We narrowed to 5,007 results for: puc

1. pUC19-link-mVenus-FLAG:Kan

   Plasmid

   #211830

   Purpose

   Plasmid template that encodes the fluorescent protein mVenus with a FLAG tag next to kanamycin resistance gene. This template was used to copy out mVenus-FLAG-Kan for genetic tagging.

   Depositor

   Peng Chen

   Insert

   mVenus-FLAG-Kan
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   NA

   Promoter

   Available since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC-DTA-DEST-R3R4(R)

   Plasmid

   #141011

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   DTA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19.TRIM28.FKBP.knock-in.mRuby2

   Plasmid

   #185008

   Purpose

   For knocking in FKBP F36V P2A mRuby2

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   FKBP F36V P2A mRuby2
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC18T-mini-Tn7T-Gm-Pc-mKate2

   Plasmid

   #199249

   Purpose

   Fluorescent labelling of bacterial cells with mKate2

   Depositor

   Uwe Mamat

   Article

   Mamat et al Appl Environ Microbiol. 2023 May 17:e003

   Insert

   Codon-optimized mKate2 gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimization for Xanthomonadaceae

   Promoter

   Pc promoter from class III integron of Delftia a…

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC18-mini-Tn7T-Gm-LacZ20

   Plasmid

   #65027

   Purpose

   GmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 2. See Depositor Comments below for information regarding the lacZ sequence.

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Protoc. 2006;1(1):153-61.

   Type

   Empty backbone

   Use

   Tags

   lacZ

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 2, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pUC118-mCtnnb1-FLIP-Puro

   Plasmid

   #84540

   Purpose

   CRISPR - FLIP targeting vector for mouse beta-catenin

   Depositor

   Bon-Kyoung Koo

   Article

   Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

   Insert

   mouse beta-catenin FLIP - Puro targeting vector
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC18T-mini-Tn7T-hph

   Plasmid

   #218366

   Purpose

   Empty backbone for Tn7-site insertion

   Depositor

   Michael Gebhardt

   Article

   Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19-URA3-3'SEC13-GFP

   Plasmid

   #25445

   Depositor

   Benjamin Glick

   Article

   Rossanese et al J Cell Biol. 1999 Apr 5. 145(1):69-81.

   Insert

   Sec13-Green Fluorescent Protein
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC-UAS1B8-TEF(504)

   Plasmid

   #44372

   Depositor

   Hal Alper

   Article

   Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

   Insert

   UAS1B8-TEF(504) promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   UAS1B8-TEF(504)

   Available since

   July 15, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1

    Plasmid

    #98972

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 1 and Cas9(D10A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC18T-mini-Tn7T-Gm-REP

    Plasmid

    #64966

    Purpose

    mini-Tn7 vector with oriR6K, for attTn7 site determination

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Insert

    oriR6K
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 14, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2

    Plasmid

    #98973

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 96bp 5' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 2 and Cas9(D10A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. p1977 (pUC19-PTDH3-PTEF1)

    Plasmid

    #78230

    Purpose

    Example plasmid containing a bidirectional promoter of pTDH3 and pTEF1

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    Bidirectional promoters pTDH3-pTEF1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pUC19.TRIM28.FKBP.knock-in.mCerulean

    Plasmid

    #185009

    Purpose

    For knocking in FKBP F36V P2A mCerulean

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    FKBP F36V P2A mCerulean
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC19-link-PAmCherry-FLAG:cat

    Plasmid

    #211832

    Purpose

    Plasmid template that encodes the fluorescent protein PAmCherry1 with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out PAmcherry1-FLAG-cat for genetic tagging.

    Depositor

    Peng Chen

    Insert

    PAmCherry-FLAG-cat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    NA

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19, containing H2-Kd Balb/c WK10

    Plasmid

    #102642

    Purpose

    Encodes sequence of the murine H2-Kd allele

    Depositor

    Sai Reddy

    Article

    Kelton et al Sci Rep. 2017 Apr 4;7:45775. doi: 10.103

    Insert

    H2-Kd Balb/c
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC-H1-Esp3I-mU6-Kan

    Plasmid

    #213015

    Purpose

    Vector for Cloning of multiple gRNAs driven by distinct promoters (H1 and mU6)

    Depositor

    Stephanie Roessler

    Insert

    H1 promoter, mU6 promoter, gRNA scaffolds
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    H1 promoter

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC-UAS1B16-TEF(203)

    Plasmid

    #44377

    Depositor

    Hal Alper

    Article

    Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

    Insert

    UAS1B16-TEF(203) promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    UAS1B16-TEF(203)

    Available since

    April 16, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pUC19-link-PAGFP-FLAG:Kan

    Plasmid

    #211834

    Purpose

    Plasmid template that encodes the fluorescent protein PAGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out PAGFP-FLAG-Kan for genetic tagging.

    Depositor

    Peng Chen

    Insert

    PAGFP-FLAG-Kan
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    NA

    Promoter

    Available since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC19-TSV (linearized in VP2)

    Plasmid

    #70032

    Purpose

    pUC19-TSV (linearized in VP2) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the VP2-coding region

    Depositor

    Mariet Feltkamp

    Article

    van der Meijden et al PLoS Pathog. 2010 Jul 29;6(7):e1001024.

    Insert

    TSV full genome, linearized in VP2 gene
    

    Use

    Cloning vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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