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hp-50-0-50%GC-Right-half-120bp

Plasmid

#169207

Purpose

50-0-50%GC-Right-half-120bp-hairpin

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

50-0-50%GC-Right-half-120bp-hairpin

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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hp-50%GC-20bp

Plasmid

#169196

Purpose

Force-activating hairpin for 3'-to-5' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

50%GC-20bp-hairpin

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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hp-50%GC-40bp

Plasmid

#169197

Purpose

Force-activating hairpin for 3'-to-5' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

50%GC-40bp-hairpin

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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hp-50%GC-40bp-Revbindsite

Plasmid

#169198

Purpose

Force-activating hairpin for 5'-to-3' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

50%GC-40bp-hairpin-binding-site-reversed

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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hp-66%GC-20bp

Plasmid

#169199

Purpose

Force-activating hairpin for 3'-to-5' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

66%GC-20bp-hairpin

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGE010-sgRNA-Target1 (MpRTN1)

Plasmid

#186295

Purpose

Binary vector for CRISPR/Cas9 (target 1: MpRTN1) in plants (for Agrobacterium-mediated genetic transformation)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

sgRNA-Target1

Use

CRISPR

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-mMOV10-3'UTR

Plasmid

#178905

Purpose

dual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicase

Depositor

Constance Ciaudo

Article

Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

Insert

3'UTR mMov10 (Mov10 Mouse)

Use

Luciferase

Promoter

SV40 promoter

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-T11HA-hMOV10-WT

Plasmid

#178907

Purpose

expression of WT hMOV10

Depositor

Constance Ciaudo

Article

Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

Insert

cDNA WT hMOV10 (MOV10 Human)

Tags

T11 beta strand of GFP-HA

Expression

Mammalian

Promoter

CMV promoter

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9355(II-1_Markerfree_BackBone)

Plasmid

#161597

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site II-1, (Chr II: 341622..341641)

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9356(IV-1_Markerfree_BackBone)

Plasmid

#161598

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site IV-1, (Chr IV: 132921..132940)

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9360(IX-1_Markerfree_BackBone)

Plasmid

#161601

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site IX-1, (Chr IX: 388864..388883)

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9362(XV-1_Markerfree_BackBone)

Plasmid

#161603

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XV-1, (Chr XV: 93186..93205)

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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TalO8-HSP30-CFP

Plasmid

#178304

Purpose

CoTrIP plasmid with HSP30 promoter driving CFP's expression

Depositor

Brian Zid

Article

Chen et al eLife. 2022 Sep 15;11. pii: 76965. doi:

Insert

HSP30pr-CFP-tAdh1

Expression

Yeast

Promoter

HSP30 and NA

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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TalO8-HSP26-CFP

Plasmid

#178306

Purpose

CoTrIP plasmid with HSP26 promoter driving CFP's expression

Depositor

Brian Zid

Article

Chen et al eLife. 2022 Sep 15;11. pii: 76965. doi:

Insert

HSP26pr-CFP-tAdh1

Expression

Yeast

Promoter

HSP26 and NA

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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TalO8-GLC3-CFP

Plasmid

#178307

Purpose

CoTrIP plasmid with GLC3 promoter driving CFP's expression

Depositor

Brian Zid

Article

Chen et al eLife. 2022 Sep 15;11. pii: 76965. doi:

Insert

Glc3pr-CFP-tAdh1

Expression

Yeast

Promoter

GLC3 and NA

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-DmDCP2-E361Q-dsRNAres-V5His6_J

Plasmid

#146689

Purpose

Insect Expression of DmDCP2-E361Q-dsRNAres

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmDCP2-E361Q-dsRNAres (DCP2 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR

Plasmid

#186276

Purpose

Plasmid containing shortened version of upstream Thiomicrospira CRISPR array for integration assays

Depositor

Jennifer Doudna

Article

Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

Insert

Thiomicrospira shortened CRISPR array

Expression

Bacterial

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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Paxillin DN

Plasmid

#186151

Purpose

For expressing recombinant paxillin variants in bacteria.

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PXN (PXN Human)

Tags

GST

Expression

Bacterial

Mutation

Paxilln residues 1-321 added to N-terminus of WT …

Promoter

Lac

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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p8130 MSCV GFP Puro C-FlagHA

Plasmid

#184521

Purpose

Expression of GFP

Depositor

Elizabeth White

Article

Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

Insert

GFP

Use

Retroviral

Tags

Flag, HA

Promoter

MSCV LTR

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pIVT-AIDm-EGFP

Plasmid

#182044

Purpose

in vitro transcription of AIDm-EGFP

Depositor

Janice Evans

Article

Camlin et al Biol Reprod. 2019 Oct 25;101(4):704-718.

Insert

AIDm-EGFP

Use

In vitro transcription

Tags

EGFP

Available Since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

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