We narrowed to 24,380 results for: LEA

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pET30a-YedK

Plasmid

#86884

Purpose

Bacterial expression of E. coli SRAP ortholog YedK

Depositor

Douglas Feldman

Publication

Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

Insert

YedK (yedK Eschericia coli)

Tags

6xHis, HA tag, and S-Tag

Expression

Bacterial

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pαH-S-GSAS-OMICRON.BA.2

Plasmid

#184829

Purpose

Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON.BA.2 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

Depositor

Priyamvada Acharya

Publication

Stalls et al Cell Rep. 2022 Jun 28;39(13):111009. doi

Insert

S-GSAS-OMICRON.BA.2 (S Severe acute respiratory syndrome coronavirus 2)

Tags

HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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FUGW-VMAT2-pHluorin

Plasmid

#207468

Purpose

Lentiviral expression of VMAT2-pHluorin

Depositor

Robert Edwards

Publication

Silm et al Neuron. 2019 May 22;102(4):786-800.e5. d

Insert

VMAT2 (Slc18a2 Rat)

Use

Lentiviral

Tags

pHluorin

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLVH-EF1A-BRD3R

Plasmid

#130696

Purpose

Lentiviral plasmids encoding human BRD3R

Depositor

Kejin Hu

Publication

Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

Insert

Human BRD3R (BRD3 Human)

Use

Lentiviral

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p3E_he1a:ECFP

Plasmid

#113880

Purpose

p3E entry vector for Three Multisite Gateway

Depositor

Marnie Halpern

Insert

he1a (he1.1 Zebrafish)

Use

Zebrafish danio expression

Tags

ECFP

Available since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pGEXNKI-GST-3C-LIC

Plasmid

#108710

Purpose

Expression of a GST-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Publication

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Tags

GST-3C protease cleavage site

Expression

Bacterial

Promoter

tac

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV2-SBECN1W

Plasmid

#107937

Purpose

Adeno-Associated viral (AAV) vector plasmid expressing mouse Beclin-1 (BECN1) in neurons

Depositor

Johan Jakobsson

Publication

Pircs et al Cell Rep. 2018 Aug 7;24(6):1397-1406. do

Insert

mouse Beclin-1 (BECN1) (Becn1 Mouse)

Use

AAV

Promoter

hSynapsin

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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p3-K230-FokI_R (wt)

Plasmid

#32545

Depositor

Jin-Soo Kim

Publication

Lee et al Genome Res. 2010 Jan;20(1):81-9. Epub 20

Insert

ZFN

Tags

HA epitope

Expression

Mammalian

Available since

Dec. 2, 2011

Availability

Academic Institutions and Nonprofits only
pJL057

Plasmid

#177169

Purpose

Expresses a nuclease dead MRE11 in human cells

Depositor

Brittany Adamson

Publication

Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

Insert

MRE11 H129N (MRE11 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

nuclease-inactivating mutation (H129N)

Promoter

EF1a

Available since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pSin-GFP-Fg

Plasmid

#174307

Purpose

Lentiviral expression of GFP

Depositor

Bruce Beutler

Publication

Yue et al Science. 2021 May 14;372(6543). pii: 372

Insert

GFP (eGFP Aequorea victoria)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

EF1a

Available since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBad-UGAcS

Plasmid

#172137

Purpose

bacteria expression plasmid for a UDP-GlcNAc sensor

Depositor

Huiwang Ai

Publication

Li et al ACS Cent Sci. 2021 Oct 27;7(10):1763-177

Insert

UGAcS

Expression

Bacterial

Mutation

H605Q- please see depositor comments

Promoter

araBAD

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pBT340_pattB-pCA-GFP-pA-FRT5-pPGK-Flpo-pA

Plasmid

#52549

Purpose

transgenic construct for expression of GFP and Flpo

Depositor

Liqun Luo

Publication

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP
Flpo recombinase

Expression

Mammalian

Available since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET MBP mCitrine LIC cloning vector (MBP-mCitrine)

Plasmid

#29749

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, AviTag (biotin), MBP, TEV cleavage site, a…

Expression

Bacterial

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

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mini-donor - NKX6.1-nEGFP

Plasmid

#206047

Purpose

this mini-donor knockin vector can be used to knock nuclear EGFP into NKX6.1 locus

Depositor

Xiaojun Lian

Publication

Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

nuclear EGFP

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSpCas9nucl-T2A-EGFP - BAXgRNA1- BAXgRNA2

Plasmid

#167296

Purpose

Plasmid encoding for 2 gRNAs targeting the human BAX gene and a CMV driven nuclease Cas9 followed by self-cleaving EGFP

Depositor

Agnel Sfeir

Publication

Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

Insert

BAX (BAX Human)

Expression

Mammalian

Available since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJY3948

Plasmid

#113338

Purpose

6XHis-tagged 2-pyrone synthase driven by TEF1intron promoter; codon optimized for Yarrowia lipolytica expression

Depositor

Suzanne Sandmeyer

Publication

Yu et al Biotechnol Bioeng. 2018 May 19. doi: 10.

Insert

2-pyrone synthase, codon optimized for Y. lipolytica

Tags

6XHis

Expression

Yeast

Mutation

Please see depositor comments

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Met1 diUb (1-152)

Plasmid

#110766

Purpose

Bacterial expression of Met1 diUb

Depositor

David Komander

Publication

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

UBB (UBB Human)

Tags

N-His6-3C

Expression

Bacterial

Promoter

T7

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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TR-Csy4-H29A

Plasmid

#80602

Purpose

Expresses Csy4 with an inactivating H29A mutation from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Publication

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

Csy4-H29A

Use

AAV

Expression

Mammalian

Mutation

His29 mutated to Ala

Promoter

CBA

Available since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSYC-102

Plasmid

#74790

Purpose

pCS4+-NLS-EGFP-T2A-mCherry-CAAX

Depositor

Seok-Yong Choi

Publication

Kim et al PLoS One. 2011 . 6(4):e18556.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NLS-EGFP-T2A-mCherry-CAAX

Available since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Reb_81 aka PAS622

Plasmid

#71227

Purpose

Reb_1 with RebB(T28A, D72G)

Depositor

Pamela Silver

Publication

Polka et al ACS Synth Biol. 2016 Feb 3.

Insert

Reb locus

Tags

none

Expression

Bacterial

Mutation

RebB(T28A, D72G), RebA(G53A)

Available since

April 7, 2016

Availability

Academic Institutions and Nonprofits only