161,472 results

1. pCDH-Flag-hS1pr1-GFP-Neo

   Plasmid

   #203708

   Purpose

   Overexpression of FLAG-S1PR1-GFP fusion.

   Depositor

   Timothy Hla

   Insert

   Flag-hS1PR1-GFP (S1PR1 Human)
   

   Use

   Lentiviral

   Tags

   Flag and GFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-Gα13*-BERKY3

   Plasmid

   #158218

   Purpose

   BRET biosensor for detection of Gα13-GTP in mammalian expression vector pcDNA3.1(+)

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   Lyn11-Nluc-ER/K linker-3xYFP-PRG(RH)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19/NLS-mChe

   Plasmid

   #183162

   Purpose

   Construct for transient expression of cell nucleus-targeted mCherry in plants.

   Depositor

   Qinlong Zhu, Yao-Guang Liu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Insert

   NLS-mCherry
   

   Use

   Tags

   16 aa nuclear localization signal and 16 aa nucl…

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti6-V5-Topo mCherry

   Plasmid

   #128062

   Purpose

   Lentiviral vector for C terminal mCherry fusions

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBB535

   Plasmid

   #27392

   Depositor

   Bernd Bukau

   Article

   de Marco et al BMC Biotechnol. 2007 . 7():32.

   Insert

   dnaK, dnaJ (dnaJ, dnaK E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-EGFP(HiBiT)

   Plasmid

   #162589

   Purpose

   N-terminally HiBiT-tagged EGFP under CAG promoter

   Depositor

   Masaharu Somiya

   Article

   Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

   Insert

   EGFP
   

   Use

   Tags

   HiBiT

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. p-AAV-sh[control]

   Plasmid

   #75438

   Purpose

   control shRNA vector

   Depositor

   Edward Burton

   Article

   Zharikov et al J Clin Invest. 2015 Jul 1;125(7):2721-35

   Insert

   GFP
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-GFP

   Plasmid

   #106281

   Purpose

   A version of the pHAGE vector with ZsGreen dsRed expressed separated by an IRES. Note that there is little-to-no expression of dsRed.

   Depositor

   Jay Shendure

   Article

   Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

   Insert

   CMV-GFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSRE SSG

   Plasmid

   #127692

   Purpose

   Plasmid expresses the Sindbis Structural Genome (SSG) via the serum response element (SRE) promoter. See Resource Information section for annotated plasmid sequence.

   Depositor

   Bryan Roth, Justin English

   Article

   English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

   Insert

   Sindbis Structural Genome
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 3, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pBB542

    Plasmid

    #27395

    Depositor

    Bernd Bukau

    Article

    de Marco et al BMC Biotechnol. 2007 . 7():32.

    Insert

    dnaK, dnaJ, groESL
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMIR-FLAG-MLL-AF9

    Plasmid

    #71444

    Purpose

    Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-dsRed Express2

    Depositor

    Daisuke Nakada

    Article

    Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

    Insert

    MLL-AF9 (KMT2A Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. QPM-sgR (pTaU3)

    Plasmid

    #140448

    Purpose

    For sgRNA expression in monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

    Insert

    TaU3-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    TaU3

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTP955

    Plasmid

    #104164

    Purpose

    Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (3x Cysteines)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    Anti-rabbit IgG Fc specific nanobody TP897 (3xCysteines)
    

    Use

    Tags

    14xHistidine tag and NEDD8 from Brachypodium dist…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. Nup133_mEGFP(-8a)

    Plasmid

    #163417

    Purpose

    Mammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-8 amino acids)

    Depositor

    Sanford Simon

    Insert

    Nup133 (NUP133 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. phage UbiC tagRFP-T-DDX6

    Plasmid

    #119947

    Purpose

    Lentiviral vector expressing tagRFP-T-DDX6 fusion protein

    Depositor

    Jeffrey Chao

    Article

    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    DDX6 (DDX6 Human)
    

    Use

    Lentiviral

    Tags

    tagRFP-T

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    UbiC

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. TfR-pHuji

    Plasmid

    #61505

    Purpose

    Human transferrin receptor tagged with pH sensitive red fluorescent protein pHuji

    Depositor

    David Perrais

    Article

    Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

    Insert

    Transferrin receptor (TFRC Human)
    

    Use

    Tags

    pHuji

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-GFP1-10-HygR

    Plasmid

    #223675

    Purpose

    To check the split GFP signals in mitochondria (1)

    Depositor

    Juan Melero-Martin

    Article

    Lin et al Nature. 2024 May;629(8012):660-668. doi:

    Insert

    GFP1-10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.GFAP.iGluSnFR3.v857.GPI

    Plasmid

    #178338

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV GFAP iGluSnFR3 v857.GPI
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    GPI Anchor, IgK-chain, and Myc epi tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUAS-V5-TurboID-NES

    Plasmid

    #116904

    Purpose

    Expresses TurboID under Gal4/UAS control. TurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.

    Depositor

    Norbert Perrimon

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID
    

    Use

    Drosophila expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. wt Opa1

    Plasmid

    #204427

    Purpose

    Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 protein.

    Depositor

    Keir Neuman

    Article

    Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

    Insert

    hOpa1 isoform 1 (OPA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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