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We narrowed to 8,836 results for: construct

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Showing: 7581 - 7600 of 8836 results

  3. AGTR2-DuET

    Plasmid
    #213184
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    AGTR2 (AGTR2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    213184-plasmid-map-sequence-id-431748

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  6. pHSN6I01

    Plasmid
    #50587
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    dCas9-KRAB
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Mutation
    dCas9 that is defective in DNA cleavage; the maiz…
    Promoter
    2×35Sp and AtU6-26p
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50587-plasmid-map-sequence-id-75659

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  7. MCHR2-DuET

    Plasmid
    #213338
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    MCHR2 (MCHR2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213338-plasmid-map-sequence-id-430867

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  8. pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA

    Plasmid
    #190790
    Purpose
    [E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.
    Depositor
    Paul Hudson
    Article
    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102
    Inserts
    Cas9
    mazF-LVA
    Use
    CRISPR and Synthetic Biology
    Tags
    LVA
    Expression
    Bacterial
    Mutation
    silent mutations of BpiI-sites in original Cas9 g…
    Promoter
    PnrsB and Ptrc-[E*](104)
    Available Since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    190790-plasmid-map-sequence-id-384200

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  9. OSC_Reporter_UAS_traffic jam_BoxB_d2eGFP_t2a_Blast

    Plasmid
    #128010
    Purpose
    traffic jam enhancer driven GFP_P2A-Blasticidin-resistance harboring 10 intronic boxB sites and 14 upstream UAS sites
    Depositor
    Julius Brennecke
    Article
    Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-
    Insert
    traffic jam enhancer driven GFP_P2A-Blasticidin-resistance harboring 10 intronic boxB sites and 14 upstream UAS sites
    Use
    Drosophila osc reporter construct
    Available Since
    July 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    128010-plasmid-map-sequence-id-251562

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  10. MtPT4-p3

    Plasmid
    #160003
    Purpose
    Multigene construct containing the betalain pigment biosynthetic genes under the control of the MtPT4 promoter for visualisation of arbuscular mycorrhizal colonisation in Medicago truncatula roots.
    Depositor
    Sam Brockington
    Article
    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d
    Inserts
    DsRed (reverse orientation)
    DODAα1
    CYP76AD1
    cDOPA5GT
    Expression
    Plant
    Promoter
    Arabidopsis thaliana Ubiquitin 10 Promoter (AtUb1…
    Available Since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    160003-plasmid-map-sequence-id-323050

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  11. FUW-crRNA1and2-U6_TRE-dLbCpf1-NLS-hCTCF_WT-HA-P2A-tagBFP

    Plasmid
    #194885
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-WT and crRNA-1 and -2 targeting the human MECP2 locus.
    Depositor
    Shawn Liu
    Article
    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd
    Inserts
    dLbCpf1-NLS-hCTCF_WT
    U6-crRNA1and2
    Use
    Lentiviral
    Tags
    HA
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Available Since
    Jan. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    194885-plasmid-map-sequence-id-382817

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  12. GHSR-DuET

    Plasmid
    #213251
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GHSR (GHSR Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213251-plasmid-map-sequence-id-430901

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  13. HTR2B-DuET

    Plasmid
    #213322
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HTR2B (HTR2B Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213322-plasmid-map-sequence-id-430858

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  15. p-mCherry-C1-iC++

    Plasmid
    #98165
    Purpose
    artificial anion conducting channelrhodopsin (aACR). Activation max 488 nm; off-kinetics 35 ms. Codon optimized for mammalian expression.
    Depositor
    Peter Hegemann
    Article
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Insert
    Synthetic construct iC++ gene
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    T59S, E83N, E90Q, E101S, V117R, E123S, V242R, T24…
    Promoter
    CMV (+enhancer)
    Available Since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98165-plasmid-map-sequence-id-182714

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  18. NbPT5b-p3

    Plasmid
    #160001
    Purpose
    Multigene construct containing the betalain pigment biosynthetic genes under the control of the NbPT5b promoter for visualisation of arbuscular mycorrhizal colonisation in Nicotiana benthamiana roots.
    Depositor
    Sam Brockington
    Article
    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d
    Inserts
    Bar (reverse orientation)
    DODAα1
    CYP76AD1
    cDOPA5GT
    Expression
    Plant
    Promoter
    Agrobacterium tumefaciens Nopaline synthase Promo…
    Available Since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    160001-plasmid-map-sequence-id-323057

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  19. C3AR1-DuET

    Plasmid
    #213191
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    C3AR1 (C3AR1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213191-plasmid-map-sequence-id-430756

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  20. C5A-DuET

    Plasmid
    #213192
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    C5A (C5AR1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213192-plasmid-map-sequence-id-430757

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Showing: 7581 - 7600 of 8836 results