We narrowed to 17,085 results for: Por

1. pMV361_MjTyrRS

   Plasmid

   #193250

   Purpose

   Expresses the M. jannaschii tyrosyl-tRNA synthetase (MjTyrRS)

   Depositor

   Jessica Seeliger, Peter Schultz

   Article

   Touchette et al ACS Infect Dis. 2017 May 12;3(5):336-348

   Insert

   MjTyrRS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   hsp60

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKT2/CAGXSP/PalmReNL

   Plasmid

   #182970

   Purpose

   Stably express PalmReNL in mammalian cells via the sleeping beauty transposon system.

   Depositor

   Masamitsu Kanada

   Article

   Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

   Insert

   PalmReNL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available Since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-sACt-mCherry-NLS

   Plasmid

   #138214

   Purpose

   Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; nuclear targeted.

   Depositor

   Jin Zhang

   Article

   Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

   Insert

   sACt-mCherry-NLS (Adcy10 Rat)
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSF3-Flag-Cbir-Ago2_Myc-Nbir-Dicer

   Plasmid

   #90013

   Purpose

   Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-Dicer

   Depositor

   Julien Béthune

   Article

   Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

   Inserts

   CBirA*-Ago2
   NBirA*-Dicer
   

   Use

   Retroviral; Flp/frt

   Tags

   FLAG and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-Hygro-EF1A-WWE-Linker-eGFP

   Plasmid

   #176072

   Purpose

   EGFP fused to the C-terminus of a WWE domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   WWE
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223-MCU-E257A

   Plasmid

   #31727

   Depositor

   Vamsi Mootha

   Article

   Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

   Insert

   MCU (MCU Human)
   

   Use

   Pdonr gateway

   Tags

   None

   Expression

   Mutation

   Changed E257 to Alanine

   Promoter

   Available Since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-Hygro-EF1A-PBZC-Linker-eGFP

   Plasmid

   #176070

   Purpose

   EGFP fused to the C-terminus of a PBZ domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   PBZ-C
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available Since

   Feb. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-Hygro-EF1A-PBM-Linker-eGFP

   Plasmid

   #176069

   Purpose

   EGFP fused to the C-terminus of a PBM domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   PBM
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFP-Rab8a-QL

   Plasmid

   #86076

   Purpose

   A mammalian expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus EGFP.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   hRab8A (RAB8A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Changed glutamine 67 to leucine

   Promoter

   CMV

   Available Since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSC-cUnaG-P2A-nUnaG-ST-P2A-mCh

    Plasmid

    #207634

    Purpose

    A plasmid encoding photocaged SpyCatcher (pSC)-cUnaG, nUnaG-SpyTag (ST), and mCherry separated by P2A cleaving sequences for mammalian expression.

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    photocaged SpyCatcher-cUnaG-P2A-nUnaG-SpyTag-P2A-mCh
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Amber stop codon at SC's critical lysine

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSF3-Flag-Cbir-Ago2_Myc-Nbir-GFP

    Plasmid

    #90015

    Purpose

    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-GFP

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    CBirA*-Ago2
    NBirA*-GFP
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pB.GFP-KIF1A tail 395-1698

    Plasmid

    #134618

    Purpose

    Mammalian expression of mouse KIF1A 395-1698

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF1A (Kif1a Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 394 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221-eGFP_mito

    Plasmid

    #221414

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    eGFP_mito
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. PGK ARF6(Q67L)-CFP

    Plasmid

    #200428

    Purpose

    Mammalian expression of Q67L mutation in ARF6

    Depositor

    Erika Holzbaur

    Article

    Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

    Insert

    ARF6 (ARF6 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Q67L

    Promoter

    PGK

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. TRE-UBC-hCD46-T2A-GFP

    Plasmid

    #205457

    Purpose

    lentiviral plasmid for expression of human CD46

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    hCD46 (CD46 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. EGFP-Rab8a-TN

    Plasmid

    #86077

    Purpose

    A mammalian expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    hRab8A (RAB8A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed threonine 22 to aspargine

    Promoter

    CMV

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiCRISPRv1_SDHB

    Plasmid

    #177981

    Purpose

    lentiviral vector expressing Cas9 and a sgRNA targeting SDHB

    Depositor

    David Sabatini, Jessica Spinelli

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    sgRNA targeting SDHB (SDHB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    U6

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. TetO-FUW-PLZF

    Plasmid

    #61543

    Purpose

    Expresses PLZF in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    PLZF (ZBTB16 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    I380T and G580D

    Promoter

    Available Since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-RYK-V5/HIS

    Plasmid

    #205610

    Purpose

    Expression of human RYK receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    RYK receptor tyrosine kinase (RYK Human)
    

    Use

    Tags

    V5-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNLF1-C_hCOL4A5-Nluc_R1683X

    Plasmid

    #198124

    Purpose

    Express hCOL4A5_R1683X in mammalian cells

    Depositor

    Jeffrey Miner

    Article

    Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

    Insert

    COL4A5 (COL4A5 Human)
    

    Use

    Tags

    Nluc

    Expression

    Mammalian

    Mutation

    R1683X

    Promoter

    CMV, beta-globin/IgG chimeric intron

    Available Since

    April 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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