We narrowed to 2,482 results for: PRK

Showing: 741 - 760 of 2482 results

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pRK5-FLAG-Sestrin2-E451A

Plasmid

#73673

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

E451A changed Glutamate 451 to Alanine

Promoter

CMV

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
pRK5-myc-Miro1 S432N

Plasmid

#47893

Purpose

Expresses myc tagged Miro1 S432N dominant negative mutant

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro1 S432N (RHOT1 Human)

Tags

myc

Expression

Mammalian

Mutation

S432N, dominant negative mutant

Promoter

CMV

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-HA-Ubiquitin-K27R-K29R

Plasmid

#121156

Purpose

Mammalian expression of HA tagged ubiquitin K27R and K29R

Depositor

Josef Kittler

Article

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Ubiquitin C (UBC Human)

Expression

Mammalian

Mutation

K27 and K29 mutated to Rs

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pRK DPC4 deltaC Flag

Plasmid

#12628

Depositor

Rik Derynck

Article

Zhang et al Nature. 1996 Sep 12. 383(6596):168-72.

Insert

DPC4 delta C (SMAD4 Human)

Tags

flag

Expression

Mammalian

Mutation

C-terminal truncated DPC4 (aa 1-511)

Available Since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
pRK5-myc-PICK1-rescue

Plasmid

#72573

Purpose

Expresses myc-PICK1 shRNA resistant cDNA

Depositor

Victor Anggono, Richard Huganir

Article

Anggono et al J Neurosci. 2011 Feb 9;31(6):2188-96. do

Insert

PICK1 (Pick1 Rat)

Tags

Myc

Expression

Mammalian

Mutation

5 silent mutations around shRNA recognition seque…

Promoter

CMV

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-RAX-MUT

Plasmid

#194573

Purpose

Mammalian Expression of mEGFP-RAX Mutant (S222Rfs*63) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

RAX (RAX Human)

Tags

mEGFP

Expression

Mammalian

Mutation

S222Rfs*63

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-MYOD1-MUT

Plasmid

#194571

Purpose

Mammalian Expression of mEGFP-MYOD1 Mutant (R188Pfs*90) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

MYOD1 (MYOD1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

R188Pfs*90

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK-ATF4 111-351

Plasmid

#26119

Depositor

Yihong Ye

Article

Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

Insert

ATF4 (ATF4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

aa's 1-110 have been deleted

Available Since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only
pRK5 Flag Grb10 5A short

Plasmid

#37484

Depositor

David Sabatini

Article

Hsu et al Science. 2011 Jun 10;332(6035):1317-22.

Insert

Grb10 5A (GRB10 Human)

Tags

Flag

Expression

Mammalian

Mutation

isoform C (missing N-term 58aa), 536aa, S150A T15…

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only
pRK5 Fyn SH2:R176E

Plasmid

#16035

Depositor

Filippo Giancotti

Article

Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

Insert

c-Fyn (FYN Human)

Expression

Mammalian

Mutation

SH2 point mutant: R176E

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
prk5-TMEM192-3xHA PM mutant

Plasmid

#149506

Purpose

Transient expression of human TMEM192. Dileucine motifs LL23-24 and LL28-29 have been mutated to AA.

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Insert

TMEM192 (TMEM192 Human)

Use

Transient expression

Tags

3XHA

Expression

Mammalian

Mutation

p.LL23-24AA, p.LL28-29AA

Promoter

CMV

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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USP19 (C506A)-FLAG/pRK5

Plasmid

#36307

Depositor

Xiaolu Yang

Article

Mei et al J Biol Chem. 2011 Oct 14;286(41):35380-7

Insert

ubiquitin specific peptidase 19 (USP19 Human)

Tags

FLAG

Expression

Mammalian

Mutation

C506A

Promoter

CMV

Available Since

May 11, 2012

Availability

Academic Institutions and Nonprofits only
pRK7-HA-S6K1-F5A-E389

Plasmid

#8988

Depositor

John Blenis

Article

Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

Insert

S6K1 (Rps6kb1 Rat)

Tags

HA

Expression

Mammalian

Mutation

F5A, T389E

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pRK-ATF4 248-351

Plasmid

#26120

Depositor

Yihong Ye

Article

Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

Insert

ATF4 (ATF4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

ATF4 aa's 1-247 have been deleted

Available Since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only
pRK5 Flag Grb10 9A long

Plasmid

#37485

Depositor

David Sabatini

Article

Hsu et al Science. 2011 Jun 10;332(6035):1317-22.

Insert

Grb10 9A (GRB10 Human)

Tags

Flag

Expression

Mammalian

Mutation

isoform A, 594aa, S104A S150A T155A S158A S426A S…

Available Since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only
pRK5-PLBD2-6xHis S392A

Plasmid

#213607

Purpose

Expresses PLBD2-6xHis S392A in mammalian cells

Depositor

Monther Abu-Remaileh

Article

Nyame et al Mol Cell. 2024 Apr 4;84(7):1354-1364.e9.

Insert

phospholipase B domain containing 2 (PLBD2 Human)

Tags

6xHis

Expression

Mammalian

Mutation

Changed serine 392 to alanine

Promoter

CMV

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5-rat PKA-RIIbeta

Plasmid

#45522

Depositor

Haining Zhong

Article

Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

Insert

PKA regulatory subunit RII beta

Expression

Mammalian

Available Since

June 12, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Miro2 R425V

Plasmid

#47898

Purpose

Expresses myc tagged Miro2 R425V constitutively active mutant

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro2 R425V (RHOT2 Human)

Tags

myc

Expression

Mammalian

Mutation

R425V, constitutively active mutant

Promoter

CMV

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-myc-Miro2 S430N

Plasmid

#47899

Purpose

Expresses myc tagged Miro2 S430N dominant negative mutant

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro2 S430N (RHOT2 Human)

Tags

myc

Expression

Mammalian

Mutation

S430N, dominant negative mutant

Promoter

CMV

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-H1-shRNA-CMV-HA-PACSIN1

Plasmid

#72577

Purpose

Bicistronic construct expressing PACSIN1 shRNA and HA-PACSIN1-rescue construct

Depositor

Richard Huganir

Article

Anggono et al Proc Natl Acad Sci U S A. 2013 Aug 20;11

Inserts

PACSIN1 (Pacsin1 Mouse)
H1-PACSIN1 shRNA cassette

Use

RNAi

Tags

HA

Expression

Mammalian

Mutation

5 silent mutations around shRNA recognition seque…

Promoter

CMV and H1

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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