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pCfB3048(gRNA XII-2)

Plasmid

#73290

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-2

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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enAsABE8e-dimer

Plasmid

#138507

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(WT)-ecTadA(8e)-dAsCas12a (En)

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX Eat2-SH2

Plasmid

#46423

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Eat2 (SH2D1B Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1-132

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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TAL2288

Plasmid

#36723

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FANCG-TALEN_Left (Fancg Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pGWB427

Plasmid

#74821

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with T7 (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Sept. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB426

Plasmid

#74820

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with T7 (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pZ_lacO-cP-AOX1-eGFP

Plasmid

#126720

Purpose

yeast plasmid overexpressing eGFP

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

egfp

Tags

6xHis,Myc

Expression

Yeast

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-ChRger1-TS-EYFP

Plasmid

#127244

Purpose

High photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

ChRger1-TS-EYFP

Use

AAV

Tags

EYFP and SpyTag

Expression

Mammalian

Promoter

CamKIIa

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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TAL2306

Plasmid

#36734

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

ERCC2-TALEN_Left (Ercc2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

July 18, 2012

Availability

Academic Institutions and Nonprofits only
TAL2254

Plasmid

#36697

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FANCC-TALEN_Left (Fancc Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 FYN

Plasmid

#67106

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

FYN (FYN Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pTH33 [pmyo-3::YFP-BeCyclOp(A-2x)::SL2::mCherry]

Plasmid

#168169

Purpose

Expression of YFP-BeCyclOp(A-2x) in BWMs of C. elegans

Depositor

Alexander Gottschalk

Article

Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

Insert

YFP-BeCyclOp(A-2x) (codon optimized for C. elegans)

Tags

YFP

Expression

Worm

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX PLCg1(N)-SH2

Plasmid

#46470

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

PLCg1(N)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 539-664

Promoter

Tac

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pLV-shMdk-334

Plasmid

#99025

Purpose

Expresses shRNA targeting mouse Mdk (midkine)

Depositor

Amy Lasek

Article

Chen et al Genes Brain Behav. 2017 Apr 11. doi: 10.

Insert

Mdk shRNA (Mdk Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

mouse U6

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pGWB422

Plasmid

#74816

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with GST (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 CREBZF

Plasmid

#67070

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

CREBZF (CREBZF Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pGWB428

Plasmid

#74822

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with TAP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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TAL2322

Plasmid

#36748

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FOXO1-TALEN_Left (FOXO1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
TAL2268

Plasmid

#36705

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FOXO3-TALEN_Left (Foxo3 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2269

Plasmid

#36706

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FOXO3-TALEN_Right (Foxo3 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only