We narrowed to 22,513 results for: gan

1. pHES941

   Plasmid

   #87942

   Purpose

   Constitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Gal4 binding sites.

   Depositor

   Hana El-Samad

   Article

   Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

   Insert

   GAL4 DBD - hPR LBD - MSN2 AD (PGR Human, Budding Yeast, Synthetic)
   

   Expression

   Yeast

   Available Since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET21-pelB-LaG-21

   Plasmid

   #172749

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-21; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-21
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET21-pelB-LaG-37

   Plasmid

   #172756

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-37; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-37
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TetO-FUW-mir124a

   Plasmid

   #61539

   Purpose

   Expresses mir124a in mammalian cells

   Depositor

   Rudolf Jaenisch

   Article

   Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

   Insert

   mir124a (MIR124-1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

5. DNA-CARzeta-IRESpuro

   Plasmid

   #89345

   Purpose

   2nd generation lentiviral vector which expresses DNA-CAR receptor upstream of a IRES-puro cassette

   Depositor

   Ron Vale

   Article

   Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

   Insert

   DNA-CARzeta
   

   Use

   Lentiviral

   Tags

   SNAPf tag

   Expression

   Mammalian

   Available Since

   June 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1(+)-Mito-CaNARi

   Plasmid

   #64851

   Purpose

   Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of mitochondrial outer membrane.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   CaNARi
   

   Tags

   Cerulean, N-terminal 30 amino acids from DAKAP1, …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Orai1

   Plasmid

   #12199

   Depositor

   Anjana Rao

   Article

   Feske et al Nature. 2006 May 11. 441(7090):179-85.

   Insert

   Orai1 (ORAI1 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   July 20, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pTorPE-IP2.0

   Plasmid

   #109217

   Purpose

   Express IP2.0 in E. coli

   Depositor

   Takeshi Ishihara

   Article

   Hara-Kuge et al PLoS One. 2018 Apr 25;13(4):e0194707. do

   Insert

   IP2.0 (Inverse Pericam 2.0)
   

   Tags

   His-tag

   Expression

   Bacterial

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKC158

   Plasmid

   #134242

   Purpose

   CENP-A Halo expression construct

   Depositor

   Iain Cheeseman

   Article

   Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

   Insert

   CENP-A-Halo tagging construct (CENPA Human)
   

   Use

   Human targeting

   Available Since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET21-pelB-LaM-1

    Plasmid

    #172767

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-1, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-1
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p6895 PHAGE-P CMVT N-HA-hE6AP I wt

    Plasmid

    #37601

    Depositor

    Peter Howley

    Article

    Martinez-Noel et al Mol Cell Biol. 2012 May 29.

    Insert

    E6AP I wt (UBE3A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV tet regulatable

    Available Since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLV-EF1a-V5-Smpd4

    Plasmid

    #120237

    Purpose

    Expresses V5-tagged Smpd4 in lentiviral vector

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Insert

    Smpd4 (Smpd4 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Promoter

    EF1a

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHH0103_PIKFYVE_DEP

    Plasmid

    #109789

    Purpose

    Protein expression and purification of PIKFYVE_DEP

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    PIKFYVE_DEP (PIKFYVE Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET21-pelB-LaG-29

    Plasmid

    #172753

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-29; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-29
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSTAP-seq_human-4xUAS

    Plasmid

    #125150

    Purpose

    Vector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in human cells

    Depositor

    Alexander Stark

    Article

    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

    Insert

    4 x upstream activating sequence (UAS)
    

    Use

    Stap-seq screening vector

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1(+)-sapphireAKAR-NLS

    Plasmid

    #118467

    Purpose

    cp-T-sapphire-based single-color PKA biosensor targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    sapphireAKAR-NLS
    

    Tags

    Nuclear localization signal (NLS)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSEM390 - mosTI unc-119 - Peft-3 - NNN - tbb-2

    Plasmid

    #182350

    Purpose

    Ubiquitous expression cloning vector for mosTI(unc-119). Transgene inserted between Peft-3 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSR02

    Plasmid

    #69149

    Purpose

    Read-out loxP mCherry to GFP switch for integration on CxTi10816, Mos chr IV

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Inserts

    mCherry
    eGFP
    

    Use

    Cre/Lox; Mossci

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0

    Promoter

    rps-27

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ZJOM103

    Plasmid

    #133664

    Purpose

    Integration of SEC7-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-SEC7-mTagBFP2 (SEC7 Budding Yeast)
    

    Tags

    mTagBFP2

    Expression

    Yeast

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. BC1441-mouse U6-3xsgRNAs (TS5, TS6, TS7)

    Plasmid

    #164040

    Purpose

    Expression of three sgRNAs (sgTS5, sgTS6, sgTS7) for targeting to CRISPR-Tag_V2

    Depositor

    Baohui Chen

    Article

    Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

    Insert

    sgTS5-sgTS6-sgTS7
    

    Use

    CRISPR

    Promoter

    mouse U6

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge