We narrowed to 14,474 results for: shi

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pMK446 (BSD-P2A-BromoTag-mClover)

Plasmid

#214376

Purpose

BromoTag-mClover for N-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

BSD-P2A-BromoTag-mClover

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMK447 (BSD-P2A-BromoTag-mCherry)

Plasmid

#214377

Purpose

BromoTag-mCherry for N-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

BSD-P2A-BromoTag-mCherry

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V1)

Plasmid

#55222

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

SNAPf and TwinStrepII

Expression

Insect

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pAB061i3

Plasmid

#79230

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Ppro1 rpoZ; PlacIq lacI

Expression

Bacterial

Promoter

Ppro1; PlacIq

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR223-BOLF1

Plasmid

#198579

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BOLF1

Use

Gateway entry vector

Mutation

None (wildtype)

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pSUPERretro-Sirt7 shRNA2

Plasmid

#53145

Purpose

shRNAi vector for Sirt7

Depositor

Katrin Chua

Article

Barber et al Nature. 2012 Jul 5;487(7405):114-8. doi:

Insert

Sirt7

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

H1 RNA polymerase III

Available Since

May 9, 2014

Availability

Academic Institutions and Nonprofits only
pSUPERretro-Sirt7 shRNA1

Plasmid

#53144

Purpose

shRNAi vector for Sirt7

Depositor

Katrin Chua

Article

Barber et al Nature. 2012 Jul 5;487(7405):114-8. doi:

Insert

Sirt7

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

H1 RNA polymerase III

Available Since

May 9, 2014

Availability

Academic Institutions and Nonprofits only
pMAH-EcaYRS

Plasmid

#153558

Purpose

Amber suppression for incorporating 3-aminotyrosine to proteins in mammalian cells

Depositor

Huiwang Ai

Article

Zhang et al Nat Chem Biol. 2020 Sep 14. pii: 10.1038

Inserts

EcaYRS
amber suppression tRNA (multiple copies)

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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TREER-Actin

Plasmid

#208330

Purpose

TREER genetically-encoded tag introduced before actin; to be used with TinyERRE probe peptide.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TREER-Actin

Tags

TREER

Expression

Mammalian

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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TOMM20-PuRRRE

Plasmid

#208329

Purpose

PuRRRE genetically-encoded tag introduced after TOMM20; to be used with PunyEEER probe peptide.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TOMM20-PuRRRE

Tags

PuRRRE

Expression

Mammalian

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAB064d

Plasmid

#79197

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Plac62 luxAB; Ppro1 434cI-SH2ABL1

Expression

Bacterial

Promoter

Plac62; Ppro1

Available Since

Dec. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pPB-R1R2_EF1ap300dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#113343

Purpose

Expresses p300-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

Depositor

Gavin Wright

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

p300core-dCas9-VP64-T2A-MS2-p65-HSF1

Use

Piggybac

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV Puro-pGK:GFP

Plasmid

#68486

Purpose

Retrovirus expressing GFP driven by pGK promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Puromycin Resistance
GFP

Use

Retroviral

Expression

Mammalian

Promoter

LTR and pGK

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pMK487 (Hygro-P2A-3mAID)

Plasmid

#214399

Purpose

3mAID for C-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

Hygro-P2A-3mAID

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMK450 (BromoTag-mCherry-BSD)

Plasmid

#214380

Purpose

BromoTag-mCherry for N-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

BromoTag-mCherry-BSD

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAB082a

Plasmid

#79233

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Ppro1 rpoZ-Cry1Ac-d123

Expression

Bacterial

Promoter

Ppro1

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pTK-Snail

Plasmid

#36976

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

Snail (Snai1 Mouse)

Use

Lentiviral

Available Since

May 31, 2012

Availability

Academic Institutions and Nonprofits only
pVB3011

Plasmid

#113389

Purpose

Galactose-inducible L-BC gag: cure killer

Depositor

Suzanne Sandmeyer

Article

Bilanchone et al PLoS Genet. 2015 Sep 30;11(9):e1005528.

Insert

L-BC Gag

Expression

Yeast

Mutation

Please see depositor comments

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pT-GEM(2)

Plasmid

#62894

Purpose

Trojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Type

Empty backbone

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR223-GFP

Plasmid

#198589

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

GFP

Use

Gateway entry vector

Mutation

None (wildtype)

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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