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pAS19

Plasmid

#158788

Purpose

Ribosome-tagged GCaMP (soma localized) expression in C. elegans ASH neurons

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

psra-6::GCaMP6m::rpl-1a

Expression

Worm

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAS16

Plasmid

#158786

Purpose

Ribosome-tagged GCaMP (soma localized) expression in C. elegans AFD neurons (and others)

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

Pntc-1::GCaMP6m::rpl-1a

Expression

Worm

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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M26I DJ-1 TEV site pET15b

Plasmid

#60685

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

M26I DJ-1 (PARK7 Human)

Tags

6xHis

Expression

Bacterial

Mutation

M26I and TEV site was inserted (see comments belo…

Promoter

T7

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
M26L DJ-1 TEV site pET15b

Plasmid

#60686

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

M26L DJ-1 (PARK7 Human)

Tags

6xHis

Expression

Bacterial

Mutation

M26L and TEV site was inserted (see comments belo…

Promoter

T7

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
YEE-NG-CBE

Plasmid

#227418

Purpose

C-to-T base editing in mammalian cells

Depositor

Yang Sun

Article

Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

Insert

rA1-YEE-nSpCas9-NG-2xUGI (AICDA Human)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pDON223 Spike D138Y

Plasmid

#180056

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike D138Y (S SARS-CoV-2)

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike D138Y

Available Since

Jan. 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON223 Spike A570D

Plasmid

#180064

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike A570D (S SARS-CoV-2)

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike A570D

Available Since

Jan. 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON223 Spike H655Y

Plasmid

#180059

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike H655Y (S SARS-CoV-2)

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike H655Y

Available Since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON223 Spike S982A

Plasmid

#180067

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike S982A (S SARS-CoV-2)

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike S982A

Available Since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON223 Spike T1027I

Plasmid

#180058

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike T1027I (S SARS-CoV-2)

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike T1027I

Available Since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON223 Spike D1118H

Plasmid

#180068

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike D1118H (S SARS-CoV-2)

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike D1118H

Available Since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON223 Spike K417N

Plasmid

#180060

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike K417N (S SARS-CoV-2)

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike K417N

Available Since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON223 Spike A701V

Plasmid

#180062

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike A701V (S SARS-CoV-2)

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike A701V

Available Since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pHJ4

Plasmid

#158785

Purpose

Pan-neuronal nuclear-localized GCaMP expression in C. elegans

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

Prab-3::nls::GCaMP6m

Tags

NLS

Expression

Worm

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-loxSTOPlox-LynEGFP

Plasmid

#133922

Purpose

Reporter expressing membrane targeted EGFP for visualization of neuronal morphology upon Cre-based recombination

Depositor

Frank Bradke, Sebastián Dupraz

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

LynEGFP

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pHEN2-SG4

Plasmid

#196071

Purpose

Express wild type SG4 in E.coli

Depositor

Shankar Balasubramanian

Article

Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

Insert

SG4

Tags

3XFLAG and 6XHis

Expression

Bacterial

Promoter

T7

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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SpyTag003-sfGFP

Plasmid

#133454

Purpose

Expresses SpyTag003-superfolder GFP in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-sfGFP

Tags

GSGSGS linker and His6

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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SpyCatcher003

Plasmid

#133447

Purpose

Expresses SpyCatcher003 in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyCatcher003

Tags

GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#113341

Purpose

Expresses VP64-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

Depositor

Gavin Wright

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

VP64-dCas9-VP64-T2A-MS2-p65-HSF1

Use

Piggybac

Expression

Mammalian

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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LV-RFP

Plasmid

#26001

Depositor

Elaine Fuchs

Article

Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

Insert

H2B-RFP (H2BC21 Human, A. victoria)

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 10, 2010

Availability

Academic Institutions and Nonprofits only

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