We narrowed to 923 results for: Mtor

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pRK5-FLAG-FLCN

Plasmid

#72290

Purpose

overexpression

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FLCN (FLCN Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pMXs-IP- SCAT3.2

Plasmid

#172497

Purpose

SCAT3.2 is fluorescent reporter (FRET probe) for cell survival/apoptosis with Caspase-3 sensitive sequence which can be introduced to mammalian cells by retrovirus vector.

Depositor

David Scadden

Article

Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

Insert

Amcyan

Use

Retroviral

Tags

mVenus

Expression

Mammalian

Promoter

MLV LTR

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLV_3XHA-ATP6V1B2

Plasmid

#228864

Purpose

Stable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA

Depositor

Wilhelm Palm

Article

Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

Insert

ATP6V1B2 (ATP6V1B2 Human)

Use

Lentiviral

Tags

3xHA

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-Eef2TOP-RLCP-HCVIRES-FL

Plasmid

#233628

Purpose

Expression of bicistronic reporter with TOP motif

Depositor

Shintaro Iwasaki

Article

Shichino et al Nat Struct Mol Biol. 2024 Oct;31(10):155

Insert

Promoter and 5'UTR of Eef2 gene and HCV IRES (Eef2 Mouse)

Use

Luciferase

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pIS1-Vim/Actb5UTR-renilla

Plasmid

#38238

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Vim promoter with Actb 5UTR/Renilla (Vim, Actb Mouse)

Use

Luciferase

Expression

Mammalian

Mutation

Actb promoter (1000 nt upstream) and 5' UTR …

Promoter

Endogenous Vim

Available Since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only
pCAGGS-4EBP1 F113A

Plasmid

#81122

Purpose

expresses a constitutively active mutant of 4E-BP1 under the CAG promoter

Depositor

Angelique Bordey

Article

Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

Insert

4EBP1 F113A (Eif4ebp1 Mouse)

Expression

Mammalian

Mutation

F113A mutation

Promoter

CAG

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-hAtg13

Plasmid

#22875

Depositor

Noboru Mizushima

Article

Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

Insert

autophagy-related genes 13 (ATG13 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only
pPB-EF1A-Puro-hTBK1-E696K-EGFP

Plasmid

#221554

Purpose

Expresses GFP-tagged human TBK1 with E696K mutation in mammalian cells. Compatible with piggybac transposase for genome integration.

Depositor

Shawn Ferguson

Article

Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

Insert

TBK1 (TBK1 Human)

Use

Piggybac

Tags

GFP

Expression

Mammalian

Mutation

Changed glutamate 696 to lysine

Promoter

EF1a

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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3XFLAG_GAPDH in pCW57_tTA_Blast

Plasmid

#154211

Purpose

Dox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of GAPDH

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

GAPDH (GAPDH Human)

Use

Lentiviral

Tags

3X FLAG

Expression

Mammalian

Promoter

TRE repeats (dox off)

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLJM1 TMEM192-Flag

Plasmid

#112742

Purpose

express pLJM1 TMEM192-Flag

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

TMEM192-Flag (TMEM192 Human)

Use

Lentiviral

Tags

Flag

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-NPC1(D786N)-FLAG

Plasmid

#164975

Purpose

Lentiviral vector for NPC1 expression in mammalian cells

Depositor

Roberto Zoncu

Article

Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

Insert

NPC1 (NPC1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D786N, insertion L aa15 (please see depositor co…

Promoter

EF1a

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLJM60-FLAG-SLC38A9.1

Plasmid

#71858

Purpose

lentiviral stable expression

Depositor

David Sabatini

Article

Wang et al Science. 2015 Jan 9;347(6218):188-94. do

Insert

SLC38A9.1 (SLC38A9 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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C terminal Flag Mios pRK5

Plasmid

#46326

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Mios (MIOS Human)

Tags

Flag

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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myc-Atg14

Plasmid

#86747

Purpose

N-terminal myc-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

Insert

human Atg14 (ATG14 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pMXs-IP-EGFP-hAtg13

Plasmid

#38191

Depositor

Noboru Mizushima

Article

Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

Insert

autophagy-related genes 13 (ATG13 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
pSpCas9(BB)-2A-Puro-C9orf72

Plasmid

#83439

Purpose

Expresses Cas9 and a gRNA targeting C9orf72

Depositor

Shawn Ferguson

Article

Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

Insert

gRNA targeting human C9orf72 (C9orf72 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV10-hAtg13

Plasmid

#22872

Depositor

Noboru Mizushima

Article

Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

Insert

autophagy-related genes 13 (ATG13 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 25, 2010

Availability

Academic Institutions and Nonprofits only
pLJM1-TMEM192-FLAG

Plasmid

#164977

Purpose

Lentiviral vector for TMEM192 expression in mammalian cells

Depositor

Roberto Zoncu

Article

Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

Insert

TMEM192 (TMEM192 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX-Frb:Akt2-IRES-Hygromycin

Plasmid

#120713

Purpose

Expresses Frb:Akt2 fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

AKT2 (AKT2 Human)

Use

Lentiviral

Tags

FRB(T2098L)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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FLCN-eGFP

Plasmid

#72289

Purpose

Expresses human FLCN tagged with eGFP at C terminus

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FLCN (FLCN Human)

Tags

eGFP

Expression

Mammalian

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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