We narrowed to 13,756 results for: URE
-
Plasmid#114057Purposetransfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purificationDepositorAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only
-
NET23 pEGFP-N2 (1174)
Plasmid#62037Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable SinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
M26I DJ-1 TEV site pET15b
Plasmid#60685PurposeRecombinant protein expression of Park7DepositorInsertM26I DJ-1 (PARK7 Human)
Tags6xHisExpressionBacterialMutationM26I and TEV site was inserted (see comments belo…PromoterT7Available SinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
M26L DJ-1 TEV site pET15b
Plasmid#60686PurposeRecombinant protein expression of Park7DepositorInsertM26L DJ-1 (PARK7 Human)
Tags6xHisExpressionBacterialMutationM26L and TEV site was inserted (see comments belo…PromoterT7Available SinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
slpr genomic fragment
Plasmid#42074DepositorAvailable SinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
Flag-TRIM24-C840W shRescue
Plasmid#28137DepositorInsertTRIM24 (TRIM24 Human)
TagsFLAGX2ExpressionMammalianMutationC840Wmutated at aa 308 and 309 for shRNA rescue; …Available SinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-1A32
Plasmid#191241PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
Tagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailable SinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-1A31
Plasmid#191242PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
Tagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailable SinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
35S::Cel1SP-GeNL/pCAMBIA1301
Plasmid#190069PurposeExpressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana apoplastDepositorInsertCel1 signal peptide-Green-enhanced Nano-lantern
ExpressionPlantPromoterCaMV 35SAvailable SinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD+7R+12D
Plasmid#178678PurposeBacterial expression of N-terminally 6His tagged A1-LCD with seven Arg residues added, twelve Glu residues added (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD+7R+12D (HNRNPA1 Human)
Tags6xHisExpressionBacterialMutationseven Arg residues added, twelve Glu residues add…Available SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-HS-NLRC4Δcard
Plasmid#177276PurposePurification of mouse NLRC4 (90-1024) from insect cellsDepositorInsertNLRC4 (Nlrc4 Mouse)
TagsHIS-SUMO-PreScissionExpressionInsectMutationI586T, N761S, M885V, K915R and F972L- please see …PromoterpolyhedrinAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Venus-Bik-pEGFP-C1
Plasmid#166737PurposeExpress Venus fused to the N-terminus of the Bcl-2 family protein, BikDepositorAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVD1 tRNA-gRNA position E3 (GB2240)
Plasmid#160562PurposetRNA and scaffold for the assembly of GBoligomers for the third position (positon [D3_4]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoterDepositorInserttRNA-gRNA position E3 (Multiplexing Edit)
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAD96_hIRG1_4-461_pvp008
Plasmid#124874PurposeExpresses hCAD Asn152Ser mutant in E.coliDepositorInsertcis-aconitate decarboxylase (ACOD1 Human)
TagsStrepTag and TEV siteExpressionBacterialMutationmutated Asn152 to Ser, deleted amino acids 1-3 an…PromoterT7Available SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_Ab-PiuA
Plasmid#128957Purposeexpresses Acinetobacter baumannii PiuA in E. coliDepositorInsertTonB-dependent siderophore receptor
TagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removedPromoterT7Available SinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
Rescue Septin 11 mRNA
Plasmid#122025PurposeRescue mRNA for Septin 11 Knock Down with sh2 shRNA. Splice variant II (EU711415) with 5 silent mutations at SEPT11 sh2 shRNA target site.DepositorInsertSEPT11 (Sept11 Rat)
ExpressionMammalianMutationSilent mutations are in 5 consecutive codons (CUG…Available SinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28b – E. coli RecE residues 564-868
Plasmid#104534PurposeExpresses E. coli RecE residues 564-868DepositorInsertRecE residues 564-866 (C-terminal nuclease domain)
Tags6X-His tag followed by thrombin siteExpressionBacterialPromoterT7Available SinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMT mir-1-1 reporter cel-mir-40
Plasmid#46739DepositorInsertcel-mir-40
UseRNAiTagsHis and hsa-mir-1-1ExpressionInsectPromoterMetallothionein (MT)Available SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
SRAcode_in_pUC57_v3(1000-1356)
Plasmid#49827Purposefor transcribing SRA nucl 1000-1356 of NM_001035235 with T7DepositorInsertsteroid receptor RNA activator RNA coding (SRA1 Human)
ExpressionBacterialMutationKpnI site at 5' start of RNA coding seqPromoterT7Available SinceFeb. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsynapsin-DIO-adra2a-shRNA-mCherry
Plasmid#245063PurposeAAV-transgene knocking down adra2a receptor (adrenergic 2a receptor) transcripts cell type-selectively. Using the pPRIME. system, this generates mir30-derived shRNAs and a marker from the same RNA.DepositorInsertAAV-human synapsin promoter-DIO-adra2a-shRNA-mCherry (Adra2a Mouse, Synthetic)
UseAAV, Cre/Lox, and RNAiPromoterhuman synapsinAvailable SinceOct. 15, 2025AvailabilityAcademic Institutions and Nonprofits only