We narrowed to 19,051 results for: Tec

1. pRDB_051

   Plasmid

   #216081

   Purpose

   for stable fly cell lines; attL and attR sequences for genome integration by phiC31, destination vector

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   attL and attR sequences for genome integration by phiC31
   

   Use

   Destination

   Expression

   Insect

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. AA151

   Plasmid

   #215938

   Purpose

   Fragmid fragment: (guide cassette) for dual expression sgRNA

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; BsmBI_v0; BsmBI_v4; rev{H1_v1}
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AA375

   Plasmid

   #216054

   Purpose

   Fragmid fragment: (C' terminus) HIV nuclear export sequence; HA tag

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   HIV-NES_v1.1; HIV-NES_v1.2; 3xHA_v1.1
   

   Use

   Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AA400

   Plasmid

   #215958

   Purpose

   Fragmid fragment: (guide cassette) guide expression

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; DR_v1 [Rfx]; BsmBI_v1; BsmBI_v12
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. AA401

   Plasmid

   #215959

   Purpose

   Fragmid fragment: (guide cassette) guide expression

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; DR_v1 [Dj]; BsmBI_v1; BsmBI_v12
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AA048

   Plasmid

   #216016

   Purpose

   Fragmid fragment: (Cas protein) deactivated Cas

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   dCas12a (D908A)_v1.1 [EnAs]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRDB_131

   Plasmid

   #216095

   Purpose

   Cas12a [EnAs] CRISPRa targeting CD97, CD4, CD26, CD274, positive control

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   CD97, CD4, CD26, CD274 guides
   

   Use

   CRISPR and Lentiviral; Positive controls

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRDA_816

   Plasmid

   #216098

   Purpose

   CRISPRa, EF1a-driven ALFA-dCas12a-VP64 (Cas only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   Cas12a [EnAs]
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPAP_scFv

   Plasmid

   #183733

   Purpose

   Expresses anti-lysozyme scFv antibody

   Depositor

   Jun Ishii

   Article

   Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

   Insert

   Anti-lysozyme scFv
   

   Tags

   His6-tag

   Expression

   Yeast

   Promoter

   AOX1 promoter

   Available Since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPR/eCas9-GPIDAF

    Plasmid

    #213710

    Purpose

    To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIDAF under separate promoters.

    Depositor

    Qilai Huang

    Article

    Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    3XFLAG and Twin-strep-tag

    Promoter

    CMV

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPR/eCas9-GPIBY55

    Plasmid

    #213709

    Purpose

    To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIBY55 under separate promoters.

    Depositor

    Qilai Huang

    Article

    Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    3XFLAG and Twin-strep-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. sfGFP-SasG(3G)-mCherry

    Plasmid

    #206945

    Purpose

    Mammalian expression of heterobifunctional SasG linkers (3G)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    sfGFP-SaSasG (3G)-mCherry
    

    Tags

    mCherry and sfGFP

    Expression

    Mammalian

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLINE1ΔORF2-scFvFc

    Plasmid

    #206021

    Purpose

    The ORF2 sequence was removed from pLINE1-scFvFc

    Depositor

    Masamichi Kamihira

    Article

    Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

    Insert

    mouse LINE1 vector harboring an scFv-Fc expression unit, lacking the ORF2 sequence
    

    Expression

    Mammalian

    Available Since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLINE1ΔORF1-2-scFvFc

    Plasmid

    #206022

    Purpose

    The ORF1 and ORF2 sequences were removed from pLINE1-scFvFc

    Depositor

    Masamichi Kamihira

    Article

    Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

    Insert

    mouse LINE1 vector harboring an scFv-Fc expression unit, lacking the ORF1 and ORF2 sequences
    

    Expression

    Mammalian

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. PET-HypaSpCas9-NLS-6xHis

    Plasmid

    #207384

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HypaSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N692A, M694A, Q695A, H698A

    Promoter

    T7

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. sfGFP-SasG(7G)-mCherry

    Plasmid

    #206949

    Purpose

    Mammalian expression of heterobifunctional SasG linkers (7G)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    sfGFP-SaSasG (7G)-mCherry
    

    Tags

    mCherry and sfGFP

    Expression

    Mammalian

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. sfGFP-SasG(6G)-mCherry

    Plasmid

    #206948

    Purpose

    Mammalian expression of heterobifunctional SasG linkers (6G)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    sfGFP-SaSasG (6G)-mCherry
    

    Tags

    mCherry and sfGFP

    Expression

    Mammalian

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. sfGFP-SasG(5G)-mCherry

    Plasmid

    #206947

    Purpose

    Mammalian expression of heterobifunctional SasG linkers (5G)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    sfGFP-SaSasG (5G)-mCherry
    

    Tags

    mCherry and sfGFP

    Expression

    Mammalian

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. sfGFP-SasG(4G)-mCherry

    Plasmid

    #206946

    Purpose

    Mammalian expression of heterobifunctional SasG linkers (4G)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    sfGFP-SaSasG (4G)-mCherry
    

    Tags

    mCherry and sfGFP

    Expression

    Mammalian

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. sfGFP-SasG(2G)-mCherry

    Plasmid

    #206944

    Purpose

    Mammalian expression of heterobifunctional SasG linkers (2G)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    sfGFP-SaSasG (2G)-mCherry
    

    Tags

    mCherry and sfGFP

    Expression

    Mammalian

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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