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pCAG-SRGAP1-Myc

Plasmid

#168503

Purpose

Expression of full length SRGAP1 with a C-terminal Myc fusion

Depositor

Franck Polleux

Article

Coutinho-Budd et al J Cell Sci. 2012 Jul 15;125(Pt 14):3390-

Insert

SRGAP1 (SRGAP1 Human)

Tags

Myc

Expression

Mammalian

Mutation

aa 1-501

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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perbB1-Citrine

Plasmid

#40266

Depositor

Martin Offterdinger

Article

Offterdinger et al Traffic. 2008 Jan;9(1):147-55. Epub 2007

Insert

erbB1 (EGFR Human)

Tags

Citrine

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 26, 2012

Availability

Academic Institutions and Nonprofits only

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LentiGuidPuro-hTP73_sgRNA-1

Plasmid

#88855

Purpose

CRISPR KO of Trp73

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp73

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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3789 pmko-neo RNAi-GFP

Plasmid

#8881

Depositor

Stanley Korsmeyer

Article

Wang et al EMBO J. 2005 Jan 26. 24(2):368-81.

Insert

GFP

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
TB:4xHRE_RE:MiniP

Plasmid

#178313

Purpose

4 copies of the HRE DNA binding motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

Insert

4 copies of the heat shock DNA binding motif

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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Col1a-3'probe

Plasmid

#20731

Depositor

Rudolf Jaenisch

Article

Beard et al Genesis. 2006 Jan . 44(1):23-8.

Insert

Col1a-3'probe (Col1a1 Mouse)

Expression

Bacterial

Available Since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

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pRU53

Plasmid

#167679

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::spCas9

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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GLI-3 bs-2

Plasmid

#16420

Depositor

Bert Vogelstein

Article

Kinzler et al Nature. 1988 Mar 24. 332(6162):371-4.

Insert

GLI3 (GLI3 Human)

Expression

Bacterial

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
pSP64-GFP-ePDZ-CAAX

Plasmid

#135603

Purpose

Expression of GFP-ePDZ-CAAX mRNA for microinjection to be used for optogenetics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

Lifeact-GFP-ePDZ-CAAX

Use

In vitro transcription (mrna synthesis)

Tags

CAAX, EGFP, and ePDZ

Promoter

Unknown

Available Since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET26_MraYaa

Plasmid

#100166

Purpose

recombinant expression of target protein as an MBP-His tag fusionat the N-terminus of target protein

Depositor

Seok-Yong Lee

Article

Chung et al Science. 2013 Aug 30;341(6149):1012-1016

Insert

mraY (phospho-MurNAc-pentapeptide translocase)

Tags

MBP-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-UBC-MCP-mNeonGreen

Plasmid

#207581

Purpose

The lentiviral plasmids for expression of MCP-NeonGreen

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

MS2 coat protein mNeonGreen fusion

Use

Lentiviral

Tags

mNeonGreen

Promoter

UBC

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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FRB-ECFP(W66A)-Giantin

Plasmid

#67903

Purpose

Expresses a Golgi located FRB domain in mammalian cells (the ECFP is not fluorescent)

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

FRB-ECFP(W66A)-Giantin

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pMS29: pDonor(PmcCAST)

Plasmid

#168162

Purpose

PmcCAST donor harboring non-functional cargo.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PmcCAST right end (RE)
PmcCAST left end (LE)

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pEF-TAZ-N-Flag

Plasmid

#19025

Depositor

Michael Yaffe

Article

Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

Insert

TAZ (Wwtr1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Vector has no SV40 origin

Available Since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only
pTE101

Plasmid

#59396

Purpose

MethyloBrick cloning vector, no promoter; KmR

Depositor

Tobias Erb

Article

Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

none

Available Since

Dec. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-hIFITM3

Plasmid

#58461

Purpose

Expresses human IFITM3 with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (IFITM3 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV IE

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pFSynWSyt1C2AB(80-421)msGFP

Plasmid

#209014

Purpose

Overexpression of truncated syt1 tagged with GFP in mammalian cells

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Use

Lentiviral

Tags

msfGFP

Expression

Mammalian

Mutation

aa 80-421 only

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P33

Plasmid

#120018

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with FLAG

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

FLAG

Use

Pcr template

Tags

FLAG

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z34

Plasmid

#120062

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 3xFLAG

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xFLAG

Use

Pcr template

Tags

3xFLAG

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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TAL2430

Plasmid

#36829

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

HDAC2-TALEN_Left (Hdac2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only