We narrowed to 19,702 results for: CAL
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Plasmid#223411PurposeT-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants select.DepositorInsert2x35s-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT19
Plasmid#223391PurposeT-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NG or NA PAM ; wide working window; A3A/Y130F-CBE_V01 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter.DepositorInsertAtUBQ10-hA3A-Y130F-SpRYD10A-UGI-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT13
Plasmid#223385PurposeT-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter.DepositorInsertAtUBQ10-hA3A-Y130F-SpCas9-D10A-UGI-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMVtrunc Affimer6-msfGFPΔN12
Plasmid#231550PurposeMammalian expression of actin-binding Affimer6 fused to N-terminally truncated monomeric superfolder GFP, for actin filament organization measurements using fluorescence polarization microscopyDepositorInsertAffimer6
TagsmsfGFPExpressionMammalianPromoterCMVtruncAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
CIRTS-control
Plasmid#213788PurposeCIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and scramble control guide RNA is expressed under human Syn1 and U6 promoter, respectively.DepositorInsertCIRTS-Calm3-U6-control gRNA
UseLentiviralTagsGFPExpressionMammalianPromoterSyn1, U6Available SinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRDA_122
Plasmid#208095PurposePerturb-Seq lentiviral gRNA expression vector with a 3' appended capture sequence to enable direct capture of CRISPR gRNAs for scRNA-seqDepositorInsertPuromycin resistance
UseCRISPR and LentiviralPromoterEF1aAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2-elavl3-WHaloCaMP1a-EGFP
Plasmid#205311PurposeExpression of WHaloCaMP1a-EGFP in zebrafish from the elavl3 promoter (pan-neuronal expression)DepositorInsertWHaloCaMP1a-EGFP
UseZebrafish expressionAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CamKIIa-moxBFP-IRES-Flpo
Plasmid#194977PurposeAAV vector to drive expression of moxBFP and Flpo under the control of CamKIIalpha promoterDepositorInsertmoxBFP, Flpo
UseAAVAvailable SinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-LSSA12
Plasmid#186546PurposeLSSA12 fluorescent protein (ex/em = 398/511 nm): bacterial expression plasmidDepositorInsertLSSA12
Tags6xHisExpressionBacterialMutationhfYFP-G65S/Y203I/Q204E/E222D/R223FPromoteraraBAvailable SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_Ollas.S.V5_mCherry-NLS
Plasmid#178211PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsNuclear Localization Signal and Ollas.S.V5PromotereF1aAvailable SinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-sypHTomato
Plasmid#164613PurposeExpress the pHTomato inserted in the synaptophysion in mammalian cellsDepositorInsertA reporter of vesicular turnover sypHTomato
ExpressionMammalianPromoterCMVAvailable SinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_KPNB1_HEAT
Plasmid#110017PurposeProtein expression and purification of KPNB1_HEATDepositorInsertKPNB1_HEAT (KPNB1 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp2-GFPuv
Plasmid#139083PurposeLow phosphate inducible promoter yibDp2 for GFP expressionDepositorInsertyibDp2-GFPuv
ExpressionBacterialAvailable SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
OSCA1.8
Plasmid#136594PurposeExpresses the mechanosensitive channel OSCA1.8 in mammalian cellsDepositorAvailable SinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
OSCA4.1
Plasmid#136597PurposeExpresses the mechanosensitive channel OSCA4.1 in mammalian cellsDepositorAvailable SinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBW2065_pCAG-PV1-GID1-L1-CTD-ENTRY-BGHpA
Plasmid#108843PurposeInsert protein fragment into KpnI/EcoRI to fuse to GID1 tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsGID1ExpressionMammalianAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2067_pCAG-PV1-GAI-L1-CTD-ENTRY-BGHpA
Plasmid#108845PurposeInsert protein fragment into KpnI/EcoRI to fuse to GAI tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsGAIExpressionMammalianAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-BE3-2NLS
Plasmid#119031PurposeStably express BE3 in mammalian cellsDepositorInsertBE3
Tags3xFlagExpressionMammalianAvailable SinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHLK
Plasmid#64182Purposeharbours marker genes HIS3, LEU2 and LYS2DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHUK
Plasmid#64183Purposeharbours marker genes HIS3, URA3 and LYS2DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only