We narrowed to 4,845 results for: AAT

1. pCMV-dLight1.2

   Plasmid

   #111054

   Purpose

   Expresses dLight1.2 in mammalian cells

   Depositor

   Lin Tian

   Article

   Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

   Insert

   dLight1.2
   

   Use

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. BRCA2 A12.2 gRNA

   Plasmid

   #90551

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human BRCA2

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   BRCA2 (Guide Designation A12.2)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. PMXS-SLC1A3

   Plasmid

   #72873

   Purpose

   The retroviral SLC1A3 vector was generated by cloning an human SLC1A3 PCR fragment (https://www.addgene.org/32813/)into the pMXS-ires-blast vector by Gibson Assembly

   Depositor

   David Sabatini

   Article

   Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

   Insert

   SLC1A3 (SLC1A3 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pRARE-MBP-DEST

   Plasmid

   #84650

   Purpose

   Gateway cloning vector expresses MBP-tagged proteins under the control of the IPTG– inducible ptac promoter and also expresses several tRNAs that are rare in E. coli.

   Depositor

   Neil Olszewski

   Article

   Steiner et al Plant Cell. 2012 Jan;24(1):96-108. doi:

   Type

   Empty backbone

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. shTBX5 # 1

   Plasmid

   #42549

   Depositor

   William Hahn

   Article

   Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

   Insert

   shTBX5 (TBX5 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pORANGE Bsn-GFP KI

   Plasmid

   #139665

   Purpose

   Endogenous tagging of Bassoon: C-terminal (amino acid position: F3938)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28b-HRASLS3-1-132

   Plasmid

   #100725

   Purpose

   Expression of N-terminal region of human HRASLS3 in E.coli

   Depositor

   Byung Il Lee

   Article

   Han et al Protein Expr Purif. 2010 May;71(1):103-7

   Insert

   HRASLS3 (PLAAT3 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promotor

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5FRT/TO-UBE2QL1-Strep/HA

   Plasmid

   #124666

   Purpose

   Expresses UBE2QL1 with C-terminal Strep-HA tag in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

   Insert

   UBE2QL1 (UBE2QL1 Human)
   

   Use

   Tags

   2xStrep/HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5FRT/TO-UBE2QL1-CS-Strep/HA

   Plasmid

   #129513

   Purpose

   Expresses UBE2QL1 C88S (catalytically inactive mutant) with C-terminal Strep-HA tag in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

   Insert

   UBE2QL1 C88S (UBE2QL1 Human)
   

   Use

   Tags

   2xStrep/HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFEndoNA2

    Plasmid

    #174667

    Purpose

    Expresses GFP-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.

    Depositor

    Jukka Finne

    Article

    Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149

    Insert

    GFP-endoNA2 fusion protein
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-nLight3.1

    Plasmid

    #111061

    Purpose

    Expresses nLight3.1 in mammalian cells

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Insert

    nLight3.1
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTC-ApoE-Tet-YAP

    Plasmid

    #85580

    Purpose

    Sleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent YAP expression (ApoE.HCR.hAAT promoter) in mouse liver

    Depositor

    Ursula Ehmer

    Article

    Hubner et al J Gene Med. 2016 Dec 23. doi: 10.1002/jg

    Inserts

    CreER
    YAP
    

    Use

    Cre/Lox

    Tags

    Flag (2x)

    Expression

    Mammalian

    Mutation

    Promoter

    ApoE.HCR.hAAT and TRE

    Available since

    Jan. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmIL-6 mut C/EBP

    Plasmid

    #61291

    Purpose

    drives luciferase from mouse IL-6 promoter with mutant C/EBP (NF-IL-6) site

    Depositor

    Gail Bishop

    Article

    Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.

    Insert

    IL-6 promoter (Il6 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Mutated C/EBP binding site from ACATTGTGCAATCT to…

    Promoter

    IL-6 promoter with mutant C/EBP binding site

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pSico-shRaptor

    Plasmid

    #81086

    Purpose

    conditional expression of an shRNA targeting murine Raptor

    Depositor

    Angelique Bordey

    Article

    Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

    Insert

    Raptor shRNA (Rptor Mouse)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-PKI nls

    Plasmid

    #118301

    Purpose

    Expression of PKIa

    Depositor

    Philip Stork

    Article

    Billiard et al J Biol Chem. 2001 Aug 17;276(33):31238-4

    Insert

    PKI nls (aa 2-36)
    

    Use

    Tags

    Flag and GFP

    Expression

    Mammalian

    Mutation

    inserting 2xnls

    Promoter

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSMP-Bmi1_3

    Plasmid

    #36354

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    Bmi1 (BMI1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pMXs_FLAG-SFXN1

    Plasmid

    #110634

    Purpose

    Retroviral expression of Flag-SFXN1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Science. 2018 Nov 16;362(6416). pii: 362

    Insert

    SFXN1 (SFXN1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon-optimized cDNA (no amino acid mutations)

    Promoter

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. LentiGuide-Neo-CTRLg2

    Plasmid

    #139451

    Purpose

    Lentiviral vector with non-targeting gRNA and neomycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    Non-targeting sgRNA inserted; resistance gene: neoR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-dLight1.3a

    Plasmid

    #111055

    Purpose

    Expresses dLight1.3a in mammalian cells

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Insert

    dLight1.3a
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCfB3051(gRNA X-3 XI-2 XII-2)

    Plasmid

    #73293

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-3, XI-2, and XII-2

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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