We narrowed to 3,093 results for: try
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Plasmid#162745PurposeNFAT-driven ZsGreen-1 reporter gene with mutated human CD4 geneDepositorInsert8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (CD4 Human)
UseRetroviralExpressionMammalianMutationHuman CD4 gene with glutamine to tyrosine at posi…Available sinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
W45A mutant of H3K9me3 biosensor
Plasmid#120808PurposeFRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capabilityDepositorInsertTruncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
ExpressionMammalianMutationTryptophan 45 is mutated to Alanine 45 on HP1 dom…PromoterCMVAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFP.E222
Plasmid#138248PurposeThree input / three output logic circuit plasmid - Expresses different intein split ECFs from different inducible promotersDepositorInsertsaraC (araC )
ECF20.N.(VDA).M86.N2
Terminator
SspGyrB.C2.(SAK).ECF16.C
Terminator
rhaS
ECF17.N.(NPC).NrdJ-1.N2
Terminator
M86.C2.(SDL).ECF20.C
Terminator
luxR
Terminator
ECF16.N.(AGG).SspGyrB.N2
Terminator
NrdJ-1.C2.(SEI).ECF17.C
UseSynthetic BiologyExpressionBacterialPromoterJ23115, P(araBAD), P(lux2), P(rhaBAD), iJ23101, a…Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-J23110-B0034-mRFP1E
Plasmid#160442PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1 chromoprotein in E. coliDepositorInsertpromoter, RBS, mRFP1
UseSynthetic BiologyMutationBioBrick sites removedAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pISceI Dusp6:memGFP
Plasmid#32668PurposeExpresses membrane localized EGFP under the Dusp6 promoterDepositorInsertDusp6 promoter (dusp6 Zebrafish)
UseZebrafish transgenesisAvailable sinceNov. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDONR207 SARS-CoV-2 NSP3 Cys857Ala
Plasmid#154027PurposeGateway-compatible Entry vectorDepositorInsertNSP3
UseGateway-compatible entry vector, with mutagenized…MutationMany synonymous changes due to codon optimizationAvailable sinceJune 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGL3-MMLV-LTR-Luc
Plasmid#67831PurposeExpression of luciferase under the control of MMLV viral LTRsDepositorInsertMMLV-LTR + primer binding site
UseLuciferaseTagsluciferaseExpressionMammalianAvailable sinceSept. 1, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSR83
Plasmid#90211PurposeExpresses the response regulator OmpR, and expresses mCherry from the Cph8-OmpR regulated PompF-146 promoterDepositorAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC-PTP-NEO
Plasmid#51710Purposecontains a C-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.DepositorTypeEmpty backboneUseT. brucei genome integration vectorsTagsPTP (ProtC-TEV-ProtA)Available sinceMarch 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMX_mTAF3
Plasmid#44334DepositorAvailable sinceMay 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
RIP7-RLuc-YFP-pBS
Plasmid#72482PurposeExpresses a renilla luciferase-YFP fusion protein under control of the rat insulin 2 gene promoter (RIP7) suitable for transfection or generation of transgenic animalsDepositorInsertsUseLuciferaseTagseYFP and renilla luciferaseExpressionMammalianMutationeYFP varientAvailable sinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAP03-ermE*p
Plasmid#120234PurposeCarries promoter cassette containing ermE*p promoter and ampicillin resistance gene that can be amplified with homology sequence for refactoring.DepositorInsertpromoter cassette containing ermE*p promoter and ampicillin resistance gene
UseSynthetic BiologyMutationbla (amp resistance gene)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pME-mVenus-CAAX
Plasmid#75153PurposeMembrane targeted mVenus (yellow) fluorophore for use with 5’ promoters.DepositorPublicationInsertmVenus-CAAX
UseMiddle entry vector for multisite gateway three f…Promotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-MyosinIIC-N-18
Plasmid#55108PurposeLocalization: Cytoskeleton, Excitation: 587, Emission: 610DepositorInsertMyosinIIC
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
YWHAG
Plasmid#39129PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAT-WT-HA-GFP11
Plasmid#177719PurposeER protein alpha-1-antitrypsin (WT) fused to an HA tag and the eleventh β-strand of GFPDepositorAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ239
Plasmid#108859PurposeFnCpf1 Gateway entry plasmidDepositorInsertFnCpf1
UseCRISPR; Gateway compatible cpf1 entry cloneTagsNLSExpressionPlantMutationCpf1 is rice codon optimizedAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MXS_PGK::CreERT2-bGHpA
Plasmid#62444PurposeMXS_chaining vector with PGK::CreERT2-bGHpADepositorInsertcassette containing the tamoxifen inducible Cre recombinase with PGK Promoter
UseSynthetic BiologyAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMtBS
Plasmid#32226DepositorInsertMCV Small t antigen
ExpressionMammalianMutationSplice site was silently mutated, and a restricti…Available sinceOct. 19, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENTR L3-mCherry-HA-L2
Plasmid#32313DepositorInsertmCherry-HA
UseGateway multisite entry vectorTagsHAAvailable sinceSept. 15, 2011AvailabilityAcademic Institutions and Nonprofits only