We narrowed to 2,634 results for: BFP

Showing: 1481 - 1520 of 2634 results

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pLKO.1-mTagBFP2-rMPC1 shRNA

Plasmid

#229016

Purpose

Expression of an shRNA construct that knocks down rat MPC1. This plasmid also encodes a blue fluorescent protein tag to verify transfection

Depositor

Ghazaleh Ashrafi

Article

Tiwari et al Sci Adv. 2024 Nov 15;10(46):eadp7423. do

Insert

MPC1 (Mpc1 Rat)

Use

Lentiviral

Tags

mTagBFP2

Expression

Mammalian

Promoter

U6

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMRX-IPU-mTagBFP2-Sec61B

Plasmid

#228136

Purpose

Expresses mTagBFP2-Sec61B in mammalian cells

Depositor

Noboru Mizushima

Article

Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

Insert

Sec61B (SEC61B Human)

Use

Retroviral

Tags

mTagBFP2

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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SOX2-P2A-tagBFP-HDR

Plasmid

#163751

Purpose

HDR knock-in template for tagging the human endogenous SOX2 gene with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

SOX2 (SOX2 Human)

Use

CRISPR and Synthetic Biology

Tags

P2A-tagBFP

Mutation

Does not contain start codon to avoid random inte…

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pME V5-mTagBFP2 (JDW 830)

Plasmid

#224484

Purpose

Gateway compatible middle entry clone containing V5 tagged mTagBFP2 (Cytosolic blue fluorescent reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

V5-mTagBFP2

Use

Gateway Cloning

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pDRF1 yeBFP2-T2A-mCherry

Plasmid

#219851

Purpose

equimolar expression of yeBFP2 and mCherry in budding yeast

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026 Apr 1. doi: 10.1021

Insert

yeBFP2-T2A-mCherry

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1 mTagBFP2 rat DJ-1 shRNA

Plasmid

#222870

Purpose

Expresses mTagBFP2 along with an shRNA against rat DJ-1

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

DJ-1 shRNA (Park7 Rat)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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TagBFP2-C1-SACM1LdeltaTMD-C389S-Fis1tail

Plasmid

#220079

Purpose

SAC1 with a C389S mutation in it with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Blue Fluorophore

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

SACM1l-C389S-Fis1tail (SACM1L Human)

Expression

Mammalian

Mutation

C389SdeltaTMD

Available Since

May 30, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(GATA3(13))-PGKpuro2ABFP-W

Plasmid

#208548

Purpose

Lentiviral vector expressing gRNA targeting human GATA3

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

GATA3(13) (GATA3 Human)

Use

Lentiviral

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(BCL11B-E1(14))-PGKpuro2ABFP-W

Plasmid

#208554

Purpose

Lentiviral vector expressing gRNA targeting human BCL11B-E1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

BCL11B-E1(14) (BCL11B Human)

Use

Lentiviral

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(GATA3(12))-PGKpuro2ABFP-W

Plasmid

#208547

Purpose

Lentiviral vector expressing gRNA targeting human GATA3

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

GATA3(12) (GATA3 Human)

Use

Lentiviral

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(SMARCA2(47))-PGKpuro2ABFP-W

Plasmid

#200510

Purpose

Lentiviral vector expressing gRNA targeting human SMARCA2

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

SMARCA2(47) (SMARCA2 Human)

Use

Lentiviral

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(SMARCA4(15))-PGKpuro2ABFP-W

Plasmid

#200462

Purpose

Lentiviral vector expressing gRNA targeting human SMARCA4

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

SMARCA4(15) (SMARCA4 Human)

Use

Lentiviral

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(SMARCA4(43))-PGKpuro2ABFP-W

Plasmid

#200464

Purpose

Lentiviral vector expressing gRNA targeting human SMARCA4

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

SMARCA4(43) (SMARCA4 Human)

Use

Lentiviral

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(SMARCA2(46))-PGKpuro2ABFP-W

Plasmid

#200465

Purpose

Lentiviral vector expressing gRNA targeting human SMARCA2

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

SMARCA2(46) (SMARCA2 Human)

Use

Lentiviral

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(GFP)-PGKpuro2ABFP-W

Plasmid

#200475

Purpose

Lentiviral vector expressing gRNA targeting GFP

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

GFP

Use

Lentiviral

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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FKBP12-CB2x2-mTagBFP2-HAx2

Plasmid

#207146

Purpose

Tandem calcium-buffer with rapalog dimerizer

Depositor

Antony Galione

Article

Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

Insert

Calbindin-2 (CALB2 Human)

Tags

FKBP12, HA, and mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW yomTagBFP2-T2A-mCherry

Plasmid

#205493

Purpose

Expression of yomTagBFP2-T2A-mCherry in yeast

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026 Apr 1. doi: 10.1021

Insert

yomTagBFP2-T2A-mCherry

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-mTagBFP2-WPRE

Plasmid

#193332

Purpose

Expresses mTagBFP2 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

mTagBFP2

Expression

Mammalian

Mutation

A XhoI site in the coding sequence is silenced

Promoter

TRE

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-FKBP-mTagBFP2-MCS

Plasmid

#178864

Purpose

Mammalian expression of a protein of interest fused to the C-terminus of FKBP-mTagBFP2

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

FKBP-mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-pCaMKII-EBFP-tTAn

Plasmid

#172124

Purpose

Expression of EBFP and tTA-InteinN in CaMKII positive cells

Depositor

Chun Xu

Article

Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

Insert

pCaMKII, EBFP, tTAn-InteinN

Use

AAV

Promoter

EF1a

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pHR Src deltaSH2/3-TagBFP

Plasmid

#171033

Purpose

TagBFP-tagged Src variant lacking SH2 and SH3 domains.

Depositor

Jared Toettcher

Article

Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

Insert

Src

Use

Lentiviral

Expression

Mammalian

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAC-U63-tgRNA-nlsBFP

Plasmid

#169029

Purpose

gRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a Ubi-mTagBFP-NLS marker.

Depositor

Chun Han

Article

Allen et al PLoS Biol. 2021 Jan 14;19(1):e3001061. d

Type

Empty backbone

Use

CRISPR

Expression

Insect

Promoter

U6:3

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pBlastR-12xLexAOp-EBFP2-MiniWhite

Plasmid

#165918

Purpose

Blasticidin resistant 12xLexAOp EBFP2 Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable LexAOp Response Vector

Use

Synthetic Biology

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(gKAT7-5)-PGKpuro2ABFP-W

Plasmid

#159287

Purpose

Lentiviral vector expressing gRNA targeting KAT7 (gRNA ID. 5)

Depositor

Kosuke Yusa

Article

Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

Insert

guide RNA targeting KAT7 (ID. 5) (KAT7 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

human U6 promoter

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pTagBFP2-C1-AKAP1N31-FKBP-PI-PLCN187

Plasmid

#139309

Purpose

N-terminal half of split PI-PLC targeted to mitochondria

Depositor

Gerry Hammond

Article

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Mus musculus Akap1(1-31) M16L: PTRSANS[GA]4ILSRM:TagBFP2: FKBP1A(3-108): [GGSA]4GG:L. monocytogenes PI-PLC(1-187)

Expression

Mammalian

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Pou5f1-g2)-PGKpuroBFP-W

Plasmid

#105036

Purpose

Lentiviral gRNA plasmid targeting mouse Pou5f1 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Pou5f1 (Pou5f1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Stat3)-PGKpuroBFP-W

Plasmid

#105027

Purpose

Lentiviral gRNA plasmid targeting mouse Stat3 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Stat3 (Stat3 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Ptpn23-g1)-PGKpuroBFP-W

Plasmid

#105034

Purpose

Lentiviral gRNA plasmid targeting mouse Ptpn23 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Ptpn23 (Ptpn23 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Vps39-g1)-PGKpuroBFP-W

Plasmid

#105019

Purpose

Lentiviral gRNA plasmid targeting mouse Vps39 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Vps39 (Vps39 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Char-BFP2-TSERex

Plasmid

#124792

Purpose

Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

Depositor

Yulong Li

Article

Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

Insert

Char-BFP2

Expression

Mammalian

Promoter

CAG

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAS1-rab-3P::his-24::tagBFP

Plasmid

#124343

Purpose

Nuclear Localized Pan-Neuronal tagBFP

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

rab-3, tagBFP (rab-3 Nematode)

Expression

Worm

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR CMVie-Lifeact-mTagBFP2 L1-R5

Plasmid

#84388

Purpose

Multisite Gateway entry vector for expression of mTagBFP2-tagged Lifeact in cells including neurons

Depositor

Ghassan Mouneimne

Insert

Lifeact

Use

Gateway Cloning

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV immediate early

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pSLPB2B-FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

Plasmid

#187951

Purpose

FKBP12 (F36V mutant) degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Insert

FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP

Use

CRISPR and Synthetic Biology

Tags

GGS linker

Expression

Mammalian

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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p2710-pHAGE2-Ef1aL-wtYAP-UBC-tagBFP-loxP

Plasmid

#216479

Purpose

lentiviral dual expression of wtYAP (YAP1) and tagBFP with loxP site for Cre excision

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Inserts

wtYAP (YAP1) (YAP1 Human)
tagBFP

Use

Cre/Lox and Lentiviral

Promoter

EF1aL and UBC

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-RNF169

Plasmid

#119004

Purpose

Mammalian expression of human RNF169 and BFP

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

RNF169 (RNF169 Human)

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCS4077

Plasmid

#131738

Purpose

pLenti_CMV-mCherry-BghA_EF1alpha-BFP

Depositor

Christina Smolke

Article

Xiang et al Nat Commun. 2019 Sep 23;10(1):4327. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pIM573

Plasmid

#74637

Purpose

alias pFA6a-ymTag-BFP-kanMX6 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and G418 selection marker

Depositor

Jiří Hašek

Article

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Insert

ymTagBFP

Expression

Yeast

Promoter

none

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS-Rox-nlsKate5V-stop-Rox-myr-Flag-BFP-NEO (JDW 473)

Plasmid

#242587

Purpose

A CAGGS driven, Dre recombinase dependent switch reporter (nls-mKate2 to MbBFP following Dre recombination).

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

nlsKateV5, MbBPF-FLAG, FRTNeoFRT

Expression

Mammalian

Promoter

CAGGS

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pTol2-Ubi-lsl-EGFP-nls-mCherry:fli1aep-CreI-zf1-BFP (JDW 1236)

Plasmid

#229816

Purpose

A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with fli1-TagBFP-zCre in the opposite direction.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

loxp-EGFP-stop-lox

Use

Tol2 based expression vector

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pTol2-Hsp70-H2B-mCerulean-lsl-mScarlet-fli1-zCre-I-BFP (JDW 1233)

Plasmid

#229841

Purpose

A Tol2 based expression vector with the Hsp70 promoter driving a cre dependent switch reporter. Contains an endothelial driven cre

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

loxp-H2B-mCerulean-2xStop-loxP

Use

Cre/Lox; Tol2 based expression vector

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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