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We narrowed to 69,601 results for: Cel

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Showing: 7541 - 7560 of 69601 results

  2. pDNR-SpCas9-Gem

    Plasmid
    #80424
    Purpose
    Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining
    Depositor
    Sara Howden, Melissa Little
    Article
    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-
    Insert
    SpCas9-Gem
    Use
    CRISPR
    Tags
    Geminin
    Mutation
    codon optimized for human
    Promoter
    T7
    Available Since
    Aug. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    80424-plasmid-map-sequence-id-151441

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  8. pMT-sox10-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80062
    Purpose
    Sox10 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    Sox10 promoter
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80062-plasmid-map-sequence-id-417618

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  10. EBA175-bio

    Plasmid
    #47743
    Purpose
    Expresses enzymatically monobiotinylated full-length EBA175 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised EBA175
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47743-plasmid-map-sequence-id-68751

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  12. pCAGGS-hPhyBY276H-mCherry-HRasCT

    Plasmid
    #100540
    Purpose
    Expresses Phytochrome B-Y276H (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cells
    Depositor
    Kazuhiro Aoki
    Article
    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p
    Insert
    Phytochrome B (1-908 aa, Y276H mutation) (PHYB Mustard Weed)
    Tags
    mCherry fused with HRas C-terminus for plasma mem…
    Expression
    Mammalian
    Mutation
    Human codon optimized PhyB (1-908 aa), Y276H muta…
    Promoter
    CAG promoter
    Available Since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    100540-plasmid-map-sequence-id-197261

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  13. RH2b-bio

    Plasmid
    #47778
    Purpose
    Expresses enzymatically monobiotinylated partial RH2b ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised RH2b (partial)
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47778-plasmid-map-sequence-id-68797

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  14. pCAGGS-hPhyB-mCherry-HRasCT

    Plasmid
    #100281
    Purpose
    Expresses Phytochrome B (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cells
    Depositor
    Kazuhiro Aoki
    Article
    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p
    Insert
    Phytochrome B (PHYB Mustard Weed)
    Tags
    mCherry fused with HRas C-terminus for plasma mem…
    Expression
    Mammalian
    Mutation
    Human codon optimized PhyB (1-908 aa)
    Promoter
    CAG promoter
    Available Since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    100281-plasmid-map-sequence-id-197245

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  16. pDONR223_HyPer7RgDAAO

    Plasmid
    #217746
    Purpose
    Donor vector for gateway cloning of HyPer7 DAAO, a fusion protein used to measure the uptake of D-amino acids across the plasma membrane.
    Depositor
    Seth Parker
    Article
    Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi
    Insert
    HyPer7 D amino acid oxidase
    Tags
    Nuclear export signal
    Expression
    Mammalian
    Mutation
    Fused DAAO to the C-terminus of HyPer7 using a Gl…
    Available Since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    217746-plasmid-map-sequence-id-442633

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  20. PX-TIR-GFP

    Plasmid
    #52737
    Purpose
    Retroviral vector expresses the lipid binding domain of p40-phox fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable marker
    Depositor
    Jonathan Kagan
    Article
    Bonham et al Cell. 2014 Feb 13;156(4):705-16. doi: 10
    Insert
    Toll/IL-1 Receptor adaptor protein (TIRAP Human)
    Use
    Retroviral
    Tags
    GFP and p40 Phox lipid binding domain
    Expression
    Mammalian
    Mutation
    Deleted amino acids 1-85
    Available Since
    June 4, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 7541 - 7560 of 69601 results