We narrowed to 10,238 results for: ada

1. MSCV-HA-Nr4a1-AA

   Plasmid

   #160942

   Purpose

   Generation of retrovirus for the overexpression of Nr4a1 mutant (287C 288EAA)

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Nr4a1 (Nr4a1 Mouse)
   

   Use

   Retroviral

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Change Cys 287 Glu 288 into Ala Ala

   Promoter

   MSCV-LTR

   Available Since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB-cT3G-cERP2-EMX2

   Plasmid

   #192898

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of EMX2

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   EMX2 (EMX2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV_SEPT9_i1-14-GFP10

   Plasmid

   #180343

   Purpose

   mammalian expression of human SEPT9_i1 fused to GFP10

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Insert

   SEPTIN9_v1 (SEPTIN9 Human)
   

   Tags

   GFP10

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKK-RNAi-nucEGFPmiR-mCherry-TEV

   Plasmid

   #105808

   Purpose

   Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with N-terminal mCherry.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   RNAi; Flp-in competent

   Tags

   mCherry-TEV

   Expression

   Mammalian

   Available Since

   May 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMXS-IRES-Blast POLE1 C-ISCmut

   Plasmid

   #160804

   Purpose

   Express C-terminal ISC mutant POLE1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   POLE1 C-ISCmut (POLE Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Mutation

   shPOLE_1 and shPOLE2 resistant, C2221S, C2224S, C…

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXJ42-p110 CUX1

   Plasmid

   #100814

   Purpose

   Plasmid vector expressing human p110 CUX1 (amino acids 878-1505 of P39880), with an N-terminal Myc tag and a C-terminal HA tag.

   Depositor

   Alain Nepveu

   Article

   Truscott et al Mol Cell Biol. 2008 May;28(10):3127-38.

   Insert

   p110 CUX1 (amino acids 878-1505) (CUX1 Human)
   

   Tags

   HA and Myc

   Expression

   Mammalian

   Mutation

   Contains amino acids 878-1505

   Available Since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKK-RNAi-nucEGFPmiR-FLAG-TEV

   Plasmid

   #105807

   Purpose

   Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with N-terminal FLAG tag.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   RNAi; Flp-in competent

   Tags

   FLAG-TEV

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCW-ND-Cdt1-HA-Puro

   Plasmid

   #193767

   Purpose

   Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal HA tag) with mutations which abroggate its S phase degradation, expressed from all-in-one pCW vector (TetOn + rtTA)

   Depositor

   Tobias Meyer

   Article

   Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

   Insert

   ND-Cdt1-HA (CDT1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…

   Promoter

   TRE

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB-cT3G-cERP2-JUN

   Plasmid

   #192895

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of JUN

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   JUN (JUN Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSPL3-ABCC8-27

    Plasmid

    #135919

    Purpose

    Encodes ABCC8 exon 27 wild type for the analysis of splicing variants

    Depositor

    Diana Valverde Pérez

    Article

    Lago-Docampo et al Sci Rep. 2020 Sep 15;10(1):15135. doi: 1

    Insert

    ATP-binding Cassette subfamily C member 8 (ABCC8 Human)
    

    Use

    Exon trapping

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL3-noSV40-humanEC1.45_p300peak1

    Plasmid

    #173947

    Purpose

    Firefly luciferase enhancer reporter plasmid.

    Depositor

    Joanna Wysocka

    Article

    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

    Insert

    p300 peak 1 from SOX9 enhancer cluster EC1.45
    

    Use

    Luciferase

    Mutation

    None

    Promoter

    SV40 promoter

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. PLL5.0-Anillin ShRNA-Cherry-AnillinA740D, E758K

    Plasmid

    #187273

    Purpose

    Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin A740D,E758K

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Mutation

    Anillin A740D, E758K

    Promoter

    U6, CMV

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA 3.1 SIRT1-deltaExon2

    Plasmid

    #105671

    Purpose

    Mammalian expression construct of mouse SIRT1 short isoform (lacking Exon2)

    Depositor

    Ullas Kolthur-Seetharam

    Article

    Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

    Insert

    SIRT1 (Sirt1 Mouse)
    

    Expression

    Mammalian

    Mutation

    Synonymous base changes not affecting the protein…

    Promoter

    CMV

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. Flag-M4-AR-W741C-S650A

    Plasmid

    #171252

    Purpose

    Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser650Ala (AR-W741C-S650A)

    Depositor

    Steven Balk

    Article

    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

    Insert

    3xFlag-tagged human androgen receptor Trp741Cys-Ser650Ala mutant (AR Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AR-Trp741Cys-Ser650Ala (AR-W741C-S650A)

    Promoter

    CMV

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Flag-M4-AR-W741C-S650D

    Plasmid

    #171253

    Purpose

    Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser650Asp (AR-W741C-S650D)

    Depositor

    Steven Balk

    Article

    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

    Insert

    3xFlag-tagged human androgen receptor Trp741Cys-Ser650Asp mutant (AR Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AR-Trp741Cys-Ser650Asp (AR-W741C-S650D)

    Promoter

    CMV

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKK-RNAi-nucCHERRYmiR-TEV-FLAG

    Plasmid

    #105813

    Purpose

    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with C-terminal FLAG.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    RNAi; Flp-in competent

    Tags

    TEV-FLAG

    Expression

    Mammalian

    Available Since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-eGFP-NLS-RNF168 delta RING

    Plasmid

    #133983

    Purpose

    mammalian expression vector of eGFP tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)

    Depositor

    Daniel Durocher

    Article

    Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

    Insert

    RNF168 (RNF168 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    deletion of RING; siRNA resistant sequence: GAGGA…

    Promoter

    CMV

    Available Since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. MSCV-NSD3-short del(280-342)

    Plasmid

    #72550

    Purpose

    expresses 3*FLAG tagged human NSD3-short with 280-342 aa deletion

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    NSD3-short (NSD3 Human)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    280-342 aa deletion

    Promoter

    LTR

    Available Since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. W118-1_hRSK3(RPS6KA2)_K91A_K444A

    Plasmid

    #180385

    Purpose

    lentiviral transduction of human RSK3 (RPS6KA2) gene with point mutations (K91A and K444A) that inactivate kinase activity

    Depositor

    Huda Zoghbi

    Article

    Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

    Insert

    RPS6KA2_K91A/K444A (RPS6KA2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    K91A/K444A

    Promoter

    CMV

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL3-noSV40-mouseEC1.45

    Plasmid

    #173972

    Purpose

    Firefly luciferase enhancer reporter plasmid.

    Depositor

    Joanna Wysocka

    Article

    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

    Insert

    Mouse orthologous SOX9 enhancer cluster EC1.45
    

    Use

    Luciferase

    Mutation

    None

    Promoter

    SV40 promoter

    Available Since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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