Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 16,261 results for: LEA

Showing: 7661 - 7680 of 16261 results

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pLV-mEGFP-hCTNNA1_P-linker 4E

Plasmid

#234564

Purpose

CTNNA1 Phospholinker phospho-mimic

Depositor

Cara Gottardi

Article

Wang et al J Cell Biol. 2025 Mar 3;224(3):e20230812

Insert

mEGFP:hCTNNA1_P-linker 4E (CTNNA1 Human)

Use

Lentiviral

Tags

mEGFP

Mutation

S641E, S652E, S655E, T658E

Promoter

CMV

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-mEGFP-hCTNNA1_P-linker 4A

Plasmid

#234563

Purpose

CTNNA1 Phospholinker phospho-mutant

Depositor

Cara Gottardi

Article

Wang et al J Cell Biol. 2025 Mar 3;224(3):e20230812

Insert

mEGFP:hCTNNA1_P-linker 4A (CTNNA1 Human)

Use

Lentiviral

Tags

mEGFP

Mutation

S641A, S652A, S655A, T658A

Promoter

CMV

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-mEGFP-hCTNNA1_E307K

Plasmid

#234560

Purpose

CTNNA1 butterfly eye mutant

Depositor

Cara Gottardi

Article

Wang et al J Cell Biol. 2025 Mar 3;224(3):e20230812

Insert

mEGFP:hCTNNA1_E307K (CTNNA1 Human)

Use

Lentiviral

Tags

mEGFP

Mutation

E307K

Promoter

CMV

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-mEGFP-hCTNNA1_R551A

Plasmid

#234558

Purpose

CTNNA1 M2-M3 salt-bridge disrupting mutant

Depositor

Cara Gottardi

Article

Wang et al J Cell Biol. 2025 Mar 3;224(3):e20230812

Insert

mEGFP:hCTNNA1_R551A (CTNNA1 Human)

Use

Lentiviral

Tags

mEGFP

Mutation

R551A

Promoter

CMV

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-mEGFP-hCTNNA1_DNii domain

Plasmid

#234557

Purpose

CTNNA1 Nii-domain deletion mutant

Depositor

Cara Gottardi

Article

Wang et al J Cell Biol. 2025 Mar 3;224(3):e20230812

Insert

mEGFP:hCTNNA1_DNii domain (CTNNA1 Human)

Use

Lentiviral

Tags

mEGFP

Mutation

Deletion of aa ~165-265

Promoter

CMV

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-mEGFP-hCTNNA1_DM2,3-domain

Plasmid

#234556

Purpose

CTNNA1 M2,3-domain deletion mutant

Depositor

Cara Gottardi

Article

Wang et al J Cell Biol. 2025 Mar 3;224(3):e20230812

Insert

mEGFP:hCTNNA1_DM2,3-domain (CTNNA1 Human)

Use

Lentiviral

Tags

mEGFP

Mutation

Deletion of aa 397-509

Promoter

CMV

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-mEGFP-hCTNNA1_DM1-domain

Plasmid

#234555

Purpose

CTNNA1 M1-domain deletion mutant

Depositor

Cara Gottardi

Article

Wang et al J Cell Biol. 2025 Mar 3;224(3):e20230812

Insert

mEGFP:hCTNNA1_DM1-domain (CTNNA1 Human)

Use

Lentiviral

Tags

mEGFP

Mutation

Deletion of aa 271-396

Promoter

CMV

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-FLAG(N)-CDC73

Plasmid

#231751

Purpose

Expression in Flp-IN human cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDC73 (CDC73 Human)

Tags

Flag-3C

Expression

Mammalian

Mutation

siCDC73#6 and #8 resistant

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-FLAG(N)-CDC73deltaKIM

Plasmid

#231752

Purpose

Expression in Flp-IN human cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDC73 (CDC73 Human)

Tags

Flag-3C

Expression

Mammalian

Mutation

siCDC73#6 and #8 resistant; CDC73(delta290-324)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-FLAG(N)-CDC73-YY/AA

Plasmid

#231753

Purpose

Expression in Flp-IN human cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDC73 (CDC73 Human)

Tags

Flag-3C

Expression

Mammalian

Mutation

siCDC73#6 and #8 resistant; CDC73(Y290A, Y293A)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-FLAG(N)-CDK12deltaNTD(700-1490)

Plasmid

#231734

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDK12 (CDK12 Human)

Tags

Flag-3C

Expression

Insect

Mutation

CDK12(700-1490)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-FLAG(N)-CDK12-KiD(700-1082)

Plasmid

#231736

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDK12 (CDK12 Human)

Tags

Flag-3C

Expression

Insect

Mutation

CDK12(700-1082)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-FLAG(N)-CDK12deltaRS(delta104-380)

Plasmid

#231737

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDK12 (CDK12 Human)

Tags

Flag-3C

Expression

Insect

Mutation

CDK12(delta104-380)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-FLAG-GST(N)-CDK12RS(104-380)

Plasmid

#231738

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDK12 (CDK12 Human)

Tags

Flag-GST-3C

Expression

Insect

Mutation

CDK12(104-380)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-FLAG(N)-CDC73deltaKIM(delta290-324)

Plasmid

#231739

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDC73 (CDC73 Human)

Tags

Flag-3C

Expression

Insect

Mutation

CDC73(delta290-324)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-FLAG(N)-CDC73-YY/AA(Y290A, Y293A)

Plasmid

#231740

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDC73 (CDC73 Human)

Tags

Flag-3C

Expression

Insect

Mutation

CDC73(Y290A, Y293A)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-U6/TO-shEsr1-CMV-TetR-eGFP

Plasmid

#233298

Purpose

DOX-inducible U6 driven shRNA against mouse Esr1

Depositor

Dayu Lin

Article

Golden et al bioRxiv [Preprint]. 2024 Sep 25:2023.12.

Insert

Esr1 shRNA (Esr1 Mouse)

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-Luciferase-P2A-H2A-mCherry (JDW 1129)

Plasmid

#229823

Purpose

A CAGGS driven luciferase reporter followed by a P2A cleavage peptide and an H2A mCherry cassette for nuclear labeling.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Luciferase-P2A-H2A-mCherry

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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AP2u2-CMV-S_protein

Plasmid

#226456

Purpose

Mammalian expression of S protein of SARS-CoV2 under CMV promoter, tagged with 6His and released extracellular environment after expression for protein purification

Depositor

Urartu Seker

Article

Ozcelik et al ACS Pharmacol Transl Sci. 2024 Mar 21;7(

Insert

Spike Protein

Tags

6xHis Tag, Foldon Domain, and Thrombin Cleavage S…

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD-WzxE-TEV-His10

Plasmid

#226781

Purpose

Expression of C-terminally TEV cleavable His10-tagged WzxE from E. coli in E. coli

Depositor

James Naismith

Article

Le Bas et al Open Biol. 2025 Jan;15(1):240310. doi: 1

Insert

wzxE

Tags

TEV cleavable 10xHistidine tag

Expression

Bacterial

Mutation

valine insertion in position 2 due to the cloning…

Promoter

araBAD

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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Showing: 7661 - 7680 of 16261 results

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