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pCORE-Hp53

Plasmid

#72242

Purpose

Contains the CORE cassette with hyg and human p53 gene markers

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Genet Eng (N Y). 2003;25:189-207.

Inserts

p53 (TP53 Human)
hyg

Expression

Bacterial

Mutation

V122A mutation

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pPEE17

Plasmid

#128361

Purpose

Detecting gene essentiality in Cryptococcus neoformans. Targets DNA constructs to the Safe Haven Site 1, a small gene-free region. Contains amdS2 counterselectable marker. This version of the Safe Haven vector includes direct repeats, enabling the formation of popouts on fluoroacetamide.

Depositor

James Fraser

Article

Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

Insert

Acetamidase (amdS)

Use

Unspecified

Promoter

TEF1

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pDYU1G/mRuby3–histone H1; γ-tubulin-mEGFP

Plasmid

#154303

Purpose

Expresses mRuby3–histone H1 and γ-tubulin-mEGFP from actin15 promoter in Dictyostelium discoideum

Depositor

Shigehiko Yumura

Article

Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

Inserts

histone H1
γ-tubulin

Use

Dictyostelium expression

Tags

mEGFP and mRuby3

Promoter

actin15

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR - DDX3Y sgRNA 2

Plasmid

#70657

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

sgRNA against DDX3Y

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only
Syt1LeftArm-3XGS-GCaMP6s-SV40-3XP3-dsRed-SV40-Syt1RightArm

Plasmid

#159636

Purpose

Donor plasmid for generating Syt1:GCaMP6s strain in Aedes aegypti with CRISPR/Cas9

Depositor

Carolyn McBride

Article

Zhao et al Cell Rep Methods. 2021 Jul 26;1(3):10004

Insert

GCaMP6s

Expression

Insect

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR - DDX3Y sgRNA 1

Plasmid

#70656

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

sgRNA against DDX3Y

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only
PB-Pitx2

Plasmid

#60668

Purpose

PiggyBac expression vector encoding murine Pitx2 downstream of a doxycycline inducible promoter

Depositor

James Thomson

Article

Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

Insert

Pitx2 (Pitx2 Mouse)

Use

Piggybac

Expression

Mammalian

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pKH-MoHyPer

Plasmid

#78267

Purpose

Express HyPer-AS sensor that change excitation wavelength with the presence of hydrogen peroxide.

Depositor

Jeffrey Caplan, Nicole Donofrio

Article

Huang et al Mol Plant Pathol. 2016 Mar 7. doi: 10.11

Insert

MoHyper

Use

Express in magnaporthe

Expression

Yeast

Promoter

Rp27

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only
pCDH_ss-SBP-EGFP

Plasmid

#172358

Purpose

Reporter to monitoring a streptavidin-binding peptide trafficking

Depositor

Guido Kroemer

Article

Zhao et al Sci Rep. 2018 Oct 8;8(1):14966. doi: 10.

Insert

ER importing signal sequence

Use

Lentiviral

Tags

SBP-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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p6993 MSCV-Neo C-HA 18E7

Plasmid

#163311

Purpose

Expresses HPV18 E7

Depositor

Elizabeth White

Article

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Insert

HPV18 E7

Use

Retroviral

Tags

HA

Promoter

MSCV LTR

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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p8126 MSCV-P C-FlagHA 16E7 R77S

Plasmid

#163305

Purpose

Expresses HPV16 E7 R77S

Depositor

Elizabeth White

Article

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Insert

HPV16 E7 R77S

Use

Retroviral

Tags

FlagHA

Mutation

R77S

Promoter

MSCV LTR

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pPI460

Plasmid

#113204

Purpose

Inducible expression vector (dox-on), C-terminal mScarlet tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum inducible expression vec…

Tags

mScarlet

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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NmMetQ WT

Plasmid

#129647

Purpose

Expresses the mature metQ gene from Neisseria meningitides, without the signal sequence

Depositor

Douglas Rees

Article

Nguyen et al Protein Sci. 2019 Jul 26. doi: 10.1002/p

Insert

metQ Gene

Tags

10x Histidine Tag, Enterokinase, and pelB signal …

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pPV230.13

Plasmid

#14902

Depositor

James Lok

Article

Li et al Int J Parasitol. 2006 May 31. 36(6):671-

Insert

Strongyloides stercoralis transitional endoplasmic reticulum ATPase promoter region

Expression

Worm

Mutation

Ss era-1 promoter region as a HindIII-PstI fragme…

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pMB38-HF380

Plasmid

#37332

Depositor

Takahide Kon

Article

Kon et al Nature. 2012 Mar 7;484(7394):345-50. doi

Insert

Cytoplasmic Dynein Heavy Chain (dhcA Dictyostelium discoideum)

Use

Dictyostelium expression

Tags

His6 and FLAG tag

Mutation

deleted amino acids 1-1387

Promoter

TRE-Pmin

Available Since

Aug. 22, 2012

Availability

Academic Institutions and Nonprofits only
pOPTXLUC

Plasmid

#68504

Purpose

cyclic nucleotide reporter for bacteria or plant cells

Depositor

Helen Irving

Article

Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

Insert

OPTX promoter (AT1G33440 Mustard Weed)

Tags

luciferase

Expression

Bacterial and Plant

Promoter

OPTX

Available Since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only

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p8141 MSCV-P C-FlagHA 18E7 D10K

Plasmid

#163306

Purpose

Expresses HPV18 E7 D10K

Depositor

Elizabeth White

Article

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Insert

HPV18 E7 D10K

Use

Retroviral

Tags

FlagHA

Mutation

D10K

Promoter

MSCV LTR

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pOVC1_RsLP-LOV_X

Plasmid

#131177

Purpose

Expresses RsLP-LOV (Rhodobacter sphaeroides) followed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

RsLP-LOV

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot2

Plasmid

#79255

Purpose

Expresses EGFP-tagged DmNot2 in S2 cells

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmNot2 (Rga Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Promoter

Ac5 promoter

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot3

Plasmid

#79250

Purpose

Expresses EGFP-tagged DmNot3 in S2 cells

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmNot3 (Not3 Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Promoter

Ac5 promoter

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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