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Plasmid#207340PurposeMammalian expression of HA-Δ71 PPM1HDepositorInsertPPM1H (PPM1H Human)
UseTagsHAExpressionMammalianMutationΔ71PromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOLY_new
Plasmid#179276PurposeExpresses CuCBP, CcCDP and BaSP tricistronically in E. coli BL21(DE3)DepositorInsertscellodextrin phosphorylase
cellobiose phosphorylase
sucrose phosphorylase (C8077_RS00435 Bifidobacterium alodescentis)
UseSynthetic BiologyTags6xHIS and Strep-Tag IIExpressionBacterialMutationPromoterT7 lacOAvailable sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGART-GFP
Plasmid#99106PurposeExpresses human GART-GFP fusion in mammalian cellsDepositorInsertGART (GART Human)
UseTagsGFPExpressionMammalianMutationA65T, L68V, and A72S mutations in GFP gene; kanR/…PromoterCMVAvailable sinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
MBP-FUS_FL_12E
Plasmid#98652PurposeExpresses MBP-tagged full length phosphomimetic FUSDepositorInsertFUS (FUS Human)
UseTags6xHis-MBPExpressionBacterialMutation12 S/T to EPromoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
MBP-FUS_FL_6E
Plasmid#98655PurposeExpresses MBP-tagged full length phosphomimetic FUSDepositorInsertFUS (FUS Human)
UseTags6xHis-MBPExpressionBacterialMutation6 S/T to EPromoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-2xPH-TAPP1 R211L mutant
Plasmid#161991PurposeExpresses two mutated tandem repeats of Tapp1 PH domain (phosphoinositide binding-deficient mutant of TAPP1, R211L, can't bind PI(3,4)P2). Fused to eGFPDepositorInsertPH-TAPP1 (PLEKHA1 Human)
UseTagsExpressionMammalianMutationR211LPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag-M4-AR-S81A
Plasmid#171241PurposeMammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser81Ala (AR-S81A)DepositorInsert3xFlag-tagged human androgen receptor Ser81Ala mutant (AR Human)
UseTagsFlagExpressionMammalianMutationAR-Ser81Ala (AR-S81A)PromoterCMVAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 myc-Rab10
Plasmid#208366PurposeMammalian expression of myc-tagged human Rab10DepositorInsertmyc-Rab10 (RAB10 Human)
UseTagsExpressionMammalianMutationwild typePromoterAvailable sinceDec. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
MBP-FUS_LC_12E
Plasmid#98654PurposeExpresses MBP-tagged phosphomimetic FUS AA 1-163DepositorInsertFUS (FUS Human)
UseTags6xHis-MBPExpressionBacterialMutation12 S/T to EPromoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DCX(S306D)-2A-mCherry
Plasmid#118292PurposeExpresses human mutant DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Serine at position 306 substituted for aspartic acidDepositorInsertDoublecortin (DCX Human)
UseAAVTags2A peptide and mCherryExpressionMammalianMutationSerine 306 changed to Aspartic AcidPromoterCMV with beta global intronAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DCX(S306A)-2A-mCherry
Plasmid#118291PurposeExpresses human mutant DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Serine at position 306 substituted for alanineDepositorInsertDoublecortin (DCX Human)
UseAAVTags2A peptide and mCherryExpressionMammalianMutationchanged Serine 306 to AlaninePromoterCMV with beta global intronAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX304-MARK3
Plasmid#107235PurposeLentiviral gene expression vector for phospho-MARK3 expressionDepositorInsertMARK3
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-ZIP(WT)
Plasmid#27134DepositorUseLentiviralTagseGFP and mCherryExpressionMammalianMutationPromoterAvailable sinceApril 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NMHC-IIA-3xA
Plasmid#101041PurposeExpresses GFP-MYH9 construct (human myosin IIA) carrying S1943A mutation and S1916A, S1915A mutation, driven by CMV promoter. Thus inhibits CKII and PKC phosphorylation of the tail.DepositorInsertNon-muscle myosin IIA heavy chain (MYH9 Human)
UseTagsGFPExpressionMammalianMutationS1915A, S1916A, S1943APromoterCMVAvailable sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xHA-MKK4-EE
Plasmid#47577PurposeBacterial expression plasmid for GST-3xHA-tagged constitutively active MKK4DepositorInsertMKK4-EE (MAP2K4 Human)
UseTags3xHA and GSTExpressionBacterialMutationLacks the first 36 residues of the RefSeq protein…PromoterAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLX304-MARK3_T211A
Plasmid#107236PurposeLentiviral gene expression vector for phospho-MARK3_T221A expressionDepositorInsertMARK3
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag-M4-AR-S81D
Plasmid#171242PurposeMammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser81Asp (AR-S81D)DepositorInsert3xFlag-tagged human androgen receptor Ser81Asp mutant (AR Human)
UseTagsFlagExpressionMammalianMutationAR-Ser81Asp (AR-S81D)PromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-EGFP-CLASP2 340-1362 9xS/A
Plasmid#24373DepositorInsertCLASP2 (340-1362) 9xS/A (CLASP2 Human)
UseTagsEGFPExpressionMammalianMutationNonphosphorylatable CLASP2 deletion mutant. M…PromoterAvailable sinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-AR-S81E
Plasmid#171224PurposeMammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser81Glu (AR-S81E)DepositorInserthuman androgen receptor Ser81Glu mutant (AR Human)
UseTagsExpressionBacterial and MammalianMutationAR-Ser81Glu (AR-S81E)PromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAdEasy-EGFP-CLASP2 340-1084 9xS/A
Plasmid#24525DepositorInsertCLASP2 (340-1084) 9xS/A (CLASP2 Human)
UseAdenoviralTagsEGFPExpressionMammalianMutationNonphosphorylatable CLASP2 deletion mutant. M…PromoterAvailable sinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only