We narrowed to 5,504 results for: pcas

1. pCA-G-intron-T

   Plasmid

   #36885

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   GFP
   tdTomato-3Myc
   beta-globin intron
   

   Use

   Tags

   3 Myc tags

   Expression

   Mammalian

   Mutation

   deleted nucleotides after nucleotide 274, inserti…

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer)

   Available since

   Aug. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGs-Hoxc8

   Plasmid

   #45555

   Depositor

   Jeremy Dasen

   Article

   Lacombe et al PLoS Genet. 2013 Jan;9(1):e1003184. doi:

   Insert

   Hoxc8 (HOXC8 Chicken)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   CMV / b-actin

   Available since

   Aug. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pCAG-GW-V5-Hygro

   Plasmid

   #68265

   Purpose

   Constitutive selectable expression of C-term V5 tagged proteins

   Depositor

   Mitchell Guttman

   Article

   McHugh et al Nature. 2015 May 14;521(7551):232-6. doi

   Type

   Empty backbone

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pCAG-DN-RFX

   Plasmid

   #52296

   Purpose

   Dominant-negatively inhibits RFX (regulatory factor X) transcription factor. Made of mouse RFX1 DNA binding domain (400-527 aa) and C-terminal myc tag.

   Depositor

   Christopher A Walsh

   Article

   Bae et al Science. 2014 Feb 14;343(6172):764-8. do

   Insert

   Dominant negative RFX
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS_2paCRY2(D387A)

   Plasmid

   #129652

   Purpose

   2P-activatable CRY2 with the FAD-disrupting mutation

   Depositor

   Michiyuki Matsuda

   Article

   Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

   Insert

   2paCRY2(D387A)
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCaST-dVP16AD

   Plasmid

   #15306

   Depositor

   Benjamin White

   Article

   Luan et al Neuron. 2006 Nov 9. 52(3):425-36.

   Insert

   dVP16AD-ZIP+
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 6, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS EFF-1::V5

   Plasmid

   #133584

   Purpose

   Expresses EFF-1 A full length protein in mammalian cells

   Depositor

   Benjamin Podbilewicz

   Article

   Avinoam et al Science. 2011 Apr 29;332(6029):589-92. d

   Insert

   EFF-1::V5
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-B2UP49_GFP

   Plasmid

   #103071

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage, also expresses EGFP

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   B2UP49(Cas9 coding gene from Akkermansia muciniphila (strain ATCC BAA-835))
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Promoter

   CMV

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAFNF-Rac1

   Plasmid

   #163607

   Purpose

   Expresses Rac1 Flp-dependently in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Rac1 (Rac1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAD108_Arg273His_pCMV6Entry-hIRG1

    Plasmid

    #124878

    Purpose

    Expresses hCAD Arg273His mutant in mammalian cells

    Depositor

    Konrad Buessow

    Article

    Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

    Insert

    cis-aconitate decarboxylase (ACOD1 Human)
    

    Use

    Tags

    myc and FLAG tags

    Expression

    Mammalian

    Mutation

    mutated Arg273 to His

    Promoter

    CMV

    Available since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Topo_SpCas9.tracr_LbCas12a.DR

    Plasmid

    #155049

    Purpose

    TOPO vector for the cloning of _the SpCas9 tracrRNA - LbCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    SpCas9 tracrRNA and LbCas12a Direct Repeat (DR)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS-S-P1-FLAG

    Plasmid

    #177095

    Purpose

    P1 variant S-protein with c-terminal FLAG tag

    Depositor

    Daniel Conway

    Insert

    S-protein P1 (S SARS-CoV-2)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pCAG-hHDAC4

    Plasmid

    #21096

    Depositor

    Connie Cepko

    Article

    Chen et al Science. 2009 Jan 9. 323(5911):256-9.

    Insert

    Histone Deacetylase 4 (HDAC4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    shRNA target sequence was mutated (mutated nucleo…

    Promoter

    CAG

    Available since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

14. B-SpCas9-HF1

    Plasmid

    #126762

    Purpose

    Expression plasmid for human codon-opt. increased fidelity Blackjack-SpCas9-HF1 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than SpCas9HF1.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    B-SpCas9-HF1
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A; R661A; Q695A; Q926A; amino acids 1005-1013…

    Promoter

    CBh

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-ERas-IH

    Plasmid

    #15688

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Nature. 2003 May 29. 423(6939):541-5.

    Insert

    ERas (Eras Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pCAG-nirButterfly

    Plasmid

    #136590

    Purpose

    Expression of a genetically-encoded voltage indicator nirButterfly

    Depositor

    Vladislav Verkhusha

    Article

    Monakhov et al ACS Chem Neurosci. 2020 Nov 4;11(21):352

    Insert

    nirButterfly
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCA1.332 pDUOTK1-L1

    Plasmid

    #162351

    Purpose

    Level 0 terminator acceptor containing dual fluorophores for testing transcription terminator efficiency

    Depositor

    Alistair McCormick

    Article

    Gale et al Front Microbiol. 2021 Jan 15;11:624011.

    Inserts

    eYFP
    LacZ alpha
    mTagBFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-p21_T145A

    Plasmid

    #40203

    Depositor

    Philipp Mergenthaler

    Article

    Mergenthaler et al J Cereb Blood Flow Metab. 2013 Mar;33(3)

    Insert

    p21 (CDKN1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T145A

    Promoter

    CAG

    Available since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. KA2706_eSpCas9-PGKPacdtkCh

    Plasmid

    #124204

    Purpose

    Expression of eSpCas9(1.1) and sgRNA

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAGGS-Hoxc4

    Plasmid

    #45550

    Depositor

    Jeremy Dasen

    Article

    Lacombe et al PLoS Genet. 2013 Jan;9(1):e1003184. doi:

    Insert

    Hoxc4 (Hoxc4 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    CMV / b-actin

    Available since

    Aug. 5, 2013

    Availability

    Academic Institutions and Nonprofits only