We narrowed to 13,355 results for: yeast expression

1. pCRISPRyl

   Plasmid

   #70007

   Purpose

   CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2015 Dec 29.

   Inserts

   Codon optimized Cas9 from S. pyogenes
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Promoter

   SCR1'-tRNA and UAS1B8-TEF(136)

   Available Since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. TurboID-V5_pRS415

   Plasmid

   #107167

   Purpose

   expresses V5-tagged TurboID in the yeast cytosol

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Expression

   Yeast

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRCC-N

   Plasmid

   #81192

   Purpose

   Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

   Depositor

   Eckhard Boles

   Article

   Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

   Inserts

   Cas9
   empty gRNA cassette
   

   Use

   CRISPR

   Tags

   NTS

   Expression

   Yeast

   Mutation

   Please see depositor comments below. and WT - cod…

   Promoter

   ROX3 and SNP52p

   Available Since

   Sept. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCTCON2 Aga2P-HRP

   Plasmid

   #73152

   Purpose

   Full-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression.

   Depositor

   Alice Ting

   Article

   Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   Aga2P-HRP
   

   Tags

   HA tag and myc tag

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. p415-PtrpC-Cas9-TtrpC-CYC1t

   Plasmid

   #68059

   Purpose

   Express humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.

   Depositor

   Christian Hong

   Article

   Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

   Insert

   humanized Cas9
   

   Use

   CRISPR; N. crassa, fungi

   Expression

   Yeast

   Available Since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIW447-CRISPR XYL2

   Plasmid

   #98906

   Purpose

   K. marxianus CRISPR Plasmid for XYL2 editing

   Depositor

   Ian Wheeldon

   Article

   Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

   Inserts

   Codon optimized Cas9
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40

   Expression

   Yeast

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRS406 ATG9 stopGFP

   Plasmid

   #114669

   Purpose

   Expresses yeast ATG9 under the endogenous promoter with a stop codon introduced before GFP C-terminal tag

   Depositor

   Daniel Klionsky

   Article

   Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

   Insert

   ATG9
   

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Changed the three linker base pairs after the las…

   Promoter

   ATG9

   Available Since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS406-PHO5-GFP-OSBP PH domain

   Plasmid

   #58840

   Purpose

   Expresses GFP-Tagged OSBP PH domain in yeast

   Depositor

   Sean Munro, Tim Levine

   Article

   Levine et al Curr Biol. 1998 Jun 18;8(13):729-39.

   Insert

   Oxysterol-binding Protein PH domain (OSBP Human)
   

   Tags

   GFP

   Expression

   Yeast

   Promoter

   PHO5

   Available Since

   Jan. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pETCON-TNF

   Plasmid

   #67748

   Purpose

   Tumor Necrosis Factor Alpha Yeast Display Plasmid

   Depositor

   Timothy Whitehead

   Article

   Kowalsky et al J Biol Chem. 2015 Oct 30;290(44):26457-7

   Insert

   TNF-Alpha
   

   Expression

   Bacterial and Yeast

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pB194

    Plasmid

    #61154

    Purpose

    Expression of KIN28 under GAL1 promoter in yeast

    Depositor

    Charles Brenner

    Article

    Bieganowski et al J Biol Chem. 2002 Mar 29;277(13):10852-6

    Insert

    TFIIH complex serine/threonine-protein kinase subunit KIN28 (KIN28 Budding Yeast)
    

    Expression

    Yeast

    Promoter

    Gal1

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pRS315-PGK-SEIPIN1

    Plasmid

    #96986

    Purpose

    Express GFP-tagged Arabidopsis SEIPIN1 gene in yeast cells.

    Depositor

    Kent Chapman

    Article

    Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

    Insert

    SEIPIN1 (AT5G16460 Mustard Weed)
    

    Expression

    Yeast

    Promoter

    Phosphoglycerate Kinase (PGK) promoter

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRS315e_pGal-dCas9-SH3

    Plasmid

    #79613

    Purpose

    Expresses dCas9-SH3 in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Tags

    SH3 domain

    Expression

    Yeast

    Mutation

    D10A and H840A for nuclease deficient Cas9

    Promoter

    pGal1

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFA6a-kanMX6-P41nmt1-mEGFP

    Plasmid

    #105140

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Laporte et al Mol Biol Cell. 2012 Aug;23(16):3094-110.

    Insert

    kanMX6-P41nmt1-mEGFP
    

    Tags

    41nmt1-mEGFP

    Expression

    Bacterial and Yeast

    Mutation

    A206K

    Promoter

    41nmt1

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRS416-PHO5-GFP-2xOsh2 PH domain EE mutation

    Plasmid

    #58830

    Purpose

    Expresses GFP-Tagged and mutated Osh2 PH domain in yeast

    Depositor

    Tim Levine

    Article

    Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

    Insert

    2 x Osh2 PH domain (OSH2 Budding Yeast)
    

    Tags

    GFP and Myc

    Expression

    Yeast

    Mutation

    changed Lysine 307 to Glutamate and Arginine 309 …

    Promoter

    PHO5

    Available Since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pFA6a-kanMX6-P3nmt1-mEGFP

    Plasmid

    #105137

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Laporte et al Mol Biol Cell. 2012 Aug;23(16):3094-110.

    Insert

    kanMX6-P3nmt1-mEGFP
    

    Tags

    3nmt1-mEGFP

    Expression

    Bacterial and Yeast

    Mutation

    A206K

    Promoter

    3nmt1

    Available Since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFA6a-kanMX6-P81nmt1-mEGFP

    Plasmid

    #105143

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Laporte et al Mol Biol Cell. 2012 Aug;23(16):3094-110.

    Insert

    kanMX6-P81nmt1-mEGFP
    

    Tags

    81nmt1-mEGFP

    Expression

    Bacterial and Yeast

    Mutation

    A206K

    Promoter

    81nmt1

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMDC84-ScSEIPIN-Ndelta

    Plasmid

    #96985

    Purpose

    Express yeast SEIPIN gene in plants.

    Depositor

    Kent Chapman

    Article

    Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

    Insert

    ScSEIPIN (SEI1 Budding Yeast)
    

    Tags

    6xHis and GFP

    Expression

    Plant

    Mutation

    N-terminal 39 nucleotides were deleted from yeast…

    Promoter

    35S

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFS345

    Plasmid

    #89349

    Purpose

    Beetle Luciferase C-term tagging vector

    Depositor

    Nick Rhind

    Article

    Keifenheim et al Curr Biol. 2017 May 22;27(10):1491-1497.

    Insert

    Beetle luciferase
    

    Use

    C-terminal tagging vector

    Available Since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. PP310

    Plasmid

    #78979

    Purpose

    245R. Promoter: GAP, minimal Promoter: AOX1. Figure 5. Expresses GFP and RFP.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Inserts

    GFP
    RFP
    

    Expression

    Yeast

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. PP362

    Plasmid

    #78981

    Purpose

    255R. Promoter: scTEF1, minimal Promoter: GAP. Figure 5. Expresses GFP and RFP.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Inserts

    GFP
    RFP
    

    Expression

    Yeast

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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