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pJL017

Plasmid

#53532

Purpose

This plasmid encodes both the etpB and etpA two partner secretion genes on an arabinose-inducible plasmid with myc and 6His tags in-frame with the etpA gene

Depositor

James Fleckenstein

Article

Fleckenstein et al Nat Protoc. 2009;4(7):1083-92. doi: 10.1

Insert

etpBA

Tags

6His and myc

Expression

Bacterial

Promoter

pBAD

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pUB-cHyPer2

Plasmid

#84728

Purpose

Expresses HyPer2 in plant cells-biosensor for Hydrogen peroxide

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

HyPer2

Expression

Plant

Promoter

ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pmRuby2-CrowdingLinker-Clover

Plasmid

#171058

Purpose

Expresses tandem mClover2-mRuby2 FRET pair with a crowdedness-sensitve linker domain to sense cytosolic crowdedness.

Depositor

Ke Xu

Article

Chen et al Light Sci Appl. 2021 May 8;10(1):97. doi

Insert

Crowdedness-sensitive linker domain

Tags

mClover2 and mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pGem-nHyPer2:sHyper2

Plasmid

#84737

Purpose

Simultaneous expresion of nuclear and stromal HyPer2 in plant cells

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

HyPer2 targeted to nucleus

Tags

NLS-SV40

Expression

Plant

Promoter

pFMV/p35S

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pT3TS-nCas9n-A71

Plasmid

#125644

Purpose

micro-injection of poly-adenylated nCas9n RNA

Depositor

Jason Berman

Article

Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

Insert

nCas9n

Use

Rna in vitro transcription vector

Promoter

T3

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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FCK-Arch-CFP

Plasmid

#22221

Purpose

Lentiviral expression of Arch-CFP for optogenetic inhibition

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

Arch

Use

Lentiviral

Tags

CFP

Expression

Mammalian

Available Since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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Lyn-tdnano-HA

Plasmid

#136512

Purpose

Mammalian expression of a tandem dimer of WT SspB (nano), targeted to membrane by Lyn myristoylation sequence, with triple-HA epitope tag

Depositor

Bianxiao Cui

Article

Hope et al J Mol Biol. 2020 Jun 12;432(13):3739-374

Insert

Lyn nano nano 3HA

Expression

Mammalian

Promoter

CMV

Available Since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-C. thermophilum LIC

Plasmid

#74780

Purpose

encodes GST-tagged dynein light intermediate chain from Chaetomium thermophilum

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

dynein light intermediate chain (Chaetomium thermophilum)

Tags

GST

Expression

Bacterial

Promoter

T7

Available Since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

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pgRNA-bacteria_speG_M15

Plasmid

#220488

Purpose

inducible CRISPRi

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

gspeG

Use

CRISPR and RNAi

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pgRNA-bacteria_speG_E23

Plasmid

#220489

Purpose

inducible CRISPRi

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

gspeG

Use

CRISPR and RNAi

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcHis_VectorA_short_speG_genomic

Plasmid

#220473

Purpose

heterologous expression under endogenous speG E. coli promoter

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

speG

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcHis_VectorA_short_speGpromoter_PA4114

Plasmid

#220474

Purpose

heterologous expression of PA4114 under endogenous speG E. coli promoter

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA4114

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcHis_VectorA_short_speGpromoter_PA1472

Plasmid

#220475

Purpose

heterologous expression of PA1472 under endogenous speG E. coli promoter

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA1472

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcHis_VectorA_short_speGpromoter_PA1377

Plasmid

#220476

Purpose

heterologous expression of PA1377 under endogenous speG E. coli promoter

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA1377

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pITESceIcmd

Plasmid

#218288

Purpose

Introduction of a cyanamide-inducible I-SceI expression cassette, use in combination with pITEdv1

Depositor

Claudia Vickers

Article

McDonnell et al bioRxiv 2024.03.26.586389

Insert

HO(-253, -1)-tSynth3

Expression

Yeast

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNAintron-LASV-GPC-c3xFLAG

Plasmid

#215689

Purpose

Produces the Lassa virus glycoprotein with a c-terminal 3xFLAG tag

Depositor

Melinda Brindley

Article

Acciani et al J Virol. 2017 Aug 24;91(18):e00574-17. d

Insert

LASV GP-FLAG

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNCS-Clover4-LUMABS-HA

Plasmid

#183486

Purpose

To express the Clover4-LUMABS-HA sensor for Avian Influenza Virus antibody detection.

Depositor

Jun Chu, Zongwen Jin

Article

Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

Insert

Clover4-LUMABS-HA

Expression

Bacterial

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pNCS-Clover4-LUMABS-DEN1

Plasmid

#183487

Purpose

To express the Clover4-LUMABS-DEN1 sensor for Dengue Virus antibody dection.

Depositor

Jun Chu, Zongwen Jin

Article

Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

Insert

Clover4-LUMABS-DEN1

Expression

Bacterial

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pQlinkH-YbATP-grasp

Plasmid

#129639

Purpose

Expression plasmid of ATP-grasp from Y. bercovieri

Depositor

Matthias Bochtler

Article

Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

Insert

YbATP-grasp

Tags

His6-tag

Expression

Bacterial

Promoter

pQE

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-Chaetomium thermophilum LIC

Plasmid

#74782

Purpose

encodes dynein light intermediate chain (LIC) from Chaetomium thermophilum; no tag

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

Chaetomium thermophilum dynein light intermediate chain

Expression

Bacterial

Promoter

T7

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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