1

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pltetO1

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR Src-TagBFP

   Plasmid

   #171036

   Purpose

   TagBFP-tagged Src kinase

   Depositor

   Jared Toettcher

   Article

   Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

   Insert

   Src
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGBW-m4046959

   Plasmid

   #145722

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 ORF3a protein

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 ORF3a protein (ORF3a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 27, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. p3xFLAG CMV14 / BRAF-D594G

   Plasmid

   #131719

   Purpose

   Mammalian Expression of Flag tagged BRAF

   Depositor

   Jacques De Grève

   Article

   Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

   Insert

   BRAF (BRAF Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D594G

   Promoter

   CMV

   Available since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21a AHU

   Plasmid

   #26640

   Depositor

   Phoebe Rice

   Article

   Swinger et al EMBO J. 2003 Jul 15. 22(14):3749-60.

   Insert

   AHU
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. hFLT1

   Plasmid

   #83435

   Purpose

   human VEGFR1 with HA and FLAG tag

   Depositor

   Christie Thomas

   Article

   Raikwar et al Exp Cell Res. 2013 Oct 15;319(17):2578-8

   Insert

   human VEGFR1 (FLT1 Human)
   

   Use

   Tags

   Flag and Myc

   Expression

   Mammalian

   Mutation

   HA-tag was inserted 30 AA downstream of the FLT1 …

   Promoter

   CMV

   Available since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHHSDKXK (#19)

   Plasmid

   #35964

   Depositor

   Thomas Sato

   Article

   Schlaeger et al Proc Natl Acad Sci U S A. 1997 Apr 1;94(

   Inserts

   Tie2 promoter
   SDKLacZ
   SV40 pA
   Tie2 enhancer
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 9, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pG/N795

    Plasmid

    #108220

    Purpose

    Yeast expression vector for rat glucocorticoid receptor

    Depositor

    Didier Picard, Keith Yamamoto

    Article

    Schena et al Methods Enzymol. 1991;194:389-98.

    Insert

    Rat glucocorticoid receptor
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Sept. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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