We narrowed to 15,991 results for: LEA

1. pINTO-N3::hTET3

   Plasmid

   #136364

   Purpose

   Expresses epitope-tagged human TET3 WT in mammalian cells

   Depositor

   Roberto Bonasio

   Article

   Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

   Insert

   TET3 (TET3 Human)
   

   Tags

   2xStrep-tagII, FLAG, and HA

   Expression

   Mammalian

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-U6-BsmBI_epegRNA_entry-mpknot_(LM1140)

   Plasmid

   #228253

   Purpose

   pU6 entry plamid for cloning prime editor mpknot epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   mpknot entry plasmid backbone for epegRNA expression via human U6 promoter
   

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   U6

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pelB-MBP-HtrA-ss

   Plasmid

   #137066

   Purpose

   E. coli expression clone (T7lac promoter) for mature HtrA (aa 28-474) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAC66500.2 (BB_0104 Borrelia burgdorferi B31)
   

   Tags

   PelB signal peptide + His10 + maltose binding pro…

   Expression

   Bacterial

   Mutation

   mature HtrA: lacks the HtrA signal peptide

   Promoter

   T7lac

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST26 Ago2

   Plasmid

   #59904

   Purpose

   Expresses 6xHis-Ago2 (N-termi tag) in mammalian cells

   Depositor

   Carl Novina

   Article

   Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

   Insert

   Argonaute 2 (AGO2 Human)
   

   Tags

   6xHis (N-termi tag)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEANHISECArdB

   Plasmid

   #96967

   Purpose

   Expression of E. coli ArdB protein wirh an N-terminal TEV protease cleavable His tag

   Depositor

   Huanting Liu, James Naismith

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   E. coli ArdB
   

   Tags

   N-ter TEV cleavable 6His

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDY0216 PASTEv1 PE2-P2A-Bxb1

   Plasmid

   #179104

   Purpose

   PASTEv1 PE2-P2A-Bxb1

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   pCMV-PE2-P2A-Bxb1
   

   Expression

   Mammalian

   Available Since

   Feb. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-myc-ERK2-L4A-MEK1_fusion

   Plasmid

   #39197

   Depositor

   Melanie Cobb

   Article

   Robinson et al Curr Biol. 1998 Oct 22;8(21):1141-50.

   Insert

   ERK2-MEK1 Fusion (MAP2K1, Mapk1 Rat, Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   L4A = L33A, L37A, L40A, L42A

   Promoter

   CMV

   Available Since

   July 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. kiCAP-AAV-MyoAAV3F

   Plasmid

   #224447

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 3F, a muscle-tropic AAV capsid in mice and macaques.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Mutation

   RGDHASW insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available Since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-EF1A-mNeonGreen2x-SREBP1-P2A-Puro

   Plasmid

   #228401

   Purpose

   Encodes a N-terminal truncated version of SREBP1 lacking part of the transcriptional activation domain and fused to a tandem of mNeongreen to visualize the import of SREBP1

   Depositor

   Dirk Daelemans

   Article

   Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

   Insert

   SREBP1 (SREBF1 Human)
   

   Tags

   mNeonGreen2x

   Expression

   Mammalian

   Mutation

   deleted N terminal activation domain (amino acids…

   Promoter

   EF1a

   Available Since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-DmrB-DmrB-Ripk3-2A-mCherry-puro

    Plasmid

    #231972

    Purpose

    Tet inducible expression of DmrB-Ripk3 with mCherry to induce Ripk3 necroptosis

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    Ripk3-deltaC dimerizing construct with bi-cistronic mCherry (Ripk3 Mouse)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. kiCAP-AAV-MyoAAV4E

    Plasmid

    #224452

    Purpose

    Rep/Cap plasmid for the production of MyoAAV 4E, a muscle-tropic AAV capsid in mice and macaques.

    Depositor

    Pardis Sabeti

    Article

    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

    Insert

    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    

    Use

    AAV

    Mutation

    RGDFNNT insert between amino acids 588 and 589 of…

    Promoter

    p41

    Available Since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUltra-Hot-PC

    Plasmid

    #184466

    Purpose

    Lentiviral vector for bi-cistronic expression of mCherry and PC (seperated by P2A)

    Depositor

    Gerardo Ferbeyre

    Article

    Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

    Insert

    Pyruvate Carboxylase (PC Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    UbC

    Available Since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-SynI-CreOn-FlpOff-Kir2.1-P2A-EGFP

    Plasmid

    #176278

    Purpose

    Viral vector for co-expression of Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

    Depositor

    Martin Riccomagno

    Article

    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    Kir2.1-P2A-EGFP (Kcnj2 Mouse, Synthetic)
    

    Use

    AAV and Cre/Lox

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    human Synapsin I

    Available Since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKK-BI16-ORF1-3C-mRuby3_ORF2-TEV-mClover3

    Plasmid

    #105802

    Purpose

    Concomitant expression of two proteins. One protein expressed with mRuby3, cleavable by 3C; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    ORF1: 3C-mRuby3; ORF2: TEV-mClover3

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-CRISPRv2-sgIl1r1_#2-hygro

    Plasmid

    #231990

    Purpose

    Knockout mouse Il1r1

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    sgRNA with Cas9 and hygromycin resistance (Il1r1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-IDO1-p2a-eGFP

    Plasmid

    #187026

    Purpose

    Expresses IDO1 and GFP (seperately) in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Maassen et al Free Radic Biol Med. 2022 Aug 1;188:287-

    Insert

    Indoleamine 2,3-dioxygenase 1 (IDO1 Human)
    

    Tags

    P2A-GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. kiCAP-AAV-MyoAAV3A

    Plasmid

    #224442

    Purpose

    Rep/Cap plasmid for the production of MyoAAV 3A, a muscle-tropic AAV capsid in mice and macaques.

    Depositor

    Pardis Sabeti

    Article

    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

    Insert

    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    

    Use

    AAV

    Mutation

    RGDYVGL insert between amino acids 588 and 589 of…

    Promoter

    p41

    Available Since

    Sept. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-SynI-CreOn-FlpOff-Kir2.1MUT-P2A-EGFP

    Plasmid

    #176279

    Purpose

    Viral vector for co-expression of non-functional Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

    Depositor

    Martin Riccomagno

    Article

    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    Kir2.1MUT-P2A-EGFP (Kcnj2 Mouse, Synthetic)
    

    Use

    AAV and Cre/Lox

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    GYG to AAA (aa144-146)

    Promoter

    human Synapsin I

    Available Since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. ATX3-13Q

    Plasmid

    #184248

    Purpose

    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)

    Depositor

    Sandra Macedo Ribeiro

    Article

    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

    Insert

    ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track (ATXN3 Synthetic, Human)
    

    Tags

    6x His-Tag and TEV cleavage site

    Expression

    Bacterial

    Mutation

    C-terminal codon optimization for protein expres…

    Promoter

    T7 promoter

    Available Since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLHCX-ATF4 mScarlet NLS

    Plasmid

    #115970

    Purpose

    ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I (retroviral vector backbone)

    Depositor

    David Andrews

    Article

    Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

    Insert

    activating transcription factor 4 (ATF4 Synthetic, Human)
    

    Use

    Retroviral

    Tags

    HA tag, c-myc NLS, and mScarlet-I

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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