167,642 results

1. C1(1-29)-TurboID-V5_pCDNA3

   Plasmid

   #107173

   Purpose

   expresses V5-tagged TurboID on the mammalian ER membrane

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Expression

   Mammalian

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV9)

   Viral Prep

   #112677-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Promoter

   EF1a

   Tags

   mCherry (Cre-negative cells), EGFP (Cre-positive cells)

   Available Since

   Sept. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

3. pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 (AAV Retrograde)

   Viral Prep

   #105551-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 (#105551). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 plasmid DNA. Expression of GFP-Cre from CamKII promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   CaMKII

   Tags

   GFP

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

4. pORI28

   Plasmid

   #71595

   Purpose

   EC1000 containing pORI28. Used as the source of plasmid pORI28. With pTRK669, part of a system for chromosomal integration in gram positive bacteria.

   Depositor

   Todd Klaenhammer, Jan Kok

   Article

   Leenhouts et al Mol Gen Genet. 1996 Nov 27;253(1-2):217-

   Type

   Empty backbone

   Use

   Integration vector for bacteria

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLG005 (Retron-TIR)

   Plasmid

   #157883

   Purpose

   Expresses the retron-TIR defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   Retron-TIR
   

   Expression

   Bacterial

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-Calreticulin-N-16

   Plasmid

   #55006

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Calreticulin (CALR Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBS_nanorod_105b

   Plasmid

   #120347

   Purpose

   phagemid containing all components for assembly of a DNA origami nanorod (10-helix bundle)

   Depositor

   Hendrik Dietz

   Article

   Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

   Insert

   Staple sequences for a DNA origami nanorod
   

   Expression

   Bacterial

   Promoter

   none

   Available Since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-dCas9

   Plasmid

   #47106

   Purpose

   Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Flag and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive)

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDESTpK7WG2mito

   Plasmid

   #158726

   Purpose

   Plant mitoTALEN vector assembly, Destination vector

   Depositor

   Shin-ichi Arimura

   Article

   Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

   Insert

   mitochondrial presequence in front of attR1 in pk7WG2
   

   Expression

   Plant

   Available Since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKT2/CAGXSP/CD63mScarlet

    Plasmid

    #182972

    Purpose

    Stably express CD63 fused with mScarlet in mammalian cells via the sleeping beauty transposon system.

    Depositor

    Masamitsu Kanada

    Article

    Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

    Insert

    CD63-mScarlet (CD63 Human)
    

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-JEDI-2P-WPRE

    Plasmid

    #179464

    Purpose

    Genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for neuron -specific expression using the promoter hSyn

    Depositor

    Francois St-Pierre

    Article

    Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

    Has Service

    AAV9

    Insert

    JEDI-2P
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. mApple-N1

    Plasmid

    #54567

    Purpose

    Localization: N1 Cloning Vector, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Type

    Empty backbone

    Tags

    mApple

    Expression

    Mammalian

    Available Since

    July 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRedF

    Plasmid

    #118057

    Purpose

    GBPart - RedF Luciferase CDS

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    Red Firefly Luciferase CDS
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Mutation

    Red mutant S286Y

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. TFORF3164

    Plasmid

    #144640

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    RUVBL1 (RUVBL1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBAD-Lime

    Plasmid

    #191255

    Purpose

    Bacterial expression plasmids for pH sensitive fluorescent protein Lime

    Depositor

    Robert Campbell

    Article

    Shen et al ACS Sens. 2023 Aug 25;8(8):3014-3022. do

    Insert

    Lime
    

    Tags

    His tag

    Expression

    Bacterial

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-HA-hIFITM3

    Plasmid

    #58397

    Purpose

    Expressed human IFITM3 with an N-terminal HA tag in mammalian cells

    Depositor

    Jacob Yount, Howard Hang

    Article

    Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

    Insert

    IFITM3 (IFITM3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV IE

    Available Since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. HA GSK3 beta K85A pcDNA3

    Plasmid

    #14755

    Depositor

    Jim Woodgett

    Article

    Hughes et al EMBO J. 1993 Feb . 12(2):803-8.

    Insert

    GSK3 beta K85A (GSK3B Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K85A. Kinase dead mutant.

    Available Since

    April 12, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. c-Jun

    Plasmid

    #187902

    Purpose

    Expresses c-Jun in transfected cells

    Depositor

    Jennifer Mitchell

    Article

    Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

    Insert

    c-Jun (JUN Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-C2_SEPT9 i4

    Plasmid

    #71617

    Purpose

    mammalian expression of human SEPT9 i4 with a GFP fusion

    Depositor

    Elias Spiliotis, Cristina Montagna

    Article

    Connolly et al Breast Cancer Res. 2011 Aug 10;13(4):R76

    Insert

    Septin 9 (SEPTIN9 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLX304_zeo_empty

    Plasmid

    #160093

    Purpose

    Control lentiviral vector for mammalian gene expression

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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