We narrowed to 59,588 results for: ran

1. pcDNA3.Flag.HERC2-HECT-WT.6xHis

   Plasmid

   #39227

   Depositor

   Aziz Sancar

   Article

   Kang et al Nucleic Acids Res. 2011 Apr;39(8):3176-8

   Insert

   HERC2 HECT Domain (HERC2 Human)
   

   Tags

   6xHis and Flag

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pAAV-EnhancedSynapsin-DIO-TVA950-EYFP-WPRE

   Plasmid

   #120269

   Purpose

   Allows for initial cellular transduction of EnvA-pseudotyped Rb via Cre-dependent expression of an avian TVA receptor fused with yellow fluorescent protein

   Depositor

   Edward Callaway

   Article

   Faget et al Cell Rep. 2016 Jun 21;15(12):2796-808. d

   Insert

   eSyn-DIO-TVA950:YFP
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEYFP-hDF-C2A

   Plasmid

   #72582

   Purpose

   human dysferlin C2A domain tagged with YFP

   Depositor

   Renzhi Han

   Article

   Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

   Insert

   dysferlin C2A domain (DYSF Human)
   

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   C2A domain

   Promoter

   CMV

   Available Since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. L1dRSLE

   Plasmid

   #13266

   Depositor

   Vance Lemmon

   Article

   Cheng et al J Neurosci. 2005 Jan 12. 25(2):395-403.

   Insert

   L1CAM (L1CAM Human)
   

   Expression

   Bacterial

   Mutation

   RSLE region removed

   Available Since

   Dec. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

5. tsPurple chromoprotein promoter-less

   Plasmid

   #118007

   Purpose

   For course in "Synthetic Biology: A Lab Manual"

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   RBS, tsPurple
   

   Use

   Synthetic Biology; Escherichia coli

   Mutation

   BioBrick sites removed

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28b-ETV6-ETS

   Plasmid

   #111180

   Purpose

   Minimal ETS domain of human ETV6 (TEL)

   Depositor

   Gregory Poon

   Article

   Vo et al J Biol Chem. 2017 Aug 11;292(32):13187-1

   Insert

   ETV6 ETS domain (ETV6 Human, Bovine, Mouse)
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSET a MatryoshCaMP6s

   Plasmid

   #100019

   Purpose

   Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   MatryoshCaMP6s
   

   Tags

   6x HIS tag and Xpress_EK tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR-mCherry-attP(C31)-*BleoR

   Plasmid

   #183752

   Purpose

   phiC31 landing pad cassette.

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Insert

   FRT-mCherry-attP-BleoR-F3
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMuLE ENTR 7SK-miR-30 L1-L4

   Plasmid

   #62180

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Type

   Empty backbone

   Use

   RNAi; Mule gateway entry vector

   Expression

   Mammalian

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pSL0374 (His-MBP-Cas8_Cas7_Cas6)

    Plasmid

    #130639

    Purpose

    Expresses V. cholerae CAST Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on Cas8 for purification.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchCas8, VchCas7, VchCas6
    

    Use

    CRISPR; Transposon

    Tags

    His10-MBP-TEVsite on Cas8

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDGB1alpha2_SulR

    Plasmid

    #186425

    Purpose

    transcriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoter

    Depositor

    Lukas Fischer

    Article

    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

    Insert

    SulR
    

    Use

    Synthetic Biology; Binary vector for escherichia …

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. mRuby2-H2B-C-10

    Plasmid

    #55898

    Purpose

    Localization: Nucleus/Histones, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    H2B (H2BC11 Human)
    

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available Since

    March 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pMS1: pHelper(AvCAST)_AvPSP1

    Plasmid

    #168133

    Purpose

    Inducible expression of AvCAST proteins with crRNA targeting AvPSP1.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    AvCAST minimal CRISPR array (with spacer for AvPSP1)
    AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
    AvTnsD
    

    Expression

    Bacterial

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAC5-dual-dCas9VP48-sgTetO

    Plasmid

    #48237

    Purpose

    Dual expression construct expressing both dCas9VP48 and sgTetO from separate promoters

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9VP48 and sgTetO
    

    Use

    CRISPR

    Tags

    HA-tag and VP48

    Expression

    Mammalian

    Mutation

    D10A H840A (catalytically inactive)

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMuLE ENTR CMV Luc2 (FF-Luciferase) L5-L2

    Plasmid

    #62168

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and firefly luciferase module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    Luciferase
    

    Use

    Luciferase; Mule gateway entry vector

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET15b-eGO-Matryoshka-LE-LP

    Plasmid

    #100017

    Purpose

    Bacterial expression of fluorescent protein casette - cpEGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    cpEGFP-LSSmOrange
    

    Tags

    6x HIS tag

    Expression

    Bacterial

    Mutation

    Leu and Glu N-term linker and Leu and Pro C-term …

    Promoter

    T7

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLINE1-scFvFc

    Plasmid

    #206020

    Purpose

    An intact type LINE-1 vector plasmid possessing the scFv-Fc expression unit

    Depositor

    Masamichi Kamihira

    Article

    Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

    Insert

    mouse LINE1 vector harboring an scFv-Fc expression unit
    

    Expression

    Mammalian

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFL-EGFP-3xFlag-Fml1

    Plasmid

    #193058

    Purpose

    Baculovirus expression of S.pombe Fml1 for recombinant protein production

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Insert

    Fml1 (fml1 Fission Yeast)
    

    Tags

    3xFlag

    Expression

    Insect

    Promoter

    polyhedron

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6-sgGAL4-4

    Plasmid

    #46916

    Purpose

    Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter (negative control)

    Depositor

    Stanley Qi, Jonathan Weissman

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Inserts

    sgGAL4-4
    Puromycin resistance and mCherry
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. DNM2 delta PRD

    Plasmid

    #168275

    Purpose

    Express PRD-deleted DNM2 mutant (aa 1-742) in pEGFP-N1 vector.

    Depositor

    Cheng-han Yu

    Article

    Cao et al Commun Biol. 2020 Mar 13;3(1):117. doi:

    Insert

    DNM2 (DNM2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    aa 1-742

    Available Since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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