161,492 results

1. pOCC5

   Plasmid

   #118863

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-3C-NotI-ccdB-AscI-stop-HindIII cassette
   

   Use

   Tags

   HIS6, cleavable with 3C protease

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-hSyncytin-1

   Plasmid

   #182440

   Purpose

   hSyncytin-1

   Depositor

   Masaharu Somiya

   Insert

   ERVW-1 (ERVW-1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNP2270

   Plasmid

   #222733

   Purpose

   pNP1950 - J23119-IS621 bRNA1 TBL-lacZ-1 (Extended RTG), DBL-WT, HDV; T7-IS621 Recombinase

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Insert

   IS621 Helper (lacZ)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFA6a-5FLAG-natMX6

   Plasmid

   #19343

   Depositor

   Eishi Noguchi

   Article

   Noguchi et al Biotechnol J. 2008 Aug 26. ():.

   Insert

   natMX6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL4.23-GW

   Plasmid

   #60323

   Purpose

   This is a modified version of the pGL4.23 vector from Promega, containing a Gateway cassette upstream of the minimal promoter. This vector can be used for for Gateway cloning of candidate enhancer elements. As negative control, use pGL4.23 without the gateway cassette (available from Promega).

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOpen_taq

   Plasmid

   #153315

   Purpose

   Bacterial expression of Taq polymerase

   Depositor

   Drew Endy

   Insert

   Taq polymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   June 2, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pBT277

   Plasmid

   #122608

   Purpose

   express evoCDA1-BE4max in mammalian cells

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   evoCDA1-BE4max
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   F23S A123V I195F

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV_BiVIPe4_dTomato_nlsdTomato

   Plasmid

   #213855

   Purpose

   AAV construct expressing dTomato driven by VIP interneuron-targeting enhancer. Alias: pAAV_YWE4.2_dTomato_nlsdTomato

   Depositor

   Gordon Fishell

   Article

   Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

   Has Service

   AAV PHP.eB

   Insert

   dTomato_P2A_nls.dTomato
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQE30-His-IF3

   Plasmid

   #124125

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Initiation factor 3 (infC Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBMN mEGFP-NDP52

    Plasmid

    #188785

    Purpose

    For mammalian expression of NDP52 tagged with mEGFP

    Depositor

    Michael Lazarou

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Retroviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV_BiSSTe4_dTomato_nlsdTomato

    Plasmid

    #213944

    Purpose

    AAV construct expressing dTomato driven by SST interneuron-targeting enhancer. Alias: AAV_WDS0004_dTomato_nlsdTomato

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Has Service

    AAV PHP.eB

    Insert

    dTomato_P2A_nls.dTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-JNK1

    Plasmid

    #86830

    Purpose

    mammalian expression of GFP-JNK1

    Depositor

    Rony Seger

    Article

    Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

    Insert

    JNK1 (MAPK8 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. PL-SIN-EF1α-EGFP

    Plasmid

    #21320

    Purpose

    Control Lentiviral vector with strong EF1a promoter, expresses EGFP

    Depositor

    James Ellis

    Article

    Hotta et al Nat Methods. 2009 May . 6(5):370-6.

    Insert

    Enhanced Green Fluorescence Protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    June 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-IDO1-p2a-eGFP

    Plasmid

    #187026

    Purpose

    Expresses IDO1 and GFP (seperately) in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Maassen et al Free Radic Biol Med. 2022 Aug 1;188:287-

    Insert

    Indoleamine 2,3-dioxygenase 1 (IDO1 Human)
    

    Use

    Tags

    P2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223_BFP

    Plasmid

    #25891

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Insert

    BFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJH376

    Plasmid

    #86842

    Purpose

    mamalian expression of anti-CRISPR protein AcrIIA4

    Depositor

    Joseph Bondy-Denomy

    Article

    Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

    Insert

    acrIIA4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pZS165 CRISPEY HH-HDV NotI

    Plasmid

    #114451

    Purpose

    Yeast Centromere Plasmid for galactose inducible expression of retron-guide chimeric RNA with flanking HH-HDV ribozyme

    Depositor

    Hunter Fraser

    Article

    Sharon et al Cell. 2018 Sep 18. pii: S0092-8674(18)31

    Type

    Empty backbone

    Use

    CRISPR; Cen/ars

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScGAL7p

    Available since

    Oct. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMVTNT PINK1 C-myc

    Plasmid

    #13314

    Depositor

    Mark Cookson

    Article

    Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

    Insert

    PINK1 (PINK1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pscALPSpuro-RsACE2

    Plasmid

    #159665

    Purpose

    Expresses Rhinolophus sinicus ACE2 in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    rhinolophus sinicus ACE2 (ACE2 rhinolophus sinicus)
    

    Use

    Lentiviral

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pExp-GST

    Plasmid

    #112566

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GST fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-GST and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge