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pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut_R

Plasmid

#147388

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut (gw Fly, Nematode)

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-P-GLmut_R

Plasmid

#147390

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_635-1384-P-GLmut

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_635-1384-P-GLmut (gw Nematode, Human)

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-P-GLmut-F961A_R

Plasmid

#147391

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_635-1384-P-GLmut-F961A

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_635-1384-P-GLmut-F961A (gw Fly)

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-CIM1-2mut-F1359A-siRNAres_R

Plasmid

#147411

Purpose

Insect Expression of HsTNRC6A-CIM1-2mut-F1359A-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A-CIM1-2mut-F1359A-siRNAres (TNRC6A Human)

Expression

Insect

Mutation

one non silent mutation K1578E compared to the se…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_1-634-dsRNAres_R

Plasmid

#147382

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_1-634-dsRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_1-634-dsRNAres (gw Fly, Nematode)

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_205-490-dsRNAres_R

Plasmid

#147383

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_205-490-dsRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_205-490-dsRNAres (gw Fly, Nematode)

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-CIM1-mut_R

Plasmid

#147385

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_635-1384-CIM1-mut

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_635-1384-CIM1-mut (gw Fly, Nematode)

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-delLN-DmPABPC1-V5His6_H

Plasmid

#146477

Purpose

Insect Expression of DmPABPC1

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmPABPC1 (pAbp Fly)

Expression

Insect

Mutation

one non silent mutation G26S, and seven silent mu…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-G2BRgp78

Plasmid

#185344

Purpose

Expression of soluble G2BR, amino acids 575-600 of human gp78

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

gp78 aa 574-600 (G2BR) (AMFR Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa 575-600 only

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pETDUET-GST-AUP1 379-410:Ube2G2

Plasmid

#185335

Purpose

Used for co-expression of the AUP1 G2BR with UBE2G2 for crystallography

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 aa 379-410, Ube2G2 (AUP1 Mouse, Human)

Tags

GST

Expression

Bacterial

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pC1-ND-Cdt1-mCitrine

Plasmid

#193771

Purpose

Human Cdt1 (SV40 NLS localization and C-terminal mCitrine tag) with mutations which abroggate its S phase degradation

Depositor

Tobias Meyer

Article

Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

Insert

ND-Cdt1-mCitrine (CDT1 Human)

Tags

mCitrine

Expression

Mammalian

Mutation

amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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W118-1_hZBTB7B_R363L

Plasmid

#180381

Purpose

lentiviral transduction of human ZBTB7B gene with a point mutation (R363L) in the first ZF domain

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

ZBTB7B_R363L (ZBTB7B Human)

Use

Lentiviral

Expression

Mammalian

Mutation

R363L

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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W118-1_hZBTB7B_D35N_R49Q

Plasmid

#180383

Purpose

lentiviral transduction of human ZBTB7B gene with point mutations (D35N and R49Q) in the BTB domain

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

ZBTB7B_D35N/R49Q (ZBTB7B Human)

Use

Lentiviral

Expression

Mammalian

Mutation

D35N/R49Q

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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W118-1_hZBTB7B_R49H

Plasmid

#180382

Purpose

lentiviral transduction of human ZBTB7B gene with a point mutation (R49H) in the BTB domain

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

ZBTB7B_R49H (ZBTB7B Human)

Use

Lentiviral

Expression

Mammalian

Mutation

R49H

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shIrak1-2

Plasmid

#180398

Purpose

Producing AAV that encodes mouse Irak1 shRNA-2 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shIrak1-2 (Irak1 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shIrak1-3

Plasmid

#180399

Purpose

Producing AAV that encodes mouse Irak1 shRNA-3 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shIrak1-3 (Irak1 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shIrak1-1

Plasmid

#180397

Purpose

Producing AAV that encodes mouse Irak1 shRNA-1 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shIrak1-1 (Irak1 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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IL2-mCherry-rGBD in pmCherryN1

Plasmid

#187286

Purpose

Express IL2-Rhotekin GTPase-binding domain-mCherry

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

interleukin-2 receptor alpha-subunit, Rhotekin (IL2, Rtkn Mouse, Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pPBpuro-EGFP-eDHFR(69K6)-cRaf

Plasmid

#178849

Purpose

Mammalian expression of cRaf fused to EGFP-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

EGFP-eDHFR(69K6)-cRaf (RAF1 Human)

Expression

Mammalian

Promoter

CAG

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pPBpuro-cRaf-mNG-eDHFR(69K6)

Plasmid

#172107

Purpose

Mammalian expression of cRaf fused to mNeonGreen-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

cRaf-mNeonGreen-eDHFR(69K6) (RAF1 Human)

Expression

Mammalian

Promoter

CAG

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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