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pAAV-Syn-VARNAM2-Kv2.1PR

Plasmid

#195524

Purpose

Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted

Depositor

Vincent Pieribone

Publication

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

VARNAM2-Kv2.1 proximal restriction sequence

Use

AAV

Expression

Mammalian

Mutation

VARNAM SI linker

Promoter

Synapsin

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPRai

Plasmid

#189938

Purpose

Expresses CRISPRai cassette under EF1a promoter

Depositor

Yu-Chen Hu

Publication

Truong et al Nucleic Acids Res. 2019 Jul 26;47(13):e7

Insert

CRISPRai cassette

Expression

Mammalian

Promoter

CMV enhancer-rat EF1 alpha

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LT2_7_OxPro

Plasmid

#177709

Purpose

Plasmid for genome integration in X2 site expressing OxPro (oxidative stress biosensor)

Depositor

Lisbeth Olsson

Publication

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Inserts

pTRX2_5xUAS-ymYPET-tCYC1
pTEFmut8-mCherry-tADH1

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_CDK4(R24C)-P2A-Hygro_Barcode

Plasmid

#170222

Purpose

Barcoded lentiviral vector to express CDK4 (R24C) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Publication

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CDK4 (CDK4 Human)

Use

Lentiviral

Mutation

R24C

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 B.1.351v1

Plasmid

#169462

Purpose

Encodes SARS-CoV-2 B.1.351v1 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Publication

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.351v1 Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCMV-LOx-IRES-EGFP

Plasmid

#166877

Purpose

Express the bacterial lactate oxidase (LOx) enzyme in mammalian cells

Depositor

Sergey Kasparov

Publication

Vaccari Cardoso et al Glia. 2021 Jan 5. doi: 10.1002/glia.2396

Insert

Lactate oxidase

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pDGE463

Plasmid

#153234

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Publication

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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Omega Destination-CMV::ELuc:bGH

Plasmid

#124528

Purpose

Omega destination vector, that has a CMV:ELUC:bgH in the backbone

Depositor

Koen Venken

Publication

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Type

Empty backbone

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/CUG-nLuc-3XFLAG

Plasmid

#127300

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/GUG-nLuc-3XFLAG

Plasmid

#127301

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pEG302 22aa SunTag VP64 gRNA4 (FWA)

Plasmid

#120249

Purpose

CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA

Depositor

Steven Jacobsen

Publication

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJB02

Plasmid

#111959

Purpose

Expresses GST-GFP-DHFR; construct generated for pull-down and localization experiments

Depositor

Matthew Good

Publication

Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

Inserts

mEGFP
eDHFR

Tags

DHFR

Expression

Bacterial

Mutation

eDHFR;residues 2-159 and enhanced GFP with monome…

Promoter

T7

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pmiRFP-beta-Actin-C-19

Plasmid

#108412

Purpose

Beta-actin fused to monomeric near-infrared fluorescent protein miRFP

Depositor

Edward Boyden

Publication

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

beta-Actin

Tags

miRFP

Expression

Mammalian

Promoter

CMV

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pZac2.1 CMV173-SaCas9-U6-SaDMDR7-U6-SaDMDL2

Plasmid

#78606

Purpose

pZac2.1 CMV173-SaCas9-U6-SaDMDR7-U6-SaDMDL2

Depositor

Amy Wagers

Publication

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

CMV173-SaCas9-U6-SaDMDR7-U6-SaDMDL2

Use

AAV and CRISPR

Tags

HA tag

Expression

Mammalian

Promoter

173CMV, U6

Available since

Dec. 16, 2017

Availability

Academic Institutions and Nonprofits only
pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)

Plasmid

#101105

Purpose

Encodes dCas9(C) (without VP64) fused to VEGFR1

Depositor

Tudor Fulga

Publication

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

VEGFR1-dCas9(C)

Use

CRISPR and Synthetic Biology

Tags

HA

Expression

Mammalian

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pRS423-FjTAL(JBEI-15466)

Plasmid

#87936

Purpose

Expression in S.cerevisiae of FjTAL

Depositor

Dominique Loque

Publication

Eudes et al Microb Cell Fact. 2016 Nov 21;15(1):198.

Insert

FjTAL

Expression

Yeast

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pME-EGFP-P2A-iCre (JDW 921)

Plasmid

#224519

Purpose

A Gateway compatible middle entry clone containing EGFP P2A iCre (codon optimized cre)

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EGFP-P2A-iCre

Use

Cre/Lox

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pME-Sun1-2xsfGFP-6xMYC-pA (JDW 681)

Plasmid

#224489

Purpose

Gateway compatible middle entry clone containing Sun1 reporter fusion to 2 copies of sfGFP and 6 MYC tags (Nuclear envelope reporter and affinity tag)

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Sun1-2xsfGFP-6xMYC-pA

Use

Gateway Cloning

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pME Lamin B Vhh mNeonGreen-pA (JDW 1312)

Plasmid

#224501

Purpose

Gateway compatible middle entry clone containing Lamin B nanobody fused to mNeonGreen with HA Tag (For visualizing the nuclear lamina, lamin chromobody)

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Lamin B Vhh mNeonGreen-pA

Use

Gateway Cloning

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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LPAR2-DuET

Plasmid

#213330

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Publication

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

LPAR2 (LPAR2 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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